Effectiveness of ultrasound for the destruction of Mycobacterium sp. strain (6PY1)

Bsoul, Abeer Al; Magnin, Jean‐Pierre; Commenges-Bernole, Nadine; Gondrexon, Nicolas; Willison, John C.; Pétrier, Christian

doi:10.1016/j.ultsonch.2009.04.005

Cited by 40 publications

(15 citation statements)

References 16 publications

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“…Our results indicated that the killing rate was independent of the initial bacterial number. Al Bsoul et al investigated the effect of initial cell concentrations on the destruction process and also found no significant effect on the disinfection rate (36). It was possible that with a higher bacterial density, the mechanical and chemical energy generated by ultrasonic waves could act on a higher proportion of bacteria.…”

Section: Enumeration Of Surviving Cells By Plate Counts (Cultivability)mentioning

confidence: 99%

Evaluation of Ultrasound-Induced Damage to Escherichia coli and Staphylococcus aureus by Flow Cytometry and Transmission Electron Microscopy

Ahn

Liu

et al. 2016

Appl Environ Microbiol

146

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bAs a nonthermal sterilization technique, ultrasound has attracted great interest in the field of food preservation. In this study, flow cytometry and transmission electron microscopy were employed to investigate ultrasound-induced damage to Escherichia coli and Staphylococcus aureus. For flow cytometry studies, single staining with propidium iodide (PI) or carboxyfluorescein diacetate (cFDA) revealed that ultrasound treatment caused cell death by compromising membrane integrity, inactivating intracellular esterases, and inhibiting metabolic performance. The results showed that ultrasound damage was independent of initial bacterial concentrations, while the mechanism of cellular damage differed according to the bacterial species. For the Gram-negative bacterium E. coli, ultrasound worked first on the outer membrane rather than the cytoplasmic membrane. Based on the double-staining results, we inferred that ultrasound treatment might be an all-or-nothing process: cells ruptured and disintegrated by ultrasound cannot be revived, which can be considered an advantage of ultrasound over other nonthermal techniques. Transmission electron microscopy studies revealed that the mechanism of ultrasound-induced damage was multitarget inactivation, involving the cell wall, cytoplasmic membrane, and inner structure. Understanding of the irreversible antibacterial action of ultrasound has great significance for its further utilization in the food industry. With increasing demands for safe, nutritious, and minimally processed products, ultrasound has attracted great interest as an alternative nonthermal technology for microbial inactivation in food preservation (1, 2). Since the 1960s, many studies have been conducted to investigate the bactericidal effects and mechanisms of ultrasound (3-5). At present, acoustic cavitation is most widely accepted as the mechanism of sterilization by highpower ultrasound (frequencies between 18 kHz and 100 kHz). The formation, growth, and collapse of cavitation bubbles in liquid media cause mechanical effects (microstreaming, high shear force, shock waves) and sonochemical reactions (free radicals, hydrogen peroxide), eventually resulting in the impairment or disruption of bacterial cells (6-8). The inhibitory effects of ultrasound on microbial cells are multifactorial, including pore formation, cell wall thinning, cell membrane disruption, release of cytoplasm contents, and damage to DNA structure (9, 10). However, there is still no consensus about the primary effect of ultrasound that causes cell death. Most researchers have argued that the primary target of ultrasound is the cytoplasmic membrane, which consists of lipoprotein layers (11, 12). Ananta et al., on the other hand, suggested that ultrasound-induced cell death might not be related to cytoplasmic membrane damage (13).There have been numerous studies on the antimicrobial efficacy of ultrasound in the food industry, which have been reviewed by Awad et al. Unfortunately, in order to determine the impact of ultrasound treatment, most of...

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Section: Enumeration Of Surviving Cells By Plate Counts (Cultivability)mentioning

confidence: 99%

Evaluation of Ultrasound-Induced Damage to Escherichia coli and Staphylococcus aureus by Flow Cytometry and Transmission Electron Microscopy

Ahn

Liu

et al. 2016

Appl Environ Microbiol

146

View full text Add to dashboard Cite

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“…6c. Increasing US power results in higher particle breakage [64] and more efficient removal, in the high frequencies [59,60]. In our system, decreasing the power from 20 to 10 W, and consequently, the power-to-volume ratio, decreased the efficiency, although in a non-linear, cost-effective manner; 50% reduction did not result to 50% decrease of the inactivation, but to 33%, although the main target is total inactivation.…”

Section: Fenton and Sonication Factorsmentioning

confidence: 65%

“…Finally, although literature suggests that modifying the sonication volume has not a significant effect on the efficiency of the sonication process (if the power-to volume ratio is respected) for bacterial inactivation [59,60], in Fig. 4c we observe 2 and 3 log 10 U of difference, respectively.…”

Section: Hydraulic Parametersmentioning

confidence: 76%

Ultrasound enhancement of near-neutral photo-Fenton for effective E. coli inactivation in wastewater

Giannakis

Papoutsakis

Darakas

et al. 2015

Ultrasonics Sonochemistry

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a b s t r a c tIn this study, we attempt for the first time to couple sonication and photo-Fenton for bacterial inactivation of secondary treated effluent. Synthetic wastewater was subjected to sequential high-frequency/low power sonication, followed by mild photo-Fenton treatment, under a solar simulator. It was followed by the assessment of the contribution of each component of the process (Fenton, US, hv) towards the removal rate and the long-term survival; sunlight greatly improved the treatment efficiency, with the coupled process being the only one to yield total inactivation within the 4-h period of treatment. The short-term beneficial disinfecting action of US and its detrimental effect on bacterial survival in long term, as well as the impact of light addition were also revealed. Finally, an investigation on the operational parameters of the process was performed, to investigate possible improvement and/or limitations of the coupled treatment; 3 levels of each parameter involved (hydraulic, environmental, US and Fenton) were tested. Only H 2 O 2 increased improved the process significantly, but the action mode of the joint process indicated potential cost-effective solutions towards the implementation of this method.

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“…Air bubbles are generated and they liberate a variable amount of energy when collapse, causing damage to the cell structure. Furthermore, highly reactive radicals, such as ·OH and ·H, are formed inside the bubbles are released to the medium, attacking the microorganism structure and causing cell death [25].…”

Section: Alternatives To Prevent Microbial Growthmentioning

confidence: 99%

Assessment of biocides and ultrasound treatment to avoid bacterial growth in diesel fuel

Bautista

Vargas

González

et al. 2016

Fuel Processing Technology

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Effectiveness of ultrasound for the destruction of Mycobacterium sp. strain (6PY1)

Cited by 40 publications

References 16 publications

Evaluation of Ultrasound-Induced Damage to Escherichia coli and Staphylococcus aureus by Flow Cytometry and Transmission Electron Microscopy

Evaluation of Ultrasound-Induced Damage to Escherichia coli and Staphylococcus aureus by Flow Cytometry and Transmission Electron Microscopy

Ultrasound enhancement of near-neutral photo-Fenton for effective E. coli inactivation in wastewater

Assessment of biocides and ultrasound treatment to avoid bacterial growth in diesel fuel

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