Effectiveness of surface enhanced Raman spectroscopy of tear fluid with soft substrate for point-of-care therapeutic drug monitoring

Yamada, Kenji; Endo, Tatsuro; Imai, Hirohiko; Kido, Michiko; Jeong, Hieyong; Ohno, Yuko

doi:10.1117/12.2214614

Cited by 4 publications

(5 citation statements)

References 6 publications

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“…The development of cheap and friendly methods for SERS implementation, as the one considered in this work, is a first step towards this aim. An interesting future perspective is constituted by the development of soft substrates for SERS implementation, properly designed by using paper or tissues embedded in metallic nanoparticles [ 13 , 32 , 33 ].…”

Section: Discussionmentioning

confidence: 99%

Characterization of Human Tear Fluid by Means of Surface-Enhanced Raman Spectroscopy

Camerlingo

Lisitskiy

Lepore

et al. 2019

Sensors

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Tears are exceptionally rich sources of information on the health status of the eyes, as well as of whole body functionality, due to the presence of a large variety of salts and organic components whose concentration can be altered by pathologies, eye diseases and/or inflammatory processes. Surface enhanced Raman spectroscopy (SERS) provides a unique method for analyzing low concentrations of organic fluids such as tears. In this work, a home-made colloid of gold nanoparticles has been used for preparing glass substrates able to efficiently induce an SERS effect in fluid samples excited by a He–Ne laser ( λ = 633 nm). The method has been preliminary tested on Rhodamine 6G aqueous solutions at different concentrations, proving the possibility to sense substance concentrations as low as few μ M, i.e., of the order of the main tear organic components. A clear SERS response has been obtained for human tear samples, allowing an interesting insight into tear composition. In particular, aspartic acid and glutamic acid have been shown to be possible markers for two important human tear components, i.e., lactoferrin and lysozyme.

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Section: Discussionmentioning

confidence: 99%

Characterization of Human Tear Fluid by Means of Surface-Enhanced Raman Spectroscopy

Camerlingo

Lisitskiy

Lepore

et al. 2019

Sensors

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“…The emergence of several successive generations of improved, cheaper and smaller computers, optical sensors and lasers allowed for fast development in the field of optical (including ultraviolet (UV) and infrared (IR)) analytical methods, such as various forms of spectroscopy, including Raman and SERS. The new analytical solutions in this area frequently offer low price, portability, modularity, high sensitivity, easy sample preparation [ 18 , 19 ], and in medical diagnostics they follow the trend of point-of-care systems [ 9 , 20 ]. Microfluidic solutions for sample handling are also becoming more frequently incorporated into such products and sometimes play a crucial role in enhancing the throughput of the analytical systems, such as, e.g., with droplet microfluidics [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

“…A large variety of SERS substrates were reported to be effective for detection of minute amounts of pharmaceuticals, toxins and pollutants, as well as other biologically important molecules [ 20 , 22 , 23 ], including various microfluidic analytical SERS devices [ 24 , 25 , 26 ]. While the suspended nanoparticles can be made chemically more reactive towards certain chemicals, or they can be made in situ [ 27 ], the signal reproducibility is notoriously hard to obtain due to the stochastic nature of the created hotspots of plasmonic oscillations [ 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

Detection of Chloroalkanes by Surface-Enhanced Raman Spectroscopy in Microfluidic Chips

Pilát

Kizovský

Ježek

et al. 2018

Sensors

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Optofluidics, a research discipline combining optics with microfluidics, currently aspires to revolutionize the analysis of biological and chemical samples, e.g., for medicine, pharmacology, or molecular biology. In order to detect low concentrations of analytes in water, we have developed an optofluidic device containing a nanostructured substrate for surface enhanced Raman spectroscopy (SERS). The geometry of the gold surface allows localized plasmon oscillations to give rise to the SERS effect, in which the Raman spectral lines are intensified by the interaction of the plasmonic field with the electrons in the molecular bonds. The SERS substrate was enclosed in a microfluidic system, which allowed transport and precise mixing of the analyzed fluids, while preventing contamination or abrasion of the highly sensitive substrate. To illustrate its practical use, we employed the device for quantitative detection of persistent environmental pollutant 1,2,3-trichloropropane in water in submillimolar concentrations. The developed sensor allows fast and simple quantification of halogenated compounds and it will contribute towards the environmental monitoring and enzymology experiments with engineered haloalkane dehalogenase enzymes.

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“…The emergence of several successive generations of improved, cheaper and smaller computers, optical sensors and lasers allowed for fast development in the field of optical (including UV and IR) analytical methods, such as various forms of spectroscopy, including Raman and SERS. The new analytical solutions in this area frequently offer low price, portability, modularity, high sensitivity, easy sample preparation, and in medical diagnostics they follow the trend of point-of-care systems [16,9]. Microfluidic solutions for sample handling are also becoming more frequently incorporated into such products and sometimes play a crucial role in enhancing the throughput of the analytical systems, such as e.g.…”

Section: Introductionmentioning

confidence: 99%

“…Large variety of SERS substrates were reported to be effective for detection of minute amounts of pharmaceuticals, toxins and pollutants, as well as other biologically important molecules [16,18,19], including various microfluidic analytical SERS devices [20][21][22]. While the suspended nanoparticles can be made chemically more reactive towards certain chemicals, or they can be made in situ [23], the signal reproducibility is notoriously hard to obtain due to the stochastic nature of the created hotspots of plasmonic oscillations.…”

Section: Introductionmentioning

confidence: 99%

Detection of Chloroalkanes by Surface-Enhanced Raman Spectroscopy in Microfluidic Chip

Pilát¹,

Kizovský²,

Ježek³

et al. 2018

Preprint

View full text Add to dashboard Cite

Optofluidics, a research discipline combining optics with microfluidics, currently aspires to revolutionize the analysis of biological and chemical samples e.g. for medicine, pharmacology, or molecular biology. In order to detect low concentrations of analytes in water, we developed an optofluidic device containing a nanostructured substrate for surface enhanced Raman spectroscopy (SERS). The geometry of the gold surface allows localized plasmon oscillations to give rise to the SERS effect, in which the Raman spectral lines are intensified by the interaction of the plasmonic field with the electrons in the molecular bonds. The SERS substrate was enclosed in a microfluidic system, which allowed transport and precise mixing of the analyzed fluids, while preventing contamination or abrasion of the highly sensitive substrate. To illustrate its practical use, we employed the device for quantitative detection of persistent environmental pollutant 1,2,3-trichloropropane in water in submillimolar concentrations. The developed sensor allows fast and simple quantification of halogenated compounds and it will contribute towards the environmental monitoring and enzymology experiments with engineered haloalkane dehalogenase enzymes.

show abstract

Effectiveness of surface enhanced Raman spectroscopy of tear fluid with soft substrate for point-of-care therapeutic drug monitoring

Cited by 4 publications

References 6 publications

Characterization of Human Tear Fluid by Means of Surface-Enhanced Raman Spectroscopy

Characterization of Human Tear Fluid by Means of Surface-Enhanced Raman Spectroscopy

Detection of Chloroalkanes by Surface-Enhanced Raman Spectroscopy in Microfluidic Chips

Detection of Chloroalkanes by Surface-Enhanced Raman Spectroscopy in Microfluidic Chip

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