Effectiveness of Sidr Honey on the prevention of ethanol-induced gatroulcerogenesis: role of antioxidant and antiapoptotic mechanism

Abstract

Background: Sider (Ziziphus spina-christi (L.) Desf.) Honey has been used for the treatment of gastrointestinal disorders including peptic ulcer. Aim of the study: The mechanism of the antiulcer effect of sider honey was studied placing emphasis on its role to block oxidative damage and apoptosis during ethanol-induced gastric ulceration in rats. The mechanism of the antiulcer effect of sider honey was studied placing emphasis on its role to block oxidative damage and apoptosis during ethanol-induced gastric ulceration in rats. Materials and methods: Experimental animals were orally treated with sidr honey (100, 250 and 500 mg/kg, respectively) or omeprazole and subsequently exposed to 95%ethanol (5 mL/Kg, orally) to induce acute gastroulcerogenesis. Effectiveness of sidr honey was evaluated using ulcer index, pH of gastric juice, mucus content, morphological analyses, glutathione assay and malondialdehyde level. The anti-apoptotic role of sidr honey was studied using immunohistochemical staining of gastric tissues using monoclonal antibodies of Bax pathway. Results: Dose-response studies in ethanol-induced ulcer indicate that sidr honey significantly blocks gastric lesions at lower dose (100 mg/kg). Lipid peroxidation and glutathione depletion were significantly inhibited by sidr honey. Sidr honey modulated the immuno-expression of mitochondrial associated protein (Bax). Conclusion: Thus, sider honey plays a considerable role in gastro protection by acting as a potent antioxidant and antiapoptotic agent. Future study is required to explore its potential clinical usage.

Comparative study of neuropharmacological, analgesic properties and phenolic profile of Ajwah, Safawy and Sukkari cultivars of date palm (Phoenix dactylifera)

“…Furthermore, acute toxicity study with date palm extract prior to our project on biological investigation of date extracts revealed extended period of sleep in test animals. All these observations prompted us, as a part of our research on Prophetic medicine (El-Ameen et al 2015; Halabi and Sheikh 2014; Maulidiani et al 2015; Taha et al 2015), to evaluate and compare neuropharmacological effects of two date cultivars growing in Madinah (Ajwah and Safawy) and one growing outside Madinah but within Saudi Arabia (Sukkari).…”

Gastroprophylactic Effects of p-Cymene in Ethanol-Induced Gastric Ulcer in Rats

“…Moreover, ethanol improved the free-radical production abilities of the stomach epithelium, and subsequently caused severe stomach epithelial damage [12,48]. Stomach mucus might perform an important role in the epithelial defense in contradiction to ethanol [1,49,50]. The results confirmed that p-Cymene defends the mucosa via concealing extra mucous contrary to extensive gastric destruction.…”

Green Synthesis of Gold Nanoparticles Using Zingiber Officinale Rhizomes Aqueous Extract and its Gastroprotective Activity Against Ethanol-Produced Stomach Ulcer in Rodents

“…[38,39] Significances of the present exercise were similar with other amendments quantity gastroprotective and antiulcer achievement gold nanoparticle and herbal composites. [4,40,41] Pre-feeding with the gold nanoparticle, challengingly impassable gastric acid likewise takeover stomach injury in rats forced feeding ethanol, characteristic gastro-protective effect gold nanoparticle easy relatively protection of gastric wall. [42,43] Designated acute inflammation is attracted through ethanol neutrophils penetration the stomach partition.…”