Effectiveness of Polyclonal and Monoclonal Antibodies Prepared for an Immunoassay of the Etofenprox Insecticide

A monoclonal antibody‐based ELISA for the analysis of the insecticide flucythrinate in environmental and crop samples

“…River water sample from Rokko River, Kobe, Japan and pond water sample from Kobe University, Japan were spiked with flucythrinate dissolved in methanol, and the final methanol concentration of samples was adjusted to 100 ml litre −1 . Spiked samples were mixed with an equal volume of MAb solution prepared in a twofold concentrated assay buffer RS (20 m M Tris‐HCl buffer, 600 m M sodium chloride, pH 8), containing 10% Block Ace®, 7 g litre −1 RSA, and 5000‐fold diluted rabbit anti‐etofenprox serum (anti‐HAP1 antibody prepared by Miyake et al 11), and analyzed in ic‐ELISA.…”

“…As alternative analytical methods, immunoassays have been developed for the detection of pyrethroids such as bioallethrin,5, 6 permethrin, phenothrin,7–9 bioresmethrin,10 etofenprox,11 deltamethrin12 and fenpropathrin 13. Moreover, immunoassays for group‐specific detection have been developed by the use of antibodies raised against common structures such as the chrysanthemic acid moiety in pyrethroids 14–17…”

Determination of pesticides by enzyme immunoassay

“…By thorough screening and selection of antibody clones, which are affine to a particular determinant of the desired antigen, the undesirable activity of antibody preparations can be minimized because clones that give cross reactions can be revealed and rejected even at the early stages of screening. In some cases, monoclonal antibodies provide highly sensitive and specific analysis [115]. The hybridoma cell secretes antibodies in unlimited amounts that depend on only the stability of the cell, that is, its life time [116].…”

Agricultural Wastes

“…Two polyclonal antibodies and three monoclonal antibodies specific to the etofenprox insecticide were prepared from rabbits and mice, respectively (Miyake et al, 1998). The monoclonal antibodies were more reactive with etofenprox than the polyclonal antibodies by C-ELISA.…”