Effectiveness of Mentha piperita Leaf Extracts against Oral Pathogens: An in vitro Study

Abstract

Aim:The study aims to assess the Mentha piperita leaf extract's effectiveness against oral pathogens. Materials and methods:The leaf extract of M. piperita was prepared using cold water method. The three microbial strains, i.e., Streptococcus mutans, Aggregatibacter actinomycetemcomitans, and Candida albicans were used as microbiological materials. Chlorhexidine 0.2% was used as positive control. The digital caliper was used to measure the zone of inhibition to know the antimicrobial activity at 24 and 48 hours. To compare the activity within and between the different microbial strains, one-way analysis of variance (ANOVA) was used. To analyze the data, Statistical Package for the Social Sciences (SPSS) software version of 21.0 was used. The p-value <0.05 was considered as statistically significant.Results: Maximum inhibition zone was seen in both M. piperita extracts and 0.2% chlorhexidine with S. mutans at 24 and 48 hours, followed by A. actinomycetemcomitans, and C. albicans respectively. The statistical analysis ANOVA reveals the statistically significant association of M. piperita extracts with p-value <0.001. The comparison with 0.2% chlorhexidine at 24 hours showed a p-value of <0.04 and at 48 hours, it showed a p-value <0.001, which was statistically significant. Conclusion:The present study concluded that M. piperita showed antimicrobial activity against the oral microorganisms which are causing major less or more severe oral diseases

The Antibacterial Activities of Lavandula angustifolia L., Mentha piperita L., and Ribes nigrum L. against Oral Bacteria, and Their Antioxidant Activities

“…There are three studies of Mentha in literature. Raghavan et al (2018) reported that the inhibition zone was found as 20 mm against Streptococcus mutans. Pramila et al (2012) reported that they were determined 1.10 mm for Staphylococcus sp.…”

Vegetal Compounds as Sources of Prophylactic and Therapeutic Agents in Dentistry

“…In folk medicine, M. piperita oil has been used to reduce gingival inflammation and to stop toothache [120]. Raghavan et al [126] conducted a study in which it was demonstrated the antimicrobial action of M. piperita on oral microorganisms such as Streptococcus mutans, Candida albicans, and Aggregatibacter actinomycetemcomitans that can cause serious oral disease. Following this study, we can consider Mentha piperita as an alternative to the conventional treatment of infections, with its antibiotic resistance being more and more common [126].…”

Antibacterial Activity of Combination of Ethanol Extract of Pepermine Leaves (Mentha piperita L.) and Amikacin Against Klebsiella pneumonia, Staphylococcus aureus, and Escherichia coli

“…The bacteria used were K. pneumoniae, S. aureus, and E. coli. The test was carried out using the disk diffusion method, this method was also used by AL-sum and Al-arfaj, (2013) and also Raghavan et al, (2018).…”

“…Peppermint leaves (Mentha piperita L.) have antifungal activity on Candida albicans, Candida tropicalis, Candida glabrata, Candida parapsilosis, and Candida krusei (Furlan et al, 2010). In addition, peppermint leaves also have antibacterial activity, the diameter of the inhibition zone of distilled water extract of peppermint leaves using the disk diffusion method against the bacteria Streptococcus mutans, and Aggregatibacter actinomycetemcomitans, respectively 20.16 ± 0.36 mm, 18.34 ± 1.09 mm (Raghavan et al, 2018).…”