Effectiveness of Lignin-Removal in Simultaneous Saccharification and Fermentation for Ethanol Production from Napiergrass, Rice Straw, Silvergrass, and Bamboo with Different Lignin-Contents

Yasuda, Masahide; Takeo, Keisuke; Matsumoto, Tomoko; TsutomuShiragami,; Sugamoto, Kazuhiro; Matsushita, Yoh‐ichi; YasuyukiIshii,

doi:10.5772/54194

Cited by 12 publications

(11 citation statements)

References 30 publications

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“…The alkaline pretreatment with NaOH solution led to the highest glucose yield after enzymatic hydrolysis basis on per unit mass of dried raw material (0.245 g/g raw material). The ethanol yield of SSF on alkaline‐pretreated Napier grass obtained in this work (75.4%) was also higher than that from Napier grass pretreated by low‐moisture anhydrous ammonia (LMAA) and 1% NaOH . The dilute acid pretreatment yielded biomass that was relatively difficult to be hydrolyzed in the SSF process, in comparison with those treated by alkaline and two‐stage methods.…”

Section: Resultsmentioning

confidence: 66%

“…The ethanol yield of SSF on alkaline-pretreated Napier grass obtained in this work (75.4%) was also higher than that from Napier grass pretreated by low-moisture anhydrous ammonia (LMAA) 5 and 1% NaOH. 26 The dilute acid pretreatment yielded biomass that was relatively difficult to be hydrolyzed in the SSF process, in comparison with those treated by alkaline and two-stage methods. Results suggested that the removal of lignin from the lignocellulose feedstock favored the hydrolysis by cellulases and also the SSF process on the pretreated biomass.…”

Section: F I G U R E 2 Release Of Reducing Sugars Glucose (Solid Symmentioning

confidence: 99%

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Evaluation of different pretreatments of Napier grass for enzymatic saccharification and ethanol production

Tsai

Lee

Kuan

et al. 2018

Energy Science & Engineering

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Due to its fast‐growing and high carbohydrate content, Napier grass has a great potential to be chosen as a raw material for renewable energy production. The Napier grass after different pretreatments was tested for simultaneous saccharification and fermentation (SSF) with dried yeast (Saccharomyces cerevisiae) and cellulase (CTec2) to produce ethanol. For alkaline pretreatment, the grass was incubated with 10% NaOH at a ratio of 1:20 (w/v) at 90°C for 1 hour. For dilute acid pretreatment, the grass was soaked with 1% H2SO4 at a ratio of 1:10 (w/v) and incubated at 120°C for 1 hour. For the two‐stage pretreatment, the resultant solid from dilute acid pretreatment was further treated with 2% NaOH (1:10 w/v) at 80°C for 6 hours. After enzymatic saccharification at 50°C and pH 5.0 for 96 hours using CTec2, yields of glucose from three pretreated Napier grass samples (10% water‐insoluble solids, WIS) were 75.4 ± 2.9%, 55.4 ± 6.5%, and 76.4 ± 5.0%. Using 10% WIS of alkaline‐pretreated Napier grass biomass as the substrate, the 72‐hour SSF led to an ethanol yield of 86.6 ± 3.4%. On the basis of the dried biomass of Napier grass as the raw material, the ethanol yield could reach 0.143 ± 0.006 (g/g). Napier grass biomass could be effectively treated by alkaline, dilute acid, or two‐stage pretreatment methods to remove non‐cellulosic components to some extent. Alkaline pretreatment was found to be superior to dilute acid and two‐stage pretreatment methods, based on ethanol yields obtained under similar SSF conditions.

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Section: Resultsmentioning

confidence: 66%

Section: F I G U R E 2 Release Of Reducing Sugars Glucose (Solid Symmentioning

confidence: 99%

Evaluation of different pretreatments of Napier grass for enzymatic saccharification and ethanol production

Tsai

Lee

Kuan

et al. 2018

Energy Science & Engineering

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“…However, it was found that the alkali-pretreatment was not a useful method for the biological process of napiergrass with its low lignin-content, because of the retardation of the fermentation rate by the inhibitory materials derived from the alkali-pretreatment and the loss of nutrients for the fermentation [19]. The LMAA-pretreatment did not take place in the retardation of fermentation, as shown in Figure 1 where the time profile of CO 2 evolution per 1 g of hollocellulose were compared among LMAA-pretreated, non-treated, and alkali-pretreated napiergrass.…”

Section: Discussionmentioning

confidence: 99%

“…However, the total ethanol yield was still low (44.2%). Moreover, usual alkali-pretreatment did not operate effectively to enhance the ethanol yield [19]. Therefore, we applied a LMAA pretreatment to enhance the ethanol yield from napiergrass.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of Ethanol Production from Napiergrass (<i>Pennisetum purpureum</i> Schumach) by a Low-Moisture Anhydrous Ammonia Pretreatment

Yasuda¹,

Takeo²,

Nagai³

et al. 2013

JSBS

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Napiegrass (Pennisetum purpureum Schumach) was treated with a low-moisture anhydrous ammonia (LMAA) pretreatment by adding an equal weight of water and keeping it under atmospheric ammonia gas at room temperature for four weeks. After the removal of ammonia and washing with water, a simultaneous saccharification and fermentation (SSF) was conducted for the LMAA-pretreated napiergrass (1.33 g) in a buffer solution (8 mL) using a mixture of a cellulase (80 mg) and a xylanase (53 mg) as well as the cell suspension (0.16 mL) of Saccharomyces cerevisiae. Ethanol and xylose resulted in 91.2% and 62.9% yields, respectively. The SSF process was scaled up using LMAA-pretreated napiergrass (100.0 g) to give ethanol (77.2%) and xylose (52.8%). After the removal of ethanol, the pentose fermentation of the SSF solution (40 mL), which contained 1.00 g of xylose, using cell suspension of Escherichia coli KO11 (70 mL) gave 86.3% yield of ethanol. Total ethanol yield reached 68.9% based on xylan (21.4 wt%) and glucan (39.7 wt%) of the LMAA-pretreated napiergrass.

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“…EH and SSF are affected by chemical and physical properties of substrates including lignin, hemicelluloses and acetyl groups content, as well as cellulose crystallinity [2], polymerization degree, specific surface area [3,4], pore volume [5], particle size [6,7], among others. Several studies show that the lignin content negatively affects the enzymatic digestibility [4,[8][9][10][11][12], possibly acting as a physical barrier to restrict the access of cellulases to cellulose [5,10,13]. However, the way in which lignin protects the polysaccharides from enzymatic attack is not clear to date.…”

Section: Introductionmentioning

confidence: 97%

Fourier transform infrared imaging and microscopy studies of Pinus radiata pulps regarding the simultaneous saccharification and fermentation process

Castillo

Araya

Troncoso

et al. 2015

Analytica Chimica Acta

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Effectiveness of Lignin-Removal in Simultaneous Saccharification and Fermentation for Ethanol Production from Napiergrass, Rice Straw, Silvergrass, and Bamboo with Different Lignin-Contents

Cited by 12 publications

References 30 publications

Evaluation of different pretreatments of Napier grass for enzymatic saccharification and ethanol production

Evaluation of different pretreatments of Napier grass for enzymatic saccharification and ethanol production

Enhancement of Ethanol Production from Napiergrass (<i>Pennisetum purpureum</i> Schumach) by a Low-Moisture Anhydrous Ammonia Pretreatment

Fourier transform infrared imaging and microscopy studies of Pinus radiata pulps regarding the simultaneous saccharification and fermentation process

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Effectiveness of Lignin-Removal in Simultaneous Saccharification and Fermentation for Ethanol Production from Napiergrass, Rice Straw, Silvergrass, and Bamboo with Different Lignin-Contents

Cited by 12 publications

References 30 publications

Evaluation of different pretreatments of Napier grass for enzymatic saccharification and ethanol production

Evaluation of different pretreatments of Napier grass for enzymatic saccharification and ethanol production

Enhancement of Ethanol Production from Napiergrass (&lt;i&gt;Pennisetum purpureum&lt;/i&gt; Schumach) by a Low-Moisture Anhydrous Ammonia Pretreatment

Fourier transform infrared imaging and microscopy studies of Pinus radiata pulps regarding the simultaneous saccharification and fermentation process

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Enhancement of Ethanol Production from Napiergrass (<i>Pennisetum purpureum</i> Schumach) by a Low-Moisture Anhydrous Ammonia Pretreatment