Effectiveness of the entomopathogenic nematode Steinernema feltiae in agar gel formulations against larvae of the Colarado potato beetle, Leptinotarsa decemlineata (Say.) (Coleoptera: Chrysomelidae)

Hussein, Hany M.; Adel, Manal M.; Gelbič, Ivan

doi:10.2478/s11535-011-0090-0

Cited by 3 publications

(1 citation statement)

References 12 publications

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“…Steinernema feltiae (Filipjev, 1934) Wouts, Mráček, Gerdin & Bedding was found to be ineffective for controlling the overwintering larval populations of large European elm bark beetle Scolytus scolytus (Fabricius) (Coleoptera: Curculionidae) at the doses applied (Finney & Walker, 1979) et al, 1982). The addition of agar to the suspension increased the viability and infectivity of the nematodes, resulting in a significant reduction in the amount of leaf damage caused by L. decemlineata (Adel & Hussein, 2010;Hussein et al, 2012).…”

Section: Coleopteramentioning

confidence: 99%

A Review of the Potential Use of Entomopathogenic Nematodes to Control Above-Ground Insect Pests in South Africa

Platt

Stokwe

Malan

2020

SAJEV

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Entomopathogenic nematodes (EPNs), of the families Steinernema and Heterorhabditis, are insect parasites that have been successfully used as biological control agents of soil-based insect pests on the North American and European continents. The success of nematodes as biological control agents of the soil stages of pest insects has led to research into their use for control of above-ground insect pests. Laboratorybased studies have shown exceptionally good control, in most cases, against such pests as mealybugs, codling moth and leaf miners. As the life stages of the above-ground insect pests have not co-evolved together with those of EPNs, they are, generally, more susceptible than the soil-based life stages. However, EPNs are susceptible to desiccation and vulnerable to UV radiation, so that ensuring their survival beyond soil environments is problematic. The impetus to avoid environmental stressors can cause EPNs to seek sheltered, cryptic habitats on foliage, where their target insect pest (such as mealybugs) may be found. The current paper provides an overview of information on the application of EPNs as a biocontrol agent for the control of insect pests above ground and on foliage, with particular reference to research done in South Africa.

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Section: Coleopteramentioning

confidence: 99%

A Review of the Potential Use of Entomopathogenic Nematodes to Control Above-Ground Insect Pests in South Africa

Platt

Stokwe

Malan

2020

SAJEV

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Formulation of Steinernema yirgalemense in gel for long-term storage at room temperature

Nxitywa

Malan

2023

J Plant Dis Prot

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The use of entomopathogenic nematodes (EPNs) offers the potential of reducing the use of chemical insecticides in the agricultural industry. Hydrogels for the long-term storage of EPNs may have the potential to improve the storage ability of EPNs. In this study, infective juveniles (IJ) of a South African isolate Steinernema yirgalemense were formulated in different gels, which included a laboratory grade and consumer grade of agar, as well as STOCKOSORB® 660, and compared it with IJ in water. Their survival and pathogenicity of the IJ were investigated at 25 °C. The last instar larvae of Tenebrio molitor were used to test the pathogenicity of the IJs 6 weeks postformulation. IJs stored in 1% and 1.5% agar formulations were found to provide the best survival of 77% and 82% after 6 weeks in formulation. Results indicate consumer grade agar to be comparable with laboratory grade and can be used as a cheaper alternative for the formulation of S. yirgalemense. When comparing the STOCKOSORB® 660 formulations, 80% IJ survival was recorded in a formulation of 1 g in 50 ml of water, with 50% of the IJs surviving in a formulation of 1 g in 100 ml of water. The IJs formulated and stored in STOCKOSORB® 660 gel were found to be more virulent to Tenebrio molitor than were the IJs formulated in agar.

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Pathogenicity of Heterorhabditis bacteriophora in Ultraviolet Light–Irradiated Agar Suspension on Spodoptera frugiperda (Lepidoptera: Noctuidae) Larvae

Tesén,

Adrianzén,

Cabrera

et al. 2023

Journal of Entomological Science

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Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) is an economically important pest of variety of crops, including maize (Zea mays L.), rice (Oryza sativa L.), and cotton (Gossypium spp.). Conventional chemical insecticides are usually used for management of this pest; however, a viable alternative is the use of microbial agents or their biological products, such as entomopathogenic nematodes. Their efficacy, however, is affected by abiotic conditions including ultraviolet (UV) light and desiccation. It is therefore necessary to develop formulations that preserve or extend the viability of the agent or product while facilitating ease of application. This laboratory study was conducted to determine the pathogenicity of the nematode Heterorhabditis bacteriophora (Poinar) against S. frugiperda third-instar larvae. Infective juveniles (IJs) of the nematode were tested at a concentration of 350 IJs/ml in two substrates (water and 0.1% [w/v] agar suspension) with four periods of exposure (0, 5, 10 and 15 min) to UV radiation at 253.7 nm. Each of the eight treatments included 40 larvae, with each treatment replicated three times in a completely randomized design (two factors of type of medium and UV exposure time). We determined that the 0.1% (w/v) agar suspension, compared with the water substrate, protected the IJs from harmful UV light and thus improved the survival and pathogenicity of the IJs against third-instar S. frugiperda.

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Effectiveness of the entomopathogenic nematode Steinernema feltiae in agar gel formulations against larvae of the Colarado potato beetle, Leptinotarsa decemlineata (Say.) (Coleoptera: Chrysomelidae)

Cited by 3 publications

References 12 publications

A Review of the Potential Use of Entomopathogenic Nematodes to Control Above-Ground Insect Pests in South Africa

A Review of the Potential Use of Entomopathogenic Nematodes to Control Above-Ground Insect Pests in South Africa

Formulation of Steinernema yirgalemense in gel for long-term storage at room temperature

Pathogenicity of Heterorhabditis bacteriophora in Ultraviolet Light–Irradiated Agar Suspension on Spodoptera frugiperda (Lepidoptera: Noctuidae) Larvae

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