Effectiveness of non-biodegradable poly(2-hydroxyethyl methacrylate)-based hydrogel particles as a fibroblast growth factor-2 releasing carrier

Takeda, Kahoru; Kitagawa, Haruaki; Tsuboi, Ririko; Kiba, Wakako; Sasaki, Junichi; Hayashi, Mikako; Imazato, Satoshi

doi:10.1016/j.dental.2015.09.007

Cited by 17 publications

(8 citation statements)

References 48 publications

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“…Some carriers that have previously been proposed were collagen (Hu et al 1998, Kikuchi et al 2007, Bezerra Da Silva et al 2008, gelatin Ishimatsu et al 2009), agarose (Chaussain et al 2009), sodium alginate (Oliva-Rodriguez et al 2011), chitosan . Current in vitro evidence suggests other potential drug delivery systems for bioactive molecules, such as biodegradable polymer matrix of lactide and glycolide (Mathieu et al 2013), porous silk fibroin scaffolds (Yang et al 2015), poly-2-hydroxyethyl methacrylate (polyHEMA)-based hydrogel (Takeda et al 2015), tricalcium phosphate microsphere-hydrogel composite (Lee et al 2014). In addition, biomaterials that can be used as scaffolds for VPT have recently been suggested and could enhance proliferation and differentiation of human dental pulp cells, such as polycaprolactone/submicron bioactive glass hybrid composites (Wang et al 2016a), a chitosan-based scaffold (Bellamy et al 2016) and keratin hydrogel (Sharma et al 2016).…”

Section: Current Challenges and Future Directionsmentioning

confidence: 99%

Disclosing the physiology of pulp tissue for vital pulp therapy

2018

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The discovery that dentine is a reservoir of bioactive molecules that can be recruited on demand has attracted efforts to develop new protocols and materials for vital pulp therapy (VPT). The noncollagenous proteins (NCPs) present in the dentine extracellular matrix (ECM) include growth factors (TGF-β1, BMP-7, FGF-2, IGF-1 and IGF-2, NGF and GDNF), extracellular matrix molecules (DSP, DPP, BSP, DMP-1 and DSPP) and both anti-inflammatory and pro-inflammatory chemokines and cytokines (TNF-α, IL-1, IL-6 and IL-10). Molecules such as DSP and DPP are mainly expressed by odontoblasts, and they are cleaved products from dentine sialophosphoprotein (DSPP). Some molecules, such as TGF-β1, specifically interact with decorin/biglycan in dentine. Although TGF-β1 increases the expression and secretion of NGF in human pulp cells, NGF induces mineralization and increases the expression of DSPP and DMP-1. Furthermore, GDNF may act as a cell survival factor and mitogen during tooth injury and repair. Pulp capping materials, such as MTA and calcium hydroxide, can solubilize bioactive dentine molecules (TGF-β1, NGF and GDNF) that stimulate tertiary dentinogenesis. The binding of these signalling molecules leads to activation of several signalling transduction pathways involved in dentinogenesis, odontoblast differentiation and inflammatory responses, such as the p38 MAPK, NF-kβ and Wnt/β-catenin signalling pathways. Understanding the cascade of cellular and molecular events underlying the repair and regeneration processes provides a reasonable new approach to VPT through a targeted interaction between tooth tissue and bioactive molecules.

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Section: Current Challenges and Future Directionsmentioning

confidence: 99%

Disclosing the physiology of pulp tissue for vital pulp therapy

2018

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“…As a non-biodegradable carrier suitable for delivery of proteins or antimicrobials 29,30) , we have developed poly(2-hydroxyethylmethacrylate)/trimethylolpropane trimethacrylate (polyHEMA/TMPT) particles. When FGF-2 was loaded into such particles, a sustained release of FGF-2 was maintained for up to 14 days 28) .…”

Section: Discussionmentioning

confidence: 99%

Influence of polymerization properties of 4-META/MMA-based resin on the activity of fibroblast growth factor-2

et al. 2017

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Dental adhesive resins based on 4-methacryloxyethyl trimellitate anhydride (4-META)/methyl methacrylate (MMA) have been utilized for root-end filling and the bonding of fractured roots. To increase the success rate of these treatments, it would be beneficial to promote the healing of surrounding tissue by applying growth factors. In this study, the influences of the polymerization properties of 4-META/MMA-based resins on the activity of fibroblast growth factor-2 (FGF-2) were evaluated in vitro. The temperature increase caused by the heat generation during polymerization of the 4-META/MMA-based resin was insufficient to change the structure and function of FGF-2. Unpolymerized monomers released from the cured 4-META/MMA-based resin had no negative influences on the ability of FGF-2 to promote the proliferation of osteoblast-like cells. These findings suggest that it is possible to use FGF-2 in combination with 4-META/MMA-based resins.

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“…The non-biodegradable polyHEMA/TMPT particles described before can also act as a carrier for growth factors. Takeda et al 102) reported that fibroblast growth factor-2 (FGF-2) could be loaded into polymer particles composed of 90 (wt)% HEMA and 10 (wt)% TMPT, and FGF-2 adsorbed to the particles was released over 14 days and maintained its activity in increasing the proliferation of osteoblast-like cells. Additionally, when the FGF-2-loaded polymer particles were Each resin disc was placed in a 48-well plate, and MC3T3-E1 cells were seeded in each well at 5×10 3 cells/well and cultured for 3 days.…”

Section: Polymer Particles As a Reservoir Of Antimicrobialsmentioning

confidence: 99%

Cutting-edge filler technologies to release bio-active components for restorative and preventive dentistry

et al. 2020

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Advancements in materials used for restorative and preventive treatment is being directed toward "bio-active" functionality. Incorporation of filler particles that release active components is a popular method to create bio-active materials, and many approaches are available to develop fillers with the ability to release components that provide "bio-protective" or "bio-promoting" properties; e.g. metal/calcium phosphate nanoparticles, multiple ion-releasing glass fillers, and non-biodegradable polymer particles. In this review paper, recent developments in cutting-edge filler technologies to release bio-active components are addressed and summarized according to their usefulness and functions, including control of bacterial infection, tooth strengthening, and promotion of tissue regeneration.

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Effectiveness of non-biodegradable poly(2-hydroxyethyl methacrylate)-based hydrogel particles as a fibroblast growth factor-2 releasing carrier

Cited by 17 publications

References 48 publications

Disclosing the physiology of pulp tissue for vital pulp therapy

Disclosing the physiology of pulp tissue for vital pulp therapy

Influence of polymerization properties of 4-META/MMA-based resin on the activity of fibroblast growth factor-2

Cutting-edge filler technologies to release bio-active components for restorative and preventive dentistry

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