Effectiveness of the Novel Herbal Medicine, KIOM-MA, and Its Bioconversion Product, KIOM-MA128, on the Treatment of Atopic Dermatitis

Abstract

This study was conducted to determine if oral administration of the novel herbal medicine, KIOM-MA, and its Lactobacillus acidophilus-fermented product, KIOM-MA128, has therapeutic properties for the treatment of atopic dermatitis (AD). Using AD-induced BALB/c mice by Ovalbumin and aluminum hydroxide, the effectiveness of KIOM-MA and KIOM-MA128 on AD was evaluated. Oral administration of KIOM-MA and KIOM-MA128 reduced major clinical signs of AD including erythema/darkening, edema/papulation, excoriations, lichenification/prurigo, and dryness. Interestingly, KIOM-MA128 more significantly improved AD-related symptoms including decrease of IgE level in the plasma as well as reduction of scratching behavior, skin severity in the AD BALB/c model. HPLC analysis showed the significant changes in the constituent patterns between KIOM-MA and KIOM-MA128. Our results suggest that both KIOM-MA and KIOM-MA128 have potential for therapeutic reagent for the treatment of AD, and further, the efficacy is significantly enhanced by L. acidophilus fermentation via increases in its indicator molecule.

Fermented Herbal Formulas KIOM-MA128 Ameliorate IL-6-Induced Intestinal Barrier Dysfunction in Colon Cancer Cell Line

“…Furthermore, our previous studies have demonstrated that KIOM-MA/MA18 have anti-inflammatory, anticancer, and antiatopic effects [13–15]. In this study, we analyzed the active ingredients of KIOM-MA/MA128 via HPLC and identified the altered eleven ingredients (Table 1).…”

“…The mobile phase was consisted of water with 0.2% acetic acid (A) and acetonitrile with 0.2% acetic acid (B). The gradient program was set as follows: 10–50% B at 0–60 min [13]. UV absorption was monitored at 203 nm and 280 nm at a flow rate of 1.0 ml/min.…”

“…Our group has indicated that KIOM-MA is a specific agent for allergic and chronic inflammatory diseases, and it is composed of several plants, such as Glycyrrhizae radix, Polygoni cuspidati radix, Sophorae radix, Cnidii rhizoma, and Arctii fructus ; these herbs have been used in traditional oriental medicine in Asia [13, 14]. We fermented MA, the product of MA128, using probiotics to improve the therapeutic efficacy.…”

“…The novel herbal medicine KIOM-MA increased the absorption and bioavailability of the active ingredients. Previous studies have demonstrated that MA128 possesses antiatopic dermatitis, antiasthma action, and anticancer [13, 15]. …”

Fermented herbal formula KIOM-MA-128 protects against acute colitis induced by dextran sodium sulfate in mice

“…Briefly, the KIOM-MA formula ( Glycyrrhizae Radix , Polygoni Cuspidati Rhizoma , Sophorae Flavescentis Radix , Cnidii Rhizoma , Arctii Fructus , Ginseng Radix Alba , Scrophulariae Radix , Zizyphi Semen , Angelica Gigantis Radix , and Saposhnikovia Radix ) was purchased from the Korea Medical Herbs Association (Yeongcheon, Korea), and the identification was confirmed by Porf. KiHwan Bae (The College of Pharmacy, Chungnam National University, Daejeon, Korea) [7, 11]. A total of 1.84 kg of this formula (the ratios of each constituent of KIOM-MA are shown in Table 1) was placed in 18.4 L of water and then extracted by boiling for 3 h at 115 °C.…”