Effectiveness of Tiazofurin (NSC 286193) in Treating Disseminated Tumor Cells and Micrometastases in Mice

Lapis, K.; Pápay, Judit; Paku, Sándor

doi:10.1159/000226848

Cited by 4 publications

(2 citation statements)

References 17 publications

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“…We have previously compared the anti-tumor effects of tiazofurin and ribavirin with doxorubicin and cytarabine, using conventional assays (37,42).…”

Section: K5 62-ns Ce Zzsmentioning

confidence: 99%

A new assay for intracellular measurement of inosine monophosphate dehydrogenase activity: a guide for better selection of patients for enzyme-targeted chemotherapy.

Marković

Marković²,

Roberts³

et al. 1994

J Histochem Cytochem.

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We developed a new cytochemical assay for identification of cells containing inosine monophosphate dehydrogenase (IMPDH) and measurement of tumor cell sensitivity to the escalating doselschedule of the IMPDH pattern-targeting drugs. The assay is based on cytochemical principles for development of DH activity markers inside morphologically classified cells, image analysis for measuring the amount of this marker, and computer assistance for data management. The assay was optimized on a human leukemia cell line (K562-NS) and reference values for enzyme activity were established. Assay specificity was determined with different substrates and enzyme inhibitors. Sensitivity depends on the measuring instrument (image analyzing system), and the precision of the biological model used for assessment of refer-

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“…We have previously compared the anti-tumor effects of tiazofurin and ribavirin with doxorubicin and cytarabine, using conventional assays (37,42).…”

Section: K5 62-ns Ce Zzsmentioning

confidence: 99%

A new assay for intracellular measurement of inosine monophosphate dehydrogenase activity: a guide for better selection of patients for enzyme-targeted chemotherapy.

Marković

Marković²,

Roberts³

et al. 1994

J Histochem Cytochem.

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“…There are many animal models used in research on tumor metastases, and in these, secondary tumors are produced either by intravascular injection of tumor cells (so-called artificial metastasis) [1][2][3][4][5][6][7][8][9][10][11][12][13][14] or by implantation of tumor cells into soft tissue [2,[15][16][17][18] or into a defined organ (so-called spontaneous metastasis) [2,19]. However, all these models reflect a certain artificiality.…”

Section: Introductionmentioning

confidence: 99%

Metastasizing lung carcinomas in Hann: Wistar rats

1992

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In Hann:Wistar rats, 25 weekly subcutaneous injections of dipentylnitrosamine (DPNA) at doses of 0, 62.5, 125 and 250 mg/kg body weight induced lung carcinomas which metastasized partly. The incidence of the primaries was 2.5, 20.0, 77.5 and 100% in the males and 0, 0, 12.5 and 57.5% in the females, respectively. The frequency of metastases was 0, 12.5, 38.7 and 72.5% in the males and 0, 0, 40.0 and 52.2% in the females, respectively. The most common metastatic sites in males and females together were: lung-associated lymph nodes (82.1%), adrenal gland (53.6%), kidney (39.3%), pancreas (32.1%), bones (16.1%), heart (12.5%), mesentery (12.5%) and CNS (8.9%). In the females, metastases were also detected in the uterus (21.4%) and ovary (42.9%). The results indicate that: (1) through subcutaneous injections of DPNA metastasizing lung carcinomas can be induced with high incidence, and (2) the metastatic pattern is very similar to human cases. Thus, this model could be useful for the study of metastasizing lung carcinomas in experimental animals.

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