Effectiveness of Withania frutescens root extract on testicular damage induced by lead acetate in adult albino rats

Bentaiba, Karima; Belhocine, Mansouria; Fadela, Chougrani; Bououdina, M.; Mostari, Abbassia; Fernini, Meriem; Mohammed, Bouzouina

doi:10.1016/j.reprotox.2022.12.006

Cited by 7 publications

(8 citation statements)

References 37 publications

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“…In the current study, LA-cessation rats (Group II(a)) showed reductions in SOD, CAT, GPx, and GSH activities and elevations in MDA concentration in tissue homogenates when compared with the untreated control rats. The present results agree with previous observations explaining that long-term exposure to lead causes oxidative damage by increasing lipid peroxidation (elevated MDA levels), inhibiting SOD, CAT, and GPX activity, and lowering GSH levels in the testes [10,34,5,35], reduced activities of antioxidant enzymes are frequently implicated in oxidative stress. Rats exposed to LA were reported to exhibit significantly lower levels of testicular antioxidant enzymes.…”

Section: Discussionsupporting

confidence: 93%

“…These plant-based remedies have fewer adverse effects, are affordable, and are easily accessible. Current research has focused on the protective impact of antioxidant-rich plant compounds and medicinal herbs, such as curcumin, in preventing free radical-induced tissue damage [10]. The use of plant-derived polyphenol flavanone, which has no adverse effects and is abundant in nature, has piqued interest in several dietary approaches.…”

Section: Introductionmentioning

confidence: 99%

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Improvement of Lead Acetate-Induced Testicular Oxidative Damage by Vitis Vinifera (Linn.) Seed Extract In Adult Wistar Rats

2023

JAZ

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Introduction: Lead is the most ubiquitous hazardous toxin in the environment. It’s a severe threat to public health and especially to the male reproductive system. In recent years, the usage of the antioxidant to reduce the toxicity of heavy metals has expanded globally. Antioxidants can prevent or minimise the oxidation of other molecules by ROS in a tissue or cell. Grape seeds are natural, rich sources of antioxidant compounds. Aim/Objective: The present study was undertaken to investigate the effect of GSE (grape seed extract) against lead acetate-induced testicular oxidative damage on testis of Wistar rats. Materials and Methods: 24 male Wistar rats were used in the study. They were split into two groups: Group I was the control group (6 rats), and Group II had 18 rats that were given LA at 50 mg/kg BW for 28 days. On the 28th day, all 18 rats were subdivided into three groups: Group II(a) (LA Cessation), Group II(b), and Group II(c) treated with GSE once a day, orally, up to the 56th day. A preliminary phytochemical analysis was conducted. The testicular weight, enzymatic and non- enzymatic oxidative stress markers were estimated. Results: The phytochemical screening showed that bioactive compounds present in the GSE, including phenols, tannins, flavonoids, anthocyanin, glycosides, triterpenoids, and alkaloids. In addition, there was a significant decrease in SOD, CAT, GPx, and GSH levels and a significantly elevated MDA concentration. However, post-treatment with GSE significantly restored the testicular oxidative damage caused by LA in the testis. Conclusion: We concluded that the GSE may have the potential to provide a promising therapeutic effect against LA-induced testicular toxicity.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Improvement of Lead Acetate-Induced Testicular Oxidative Damage by Vitis Vinifera (Linn.) Seed Extract In Adult Wistar Rats

2023

JAZ

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“…On administration of W. frutescens, the rats exhibited no toxic effects; furthermore, the testosterone level and sperm count also significantly increased. 30 The treatment groups showed mild deteriorations in seminiferous tubules and significant improvement in active spermatogenesis. Furthermore, the therapeutic effect of WS has resulted in the absence of atrophy, vacuolation, and necrosis of the testicular cells.…”

Section: Discussionmentioning

confidence: 91%

Therapeutic Potential of Withania somnifera on Nandrolone Decanoate Induced Gonadal Toxicity

Sivanandam,

Senthilkumar,

Dipti

et al. 2024

Ind. J. Pharm. Edu. Res

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Background: Nandrolone decanoate (ND) is a commonly used androgen-based steroid that causes adverse effects on illicit self-administered users to boost their physical endurance. Although, it has an anabolic effect on the skeletal muscles, plausible evidence supports that the gonadal toxicity of ND can cause damage to reproductive functions. Aim: The present study investigated the possible protective effects of Withania somnifera (WS) root extract (200 and 400 mg/kg bw) on ND-induced (10 mg/kg bw) male gonadal toxicity in rats. Materials and Methods:The present seminal research was not experimented in any of the earlier studies. ND was induced in rats by intramuscular injection bi-weekly for 30 days. The aqueous extract of WS root powder was delivered orally once daily for 30 days to the ND-treated group. Hematoxylin-and-eosin-marked sections of the testes were studied. Results: The testosterone levels significantly decreased while there was an increase in the malondialdehyde levels among the ND-induced group against the control group. The ND induced rats treated with varying doses of WS showed a significant reversal of the gonadal toxicity by improvement in the testosterone and antioxidant parameters. The sperm count and sperm motility rate were increased significantly after WS therapy. Furthermore, the testis morphology of the WS-treated group indicated preserved germinal cells, active spermatogenesis, and absence of atrophy, vacuolation, and necrosis. Conclusion: WS treatment in ND-induced male rats alleviated the toxic effects of the ND on the gonads, probably due to its anti-inflammatory and antioxidant properties.

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“…The reducing power of EO was determined according to Bentaiba et al 33 In this experiment, 250 µL of EO ethanolic solutions at different concentrations were mixed with 625 µL of phosphate buffer (0.2M, pH 6.6) and 625 µL of 1% potassium ferrocyanide K3Fe (CN) 6. The mixture was incubated at 50°C in a water bath for 20 min, cooled to room temperature, and then 625 µL of trichloroacetic acid (10%) was added to stop the reaction.…”

Section: Frap Assaymentioning

confidence: 99%

Chemical Composition and Pharmacological Activities of Calamintha nepeta Essential Oil

2024

TJNPR

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Calamintha nepeta (Lamiaceae), widely distributed throughout the Mediterranean area, is a small and fragrant perennial herb traditionally used for both culinary and medicinal purposes. It serves as an antiseptic, spasmolytic, diuretic, and cough suppressant. The objective of this research is to characterise the chemical constituents of Calamintha nepeta essential oil (EO) through GC-MS analysis and evaluate its antioxidant and antimicrobial properties against nine multidrug resistant bacterial strains and one yeast. The study also conducted an in vitro anti-inflammatory 5lipoxygenase inhibition test and determined its lethal dose 50 (LD50) as well as the effects against carrageenan-induced paw edema in vivo. Analysis revealed that the primary components of C. nepeta included oxygenated monoterpenes, notably pulegone (58.36%), isoborneol (10.40%), menthone (8.91%), and piperitenone (3.86%). Antimicrobial tests demonstrated significant inhibition zones ranging from 10.2 to 37.12 mm. Essential oil exhibited minimum inhibitory concentrations and bactericidal/fungicidal concentrations ranging from 0.937 to 3.75 µL/mL and 0.937 to 15 µL/mL, respectively. Furthermore, it showed potent antioxidant activity against DPPH and a high total reducing power. Acute oral toxicity tests in Wistar rats indicated an LD50 of 2500 mg/kg. C. nepeta EO also exhibited strong anti-inflammatory properties against carrageenaninduced paw edema, with an IC50 value of 17.23 ± 0.32 μg/mL compared to diclofinac. Interestingly, this research highlights the importance of the phytochemical and antimicrobial properties of the essential oil of the Calamintha nepeta and accentuats its importance in pharmacological applications for treating inflammatory conditions.

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Effectiveness of Withania frutescens root extract on testicular damage induced by lead acetate in adult albino rats

Cited by 7 publications

References 37 publications

Improvement of Lead Acetate-Induced Testicular Oxidative Damage by Vitis Vinifera (Linn.) Seed Extract In Adult Wistar Rats

Improvement of Lead Acetate-Induced Testicular Oxidative Damage by Vitis Vinifera (Linn.) Seed Extract In Adult Wistar Rats

Therapeutic Potential of Withania somnifera on Nandrolone Decanoate Induced Gonadal Toxicity

Chemical Composition and Pharmacological Activities of Calamintha nepeta Essential Oil

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