Effectiveness of seminal plasma in in vitro fertilisation treatment: a systematic review and meta‐analysis

Saccone, Gabriele; Sardo, Attilio Di Spiezio; Ciardulli, Andrea; Spinelli, Marialuigia; Surbek, Daniel; Wolff, Michael von

doi:10.1111/1471-0528.15004

Cited by 24 publications

(14 citation statements)

References 16 publications

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“…Another possibility could be that this protein has a direct impact on conceptus. In this sense, SP has been reported to exert a positive effect on pregnancy outcomes, specifically improving implantation and pregnancy rates in humans ( Crawford et al, 2015 ; Saccone et al, 2019 ) and even modifying embryo gene expression in pigs ( Martinez et al, 2020 ). Thus, it cannot be discarded the influence of SP-AKR1B1 on the improvement of embryo survival and even implantation, through the modulation of the uterine environment.…”

Section: Discussionmentioning

confidence: 99%

Aldose Reductase B1 in Pig Seminal Plasma: Identification, Localization in Reproductive Tissues, and Relationship With Quality and Sperm Preservation

Mateo‐Otero

Viñolas-Vergés

Llavanera

et al. 2021

Front. Cell Dev. Biol.

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Aldose reductase B1 (AKR1B1), a NADPH-dependent enzyme that belongs to the aldo-keto reductase protein superfamily, has been reported to be involved in both male and female reproductive physiology. The objectives of this study were: (1) to evaluate the concentration of SP-AKR1B1 in pig ejaculate fractions; (2) to describe the immunohistochemical localization of AKR1B1 alongside the boar genital tract; (3) to evaluate the relationship between SP-AKR1B1 and sperm quality/functionality parameters. Ejaculates from seven boars (one ejaculate per boar) were collected in separate portions [the first 10 mL of the sperm rich fraction (SRF-P1), the rest of the SRF (SRF-P2), and the post-SRF (PSRF)], and the concentration of SP-AKR1B1 was assessed using an enzyme-linked immunosorbent assay (ELISA). Immunohistochemistry and immunoblotting targeting was conducted in the reproductive tissues of these boars. Additionally, the entire ejaculates of 14 boars (one ejaculate per boar) were collected and split into three separate aliquots for: (i) SP-AKR1B1 quantification; (ii) assessment of sperm concentration and morphology; and (iii) evaluation of sperm quality and functionality parameters upon ejaculate collection (0 h) and after 72 h of liquid storage at 17°C. Concentration of AKR1B1 in the SP of SRF-P1 (458.2 ± 116.33 ng/mL) was lower (P < 0.05) than that of SRF-P2 (1105.0 ± 229.80 ng/mL) and PSRF (1342.4 ± 260.18 ng/mL). Monomeric and dimeric AKR1B1 forms were expressed alongside the reproductive tissues, except in the bulbourethral glands. No relationship between SP-AKR1B1 and sperm quality/functionality parameters was observed either at 0 h or after 72 h of storage at 17°C. In conclusion, AKR1B1 is expressed in the reproductive organs of boars (except bulbourethral glands) and a higher concentration is found in the PSRF suggesting that seminal vesicles would be the main secretory source. However, this enzyme does not appear to be related to sperm quality/functionality or to the sperm ability to withstand liquid storage at 17°C.

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Section: Discussionmentioning

confidence: 99%

Aldose Reductase B1 in Pig Seminal Plasma: Identification, Localization in Reproductive Tissues, and Relationship With Quality and Sperm Preservation

Mateo‐Otero

Viñolas-Vergés

Llavanera

et al. 2021

Front. Cell Dev. Biol.

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“…In rodents and swine, seminal plasma exposure mediates ovarian function by increasing progesterone synthesis or altering immune cells within the corpus luteum (Gangnuss et al 2004. Unprotected intercourse at the time of embryo transfer, or seminal plasma exposure during IVF treatment increases clinical pregnancy rates in women (Tremellen et al 2000, Saccone et al 2019. It is unclear if seminal plasma can facilitate the same effect on pregnancy success in cattle.…”

Section: Discussionmentioning

confidence: 99%

Effect of seminal plasma or transforming growth factor on bovine endometrial cells

et al. 2019

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Semen induces post-coital inflammation of the endometrium in several species. Post-coital inflammation is proposed to alter the endometrial environment of early pregnancy, mediate embryonic development and modulate the maternal immune response to pregnancy. In cattle, it is common for pregnancies to occur in the absence of whole semen due to the high utilization of artificial insemination. Here, we have utilized a cell culture system to characterize semen-induced expression of inflammatory mediators in bovine endometrial cells and test the efficacy of transforming growth factor beta as the active agent in mediating any such change. We hypothesize that seminal plasma-derived transforming growth factor beta increases the expression of inflammatory mediators in bovine endometrial cells. Initially, we describe a heat-labile cytotoxic effect of seminal plasma on BEND cells, and a moderate increase in IL1B and IL6 expression. In addition, we show that transforming growth factor beta is present in bovine semen and can increase the expression of endometrial IL6, whereas blocking transforming growth factor beta in semen ameliorates this effect. However, intra-uterine infusion of seminal plasma, sperm or transforming growth factor beta did not alter the endometrial expression of inflammatory mediators. We conclude that bovine semen can modulate endometrial gene expression in vitro, which is partially due to the presence of transforming growth factor beta. It is likely that additional, unidentified, bioactive molecules in semen can alter the endometrial environment. Characterizing bioactive molecules in bovine semen may lead to the development of additives to improve artificial insemination in domestic species.

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“…Some meta-analyses provided additional information about seminal plasma in clinical pregnancy rates. The meta-analysis conducted by Crawford and collaborators [ 190 ] demonstrated a significant improvement in clinical pregnancy rates, approximately 23%, while Saccone and collaborators demonstrate a 20% improvement [ 191 ]. However, other authors’ analysis concluded that seminal plasma may effectively increase clinical pregnancy rates, but the finding should be regarded with caution since there is not enough evidence to determine the impact on live births [ 192 ].…”

Section: Role Of Seminal Plasma On Fertility Techniques and Importance Of Its Antioxidants In The Female Reproductive Tractmentioning

confidence: 99%

Antioxidants Present in Reproductive Tract Fluids and Their Relevance for Fertility

et al. 2021

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Nowadays, infertility is classified as a disease of the reproductive system. Although it does not compromise the life of the individual, it can have detrimental effects on the physiological and psychological health of the couple. Male fertility evaluation is mainly focused on the analysis of sperm parameters. However, the ejaculated fluid is also composed of seminal plasma, and the study of this fluid can provide crucial information to help in the assessment of male fertility status. Total antioxidant capacity of the seminal plasma has been positively correlated with the fertility of men. Moreover, evidence highlights to a similar importance as that of female reproductive tract fluid antioxidant capabilities and female fertility. Herein, we describe the functions of seminal plasma and female reproductive tract fluids, as well as their main antioxidant components and their relationships with fertility outcomes. Additionally, this review contains the most up to date information regarding the mechanisms of the interaction between the male and the female reproductive fluids and the importance of proper antioxidant capacity for fertilization.

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Effectiveness of seminal plasma in in vitro fertilisation treatment: a systematic review and meta‐analysis

Abstract: SP at the time of oocyte pickup is associated with higher CPR.

Cited by 24 publications

References 16 publications

Aldose Reductase B1 in Pig Seminal Plasma: Identification, Localization in Reproductive Tissues, and Relationship With Quality and Sperm Preservation

Aldose Reductase B1 in Pig Seminal Plasma: Identification, Localization in Reproductive Tissues, and Relationship With Quality and Sperm Preservation

Effect of seminal plasma or transforming growth factor on bovine endometrial cells

Antioxidants Present in Reproductive Tract Fluids and Their Relevance for Fertility

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