Effectiveness of human mesenchymal stem cells derived from bone marrow cryopreserved for 23–25years

Shen, Jianliang; Huang, Yanwei; Xu, Song; Zheng, Pei-Hao; Yin, Wu; Cen, Jian; Gong, Lizhong

doi:10.1016/j.cryobiol.2012.01.004

Cited by 23 publications

(11 citation statements)

References 37 publications

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“…We observed a significant decrease in the adhesive properties of the cells stored with temperature fluctuations that mimic long-term biobanking. This also corresponds with the observations of other authors who analyzed the actual storage of MSCs for over 20 years [34]. Such an effect may be associated with the observed necrotic and apoptotic events, triggered in the cells by repeated thermal cycles, as well as with the utilization of a large amount of metabolic sources for recovery processes after the stress accumulated during cryopreservation [35].…”

Section: Discussionsupporting

confidence: 88%

“…A study of the parameters of bone marrow MSCs after 20 years of cryopreservation was able to differentiate them into osteoblasts, adipocytes, and neuronal cells [34]. We were also able to differentiate the cells after cryopreservation with temperature fluctuations into three mesenchymal lineages, which were visually identical to the noncryopreserved control and to the samples with constant cryopreservation temperature conditions.…”

Section: Discussionmentioning

confidence: 71%

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Influence of temperature fluctuations during cryopreservation on vital parameters, differentiation potential, and transgene expression of placental multipotent stromal cells

et al. 2017

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BackgroundSuccessful implementation of rapidly advancing regenerative medicine approaches has led to high demand for readily available cellular suspensions. In particular, multipotent stromal cells (MSCs) of placental origin have shown therapeutic efficiency in the treatment of numerous pathologies of varied etiology. Up to now, cryopreservation is the only effective way to preserve the viability and unique properties of such cells in the long term. However, practical biobanking is often associated with repeated temperature fluctuations or interruption of a cold chain due to various technical, transportation, and stocking events. While biochemical processes are expected to be suspended during cryopreservation, such temperature fluctuations may lead to accumulation of stress as well as to periodic release of water fractions in the samples, possibly leading to damage during long-term storage.MethodsIn this study, we performed a comprehensive analysis of changes in cell survival, vital parameters, and differentiation potential, as well as transgene expression of placental MSCs after temperature fluctuations within the liquid nitrogen steam storage, mimicking long-term preservation in practical biobanking, transportation, and temporal storage.ResultsIt was shown that viability and metabolic parameters of placental MSCs did not significantly differ after temperature fluctuations in the range from –196 °C to –100 °C in less than 20 cycles in comparison to constant temperature storage. However, increasing the temperature range to –80 °C as well as increasing the number of cycles leads to significant lowering of these parameters after thawing. The number of apoptotic changes increases depending on the number of cycles of temperature fluctuations. Besides, adhesive properties of the cells after thawing are significantly compromised in the samples subjected to temperature fluctuations during storage. Differentiation potential of placental MSCs was not compromised after cryopreservation with constant end temperatures or with temperature fluctuations. However, regulation of various genes after cryopreservation procedures significantly varies. Interestingly, transgene expression was not compromised in any of the studied samples.ConclusionsAlterations in structural and functional parameters of placental MSCs after long-term preservation should be considered in practical biobanking due to potential temperature fluctuations in samples. At the same time, differentiation potential and transgene expression are not compromised during studied storage conditions, while variation in gene regulation is observed.Electronic supplementary materialThe online version of this article (doi:10.1186/s13287-017-0512-7) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 71%

Influence of temperature fluctuations during cryopreservation on vital parameters, differentiation potential, and transgene expression of placental multipotent stromal cells

et al. 2017

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“…Marrow is usually cryopreserved in 10% DMSO, and studies have shown that marrow, as well as peripheral blood stem cells and umbilical cord blood, may remain cryopreserved for a long period of time (>10 years) while retaining stem cell/progenitor activities for use in clinical practice . Shen and colleagues demonstrated that marrow preserved for 23 to 25 years was effective for MSC culture production with adequate growth characteristics and adequate amounts of adherent, healthy MSCs . Those authors investigated old frozen marrow specimens as starting material (n = 20) for MSCs, but the controls (n = 20) were not paired from the same donors.…”

Section: Discussionmentioning

confidence: 99%

“…[12][13][14] Shen and colleagues demonstrated that marrow preserved for 23 to 25 years was effective for MSC culture production with adequate growth characteristics and adequate amounts of adherent, healthy MSCs. 15 Those authors investigated old frozen marrow specimens as starting material (n 5 20) for MSCs, but the controls (n 5 20) were not paired from the same donors. This is a limitation that contrasts with our study.…”

Section: Discussionmentioning

confidence: 99%

Impact of starting material (fresh versus cryopreserved marrow) on mesenchymal stem cell culture

et al. 2017

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“…BMSCs are an attractive cell choice because of their ease of production via ex vivo expansion, their ability to self-renew with a high proliferative capacity and their ability for multilineage differentiation [89][90][91]. Furthermore, BMSCs can be transplanted into the body directly from freshly isolated tissues or after culture expansion, through degradable biomaterial scaffolds or decellularized allografts, even as complete 3D tissues for organ replacement [92,93].…”

Section: Bone Marrow Mesenchymal Stem Cellsmentioning

confidence: 99%

Re-Epithelialization: A Key Element in Tracheal Tissue Engineering

Zhang

2015

Regen. Med.

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Trachea-tissue engineering is a thriving new field in regenerative medicine that is reaching maturity and yielding numerous promising results. In view of the crucial role that the epithelium plays in the trachea, re-epithelialization of tracheal substitutes has gradually emerged as the focus of studies in tissue-engineered trachea. Recent progress in our understanding of stem cell biology, growth factor interactions and transplantation immunobiology offer the prospect of optimization of a tissue-engineered tracheal epithelium. In addition, advances in cell culture technology and successful applications of clinical transplantation are opening up new avenues for the construction of a tissue-engineered tracheal epithelium. Therefore, this review summarizes current advances, unresolved obstacles and future directions in the reconstruction of a tissue-engineered tracheal epithelium.

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Effectiveness of human mesenchymal stem cells derived from bone marrow cryopreserved for 23–25years

Cited by 23 publications

References 37 publications

Influence of temperature fluctuations during cryopreservation on vital parameters, differentiation potential, and transgene expression of placental multipotent stromal cells

Influence of temperature fluctuations during cryopreservation on vital parameters, differentiation potential, and transgene expression of placental multipotent stromal cells

Impact of starting material (fresh versus cryopreserved marrow) on mesenchymal stem cell culture

Re-Epithelialization: A Key Element in Tracheal Tissue Engineering

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