Effectiveness of Photodynamic Therapy for the Inactivation of<i>Candida</i>spp. on Dentures:<i>In Vitro</i>Study

Mima, Ewerton Garcia de Oliveira; Pavarina, Ana Cláudia; Ribeiro, Daniela Garcia; Dovigo, Lívia Nordi; Vergani, Carlos Eduardo; Bagnato, Vanderlei Salvador

doi:10.1089/pho.2011.3022

Cited by 56 publications

(58 citation statements)

References 32 publications

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“…to PDT have found conflicting outcomes. Mima et al [21] observed that when dentures were inoculated individually with different species of Candida and treated with Photogem R and LED light, C. tropicalis showed the lowest ufc/mL values after PDT, followed by C. krusei, C. albicans and C. glabrata. It has also been verified that 20 min PIT promoted higher photoinactivation of C. albicans biofilms than 5 min PIT when curcumin was used as PS [20].…”

Section: Discussionmentioning

confidence: 99%

“…Hematoporphyrin derivatives (HpD) are among the earliest type of PS used [10], and light-emitting diodes (LED) have been successfully used for PDT due to their lower cost and easier technology than lasers [25,27]. Previous studies have shown that the association of porphyrin and blue LED light resulted in reduction of C. albicans counts in disinfection of dentures [21,25,27] and was also effective in the treatment of denture-related OC [25,26]. Mima et al [23] observed that 500 mg l −1 of porphyrin and 305 J cm −2 of LED light was able to reduce 1.59 log of C. albicans in a murine model of OC [23].…”

Section: Introductionmentioning

confidence: 99%

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The influence of photodynamic therapy parameters on the inactivation ofCandidaspp:in vitroandin vivostudies

Alves¹,

Mima²,

Dovigo³

et al. 2014

Laser Phys.

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The influence of parameters of photodynamic therapy (PDT), such as pre-irradiation time (PIT), on the inactivation of Candida spp. was assessed in vitro and in vivo. Suspensions of Candida albicans, Candida tropicalis, Candida krusei and Candida glabrata were treated with Photogem R , incubated for 5, 10 or 15 min and illuminated with a blue LED light. Colonies were cultivated and log values of CFU ml −1 were analyzed by ANOVA and Kruskall-Wallis test. For in vivo evaluation, immunosuppressed mice were inoculated with C. albicans. PDT was performed on the dorsum of the tongue by topical administration of Photogem R and illumination after 5, 10 or 15 min. C. albicans was recovered from the tongue and the number of CFU ml −1 was analyzed by ANOVA and Tukey test. Animals were killed and the tongues were surgically removed for histological analysis. Susceptibility of Candida spp. suspensions to PDT was in decreasing order: C. albicans = C. tropicalis < C. krusei < C. glabrata. No significant difference was observed among the different PIT ( p > 0.05), both in vivo and in vitro. A significant reduction ( p < 0.05) of log(CFU ml −1 ) of C. albicans from tongues of mice was observed with no adverse effects in the tissue. PDT was effective to inactivate in vitro Candida spp. and for reduction of C. albicans in vivo, independently of the PIT used.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The influence of photodynamic therapy parameters on the inactivation ofCandidaspp:in vitroandin vivostudies

Alves¹,

Mima²,

Dovigo³

et al. 2014

Laser Phys.

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“…Mima et al did another in vitro study showed the effectiveness of Photogem ® induced PDT for the inactivation of different five species of Candida on maxillary complete dentures, including C. albicans, C. tropicalis, C. glabrata, C. krusei and C. dubliniensis. The results showed PDT reduced 3.99 log 10 CFU from dentures which were infected all species of Candida [94]. …”

Section: Photodynamic Therapy As An Anti-infectivementioning

confidence: 99%

Light based anti-infectives: ultraviolet C irradiation, photodynamic therapy, blue light, and beyond

Yin

Dai

Avci

et al. 2013

Current Opinion in Pharmacology

238

172

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Owing to the worldwide increase in antibiotic resistance, researchers are investigating alternative anti-infective strategies to which it is supposed microorganisms will be unable to develop resistance. Prominent among these strategies, is a group of approaches which rely on light to deliver the killing blow. As is well known, ultraviolet light, particularly UVC (200–280nm), is germicidal, but it has not been much developed as an anti-infective approach until recently, when it was realized that the possible adverse effects to host tissue were relatively minor compared to its high activity in killing pathogens. Photodynamic therapy is the combination of non-toxic photosensitizing dyes with harmless visible light that together produce abundant destructive reactive oxygen species (ROS). Certain cationic dyes or photosensitizers have good specificity for binding to microbial cells while sparing host mammalian cells and can be used for treating many localized infections, both superficial and even deep-seated by using fiber optic delivered light. Many microbial cells are highly sensitive to killing by blue light (400–470 nm) due to accumulation of naturally occurring photosensitizers such as porphyrins and flavins. Near infrared light has also been shown to have antimicrobial effects against certain species. Clinical applications of these technologies include skin, dental, wound, stomach, nasal, toenail and other infections which are amenable to effective light delivery.

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“…18 Its effective use in oral disinfection and prosthetic decontamination have been attested to by recent studies. 14,19 Additionally, its antimicrobial property by lipid membrane peroxidation could be enhanced by light at a proper wavelength. 20,21 Curcumin absorbs light in the blue wavelength range (300-500 nm) of the visible spectrum, which has a lower penetration depth into the tissue compared with red light, because of scattering and absorption by biomolecules; therefore, the use of curcumin in PACT is restricted to topical use for superficial wounds (e.g., skin and mouth).…”

Section: Introductionmentioning

confidence: 99%

Streptococcus MutansPhotoinactivation by Combination of Short Exposure of a Broad-Spectrum Visible Light and Low Concentrations of Photosensitizers

Paschoal

Pinto

Lin

et al. 2014

Photomedicine and Laser Surgery

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Objective: Investigate the photodynamic antimicrobial effect by the combination of a novel noncoherent broad spectrum visible light and low concentrations of curcumin and toluidine blue over suspensions of Streptococcus mutans. Background data: Long illumination times to activate photosensitizers (PS) and the use of high concentrations of these drugs in photodynamic antimicrobial chemotherapy (PACT) are limitations of its application as an antimicrobial technology in dental practice. Materials and methods: Planktonic suspensions of S. mutans were standardized and submitted to PACT treatment at low concentrations of curcumin (C) (0.075; 0.75 and 7.5 lM) and toluidine blue (T) (0.25; 2.5 and 25 lM) exposed to 42 J/cm 2 (12.2 sec; set power: 3.930 mW) of a white light (WL) (output wavelength range: 400-700 nm; beam diameter: 12 mm) (C + WL + and T + WL + , PACT groups; incubation time, C: 60 sec; T: 5 min); isolated effect of both C (C + WL -) and T concentrations (T + WL -); effect of light source (C -WL + and T -WL + ) and suspensions neither submitted to PS nor to light-emitting diode (LED) illumination (control groups, C -WL -and T -WL -). Aliquots of each group were diluted and cultured on blood agar plates and the number of colony-forming units (CFU)/mL was recorded, transformed into log 10 and analyzed by ANOVA and Tukey's test at a cutoff value at 0.05. Results: The groups submitted to PACT presented a bacterial reduction value of > 5-log 10 to both tested PS in comparison with control groups ( p < 0.05). PS or light source used alone demonstrated no antimicrobial effect on the number of viable bacterial counts. Conclusions: The combination of a novel noncoherent light at short illumination exposure time with low concentrations of studied PS achieved a lethal photoinactivation of S. mutans, and can be considered an effective antimicrobial in vitro approach for reducing the number of microorganisms involved with the dental caries process.

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Effectiveness of Photodynamic Therapy for the Inactivation ofCandidaspp. on Dentures:In VitroStudy

Cited by 56 publications

References 32 publications

The influence of photodynamic therapy parameters on the inactivation ofCandidaspp:in vitroandin vivostudies

The influence of photodynamic therapy parameters on the inactivation ofCandidaspp:in vitroandin vivostudies

Light based anti-infectives: ultraviolet C irradiation, photodynamic therapy, blue light, and beyond

Streptococcus MutansPhotoinactivation by Combination of Short Exposure of a Broad-Spectrum Visible Light and Low Concentrations of Photosensitizers

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