Effectiveness of MicroRNA in Down-Regulation of TGF-β Gene Expression in Digital Flexor Tendons of Chickens: In Vitro and In Vivo Study

Chen, Chuan Hao; Zhou, You Lang; Wu, Ya Fang; Cao, Yi; Gao, Jin Song; Tang, Jin Bo

doi:10.1016/j.jhsa.2009.07.015

Cited by 46 publications

(32 citation statements)

References 40 publications

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“…Transforming growth factor-␤ and platelet-derived growth factor B pathways are targets of miRNA-29. The miRNA-29 family are regulators of the production and deposition of collagens in the heart and tendon (4,22,37). Moreover, in skin fibrosis, cotransfection with premiRNA-29 significantly decreased the relative luciferase activity of the reporter gene, suggesting a direct regulation of collagen by miRNA-29 (10).…”

Section: Discussionmentioning

confidence: 98%

“…Several studies have showed that the miRNA-29 family is involved in fibrosis regulation in diseases such as diabetic nephropathy, myocardial infarction, systemic sclerosis, and chondropathy (4,10,22). These miRNAs already have validated targets in vitro and in vivo to extracellular matrix protein mRNAs of collagen IAI, IAII, IAIII, IIIAI, fibrilin, and elastin in heart and several tissues.…”

Section: Discussionmentioning

confidence: 99%

“…Such changes are not concomitant with expression of genes for fetal reprogramming, such as atrial natriuretic factor (ANF) and pathological CH markers including skeletal ␣-actin (14,23,24). The gene expression of these markers is an important criterion to distinguish if the stimuli are simply trophic (growth fast or normal) or stimuli that are primarily hypertrophic, indicating the beginning of a pathological response (4,5). Akt1 is other critical determinant of intracellular signaling pathway in physiological CH induced by aerobic exercise training, thus the Akt1 pathway also differentiates between physiological and pathological CH (8,17,(23)(24)(25).…”

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confidence: 99%

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MicroRNAs 29 are involved in the improvement of ventricular compliance promoted by aerobic exercise training in rats

Soci

Fernandes

Hashimoto

et al. 2011

Physiological Genomics

162

131

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MiRNAs regulate cardiac development, hypertrophy, and angiogenesis, but their role in cardiac hypertrophy (CH) induced by aerobic training has not previously been studied. Aerobic training promotes physiological CH preserving cardiac function. This study assessed involvement of miRNAs-29 in CH of trained rats. Female Wistar rats (n ϭ 7/group) were randomized into three groups: sedentary (S), training 1 (T1), training 2 (T2). T1: swimming sessions of 60 min/5 days/wk/10 wk. T2: similar to T1 until 8th wk. On the 9th wk rats swam 2ϫ/day, and on the 10th wk 3ϫ/day. MiRNAs analysis was performed by miRNA microarray and confirmed by real-time PCR. We assessed: markers of training, CH by ratio of left ventricle (LV) weight/body wt and cardiomyocytes diameter, pathological markers of CH (ANF, skeletal ␣-actin, ␣/␤-MHC), collagen I and III (COLIAI and COLIIIAI) by real-time PCR, protein collagen by hydroxyproline (OH-proline) concentration, CF and CH by echocardiography. Training improved aerobic capacity and induced CH. MiRNAs-1, 133a, and 133b were downregulated as observed in pathological CH, however, without pathological markers. MiRNA-29c expression increased in T1 (52%) and T2 (123%), correlated with a decrease in COLIAI and COLIIIAI expression in T1 (27%, 38%) and T2 (33%, 48%), respectively. MiRNA-29c was inversely correlated to OH-proline concentration (r ϭ 0.61, P Ͻ 0.05). The E/A ratio increased in T2, indicating improved LV compliance. Thus, these results show that aerobic training increase miR-29 expression and decreased collagen gene expression and concentration in the heart, which is relevant to the improved LV compliance and beneficial cardiac effects, associated with aerobic high performance training. cardiac hypertrophy; collagen; molecular markers; swimming training; physiological cardiac hypertrophy; diastolic function microRNAs (miRNAs) are recognized as a new class of gene expression regulators, consisting of short RNAs, singlestranded, that do not synthesize proteins.MiRNAs are currently potential therapeutic targets and biomarkers in cardiovascular research of various physiological and pathological processes (2,3,(33)(34)(35)(36)(37)40). The action of miRNAs occurs at the posttranscriptional level. MiRNAs negatively regulate the expression of their target genes by coupling to the 3=-untranslated regions (3=-UTR) of mRNA expressed by the target gene (direct modulation) or in the 3=-UTR of mRNA related with the expression of target gene (indirect modulation), which represses its translation into protein (18,19).There are several studies involving miRNAs in pathological cardiac hypertrophy (CH) (2, 3, 29, 35, 36). MiRNAs-1, 133a, and 133b are the best known and highly expressed in heart (36) and already have several targets genes validated, as transcription factors, proteins involved in cellular growth and division, rearrangement of myofibrils and cardiac contractility (34). Carè et al. (2) reported that the decreased expression of miRNA-1 and 133 in vivo and in vitro has a critical role in hypertro...

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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MicroRNAs 29 are involved in the improvement of ventricular compliance promoted by aerobic exercise training in rats

Soci

Fernandes

Hashimoto

et al. 2011

Physiological Genomics

162

131

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“…b) studies of miRNAs in different biological contexts, including reproduction (Lee et al, 2011;Lee et al, 2015), skeletomuscular apparatus (Chen et al, 2009a), bird song physiology (Gunaratne et al, 2011), growth/weight gain , and many others; their comprehensive listing would be beyond the scope of this report.…”

Section: Other Factorsmentioning

confidence: 99%

Literature review of baseline information to support the risk assessment of RNAi‐based GM plants

Pačes

Nič²,

Novotný³

et al. 2017

EFS3

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This report is the outcome of an EFSA procurement aiming at investigating and summarising the state of knowledge on (I) the mode-of-action of dsRNA and miRNA pathways, (II) the potential for nontarget gene regulation by dsRNA-derived siRNAs or miRNAs, (III) the determination of siRNA pools in plant tissues and the importance of individual siRNAs for silencing. The report is based on a comprehensive and systematic literature search, starting with the identification and retrieval of~190,000 publications related to the research area and further filtered down with keywords to produce focused collections used for subsequent screening of titles and abstracts. The report is comprised of an (I) Introduction to the field of small RNAs, (II) a Data and Methodologies section containing strategies used for literature search and study selection, and (III) the Results of the literature review organized according to the three main procurement tasks. The outcome of the first task reviews dsRNA and miRNA pathways in mammals (including humans), birds, fish, arthropods, annelids, molluscs, nematodes, and plants. Eight taxon-dedicated chapters are based on~1,400 cumulative references chosen from~10,000 inspected titles and abstracts. We review conserved and divergent aspects of small RNA pathways and dsRNA responses in animals and plants including structure and function of key proteins as well as four basic mechanisms: genome-encoded posttranscriptional regulations (miRNA), degradation of RNAs by short interfering RNA pools generated from long dsRNA (RNAi), transcriptional silencing, and sequence-independent responses to dsRNA. The outcome of the second task focuses on base pairing between small RNAs and their target RNAs and predictability of biological effects of small RNAs in animals and plants. The outcome of the last task reviews methodology, siRNA pools, and movement of small RNAs in plants. Potential transfer of small RNAs between species and circulating miRNAs in mammals is described in the final chapter.

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“…16 CTGF has also been proposed as an attractive target to decrease scarring following tendon injury. 17 Thus, the reduction of CTGF expression in response to SC injections may represent a potential benefit of this treatment. The other two downregulated genes were ADAMTS-1 (a disintegrin and metalloproteinase with thrombospondin motifs 1, by 2.8 fold, p ¼ 0.010) and TIMP-3 (tissue inhibitor of metalloproteinase 3, by 2.1-fold, p ¼ 0.030).…”

Section: Gene Expression Changes Mediated By Mast Cell Degranulationmentioning

confidence: 99%

Sodium cromolyn reduces expression of CTGF, ADAMTS1, and TIMP3 and modulates post‐injury patellar tendon morphology

Sharma

Abraham

Sampaio

et al. 2010

Journal Orthopaedic Research

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The purpose of this study was to determine whether administration of a mast cell inhibitor (sodium cromolyn, SC) would influence tendon repair and extracellular matrix gene expression following acute injury. CD1 mouse patellar tendons were unilaterally injured and mast cell prevalence was determined. The effect of SC injection on tendon hypercellularity, cross-sectional area, collagen organization, and expression of extracellular matrix-related genes was examined. Mast cell prevalence was markedly increased in injured patellar tendons ( p ¼ 0.009), especially at 8 weeks post-injury ( p ¼ 0.025). SC injection increased collagen organization compared to uninjected animals at 4 weeks and attenuated the development of tendon hypercellularity and tendon thickening post-injury. Expression of CTGF, ADAMTS1, and TIMP3 in injured tendon was reduced in the SC group. SC injections moderated the structural alterations of healing tendon in association with downregulation of several genes associated with tendon fibrosis. This work corroborates previous findings pointing to a role of mast cells in tendon repair. ß

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Effectiveness of MicroRNA in Down-Regulation of TGF-β Gene Expression in Digital Flexor Tendons of Chickens: In Vitro and In Vivo Study

Cited by 46 publications

References 40 publications

MicroRNAs 29 are involved in the improvement of ventricular compliance promoted by aerobic exercise training in rats

MicroRNAs 29 are involved in the improvement of ventricular compliance promoted by aerobic exercise training in rats

Literature review of baseline information to support the risk assessment of RNAi‐based GM plants

Sodium cromolyn reduces expression of CTGF, ADAMTS1, and TIMP3 and modulates post‐injury patellar tendon morphology

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