EFFECTIVITY AND PHYSICOCHEMICAL STABILITY OF NANOSTRUCTURED LIPID CARRIER COENZYME Q10 IN DIFFERENT RATIO of LIPID ALFA CETYL PALMITATE AND ALPHA TOCOPHERYL ACETATE AS CARRIER

Abstract

Objective: The aim of this study was to investigate physical characteristics of nanostructured lipid carriers (NLCs) mixture of alpha tocopheryl acetate, cetyl palmitate, Tween 80 and propylene glycol using high shear homogenization technique on NLC preparation to predict the optimum ratio of alpha tocopheryl acetate-cetyl palmitate to produce good characteristics of NLC loaded coenzyme, higher % EE, good penetration, controlled release, and stable.Methods: Lipid characterizations were conducted by diffraction scanning calorimetry, X-ray diffraction, and Fourier transforms infrared spectrophotometry. Coenzyme Q10 concentration was measured by spectrophotometer at 275 nm. NLC characteristics based on their morphology was determined using transmission electron microscope, particle size, and its polydispersity index which were measured with Delsa Nano™ particle size analyzer. Percentage of coenzyme Q10 entrapped in NLC was determined by dialysis bag method. Coenzyme Q10 release profile was measured using with Franz cell for 12 hrs. The penetration depth of NLC coenzyme Q10 in abdominal skin of Wistar rat was determined with fluorescence microscopy using rhodamine B as marker. NLC physical stability based on minimum of particle size variation, pH and viscosity during 90 days storage. Results:The result showed that formula with ratio of cetyl palmitate-alpha tocopheryl acetate 70:30 (% w/w) produce good characteristics of NLC loaded coenzyme, higher % EE, good penetration, controlled release, and stable in 90 days storage. Conclusion:The coenzyme Q10 NLC system with cetyl palmitate and alpha tocopherol acetate as lipid matrixare characterized by small particle size, low crystallinity, spherical morphology of particle and high coenzyme Q10 entrapment efficiency. Crystal modification led to the formation of a more amorphous thereby increasing the drug entrapment

An Overview on Topical Administration of Carotenoids and Coenzyme Q10 Loaded in Lipid Nanoparticles

“…Coenzyme Q10 (CoQ10), also known as ubiquinone or ubidecarenone, is a liposoluble vitamin-like compound that plays a relevant role in several biochemical processes, such as the production of cellular energy in the form of adenosine triphosphate, the mitochondrial electron transport chain, and the inhibition of cell membrane peroxidation [23,[124][125][126][127]. CoQ10 is part of a homologous series of substances that share a common benzoquinone ring structure, differing in the length of the isoprenoid side chain.…”

“…CoQ10 is part of a homologous series of substances that share a common benzoquinone ring structure, differing in the length of the isoprenoid side chain. The Q in CoQ10 name refers to the quinone group and, the number, the isoprene units in the side chain; each isoprene unit contains 5 carbons [126][127][128][129][130]. In humans and a few other mammalian species, the side chain contains 10 isoprene units [129,131].…”

Influence of Formulation Factors on the Size of Nanostructured Lipid Carriers and Nanoemulsions Prepared by High Shear Homogenization

“…NLCs made out of a strong lipid matrix with a certain content of a fluid lipid are another era of lipid nanoparticles. NLCs are viewed as a more advanced era of nanoparticles, which have enhanced properties for drug loading, adjustment of the delivery profile, and stable medication during storage [8][9][10][11]. Because of the lipophilic content of the NLCs, they are considered especially helpful in the administration of lipophilic medications.…”

Effect of Combination Soy Bean Oil and Oleic Acid to Characteristic, Penetration, Physical Stability of Nanostructure Lipid Carrier Resveratrol

“…This was because the NLC RSV F1 had a small particle size. Small particle size caused the structure between the particles became tight so that the occlusivity increased, in which also increased occlusivity that affected the penetration of active ingredients into the skin (Putranti et al 2017). In addition, the solubility of active ingredients in lipids also affected penetration, evidenced with how soy bean oil liquid lipids were more soluble in resveratrol than in F1 formula.…”