Effectiveness of glucose–methanol extender for cryopreservation of Huso huso spermatozoa

Aramli, Mohammad Sadegh; Golshahi, Karim; Nazari, Rajab Mohammad; Aramli, Salim; Banan, Ashkan

doi:10.1016/j.anireprosci.2015.09.005

Cited by 10 publications

(15 citation statements)

References 29 publications

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“…; Aramli et al . ). Sugars have been found to reduce the toxicity of permeable CPTs and to stabilise the liposomal membrane during freezing; otherwise, the post‐thaw viability and motility of sperm are significantly affected during freezing (Fahy et al .…”

Section: Marine Vertebratesmentioning

confidence: 97%

“…), groupers (Lim & Le ) and sturgeon (Aramli et al . ). In addition to sperm, grouper embryos have exhibited promising results when vitrified with either sucrose or trehalose (Tian et al .…”

Section: Marine Vertebratesmentioning

confidence: 97%

“…) and the European sturgeon Huso huso (Aramli et al . ). Certain studies have shown that the use of glucose–MeOH‐based extenders for the cryopreservation of the sperm of rainbow trout by placing samples on a Styrofoam raft above LN 2 produces high motility sperm with a high fertilisation rate: Ciereszko et al .…”

Section: Marine Vertebratesmentioning

confidence: 97%

“…Aramli et al . () compared the use of glucose with sucrose in MeOH‐based extenders in a simple cryopreservation procedure for sturgeon sperm. The results revealed that the simpler extender containing glucose was more effective than the complex extender containing sucrose for freezing (sperm motility: 50% for glucose versus 30% for sucrose).…”

Section: Marine Vertebratesmentioning

confidence: 99%

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Sugars as supplemental cryoprotectants for marine organisms

Tsai

Chong

Meng

et al. 2017

Reviews in Aquaculture

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Cryopreservation has been extensively used in various mega‐industries and has recently been applied in genetic banking for conservation purposes. Compared with conventional cell preservation methods, cryopreservation can maintain the viability of cryopreserved cells for an indefinite time at a relatively lower cost and lesser manpower, with lower probabilities of contamination and genetic changes. This study presents the crucial role of sugar, a cryoprotectant supplement in cryopreservation. Sugar molecules typically interact with the lipid bilayer during the freezing phase to maintain plasma membrane integrity when cells undergo dehydration. When combined with other permeable cryoprotectants such as glucose with methanol and trehalose with dimethyl sulphoxide, sugar prolongs and enhances cellular post‐thaw viability. Moreover, terrestrial and marine organisms have benefited from the inclusion of sugar in the cryopreservation protocol. For wide range of cells such as the sperm, oocytes, embryos and larvae of marine vertebrates and invertebrates, as well as marine algae, cryopreservation with sugar produced positive results compared with cryopreservation without sugar. Not all sugar is beneficial, and the type and concentration of sugar should be applied according to the species. Moreover, the freezing method may also affect the function of sugar. Nevertheless, understanding the role of sugar in cryobiology and conducting a preliminary trial of sugar for cryopreserving cells would benefit future research on cryopreservation.

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Section: Marine Vertebratesmentioning

confidence: 97%

Section: Marine Vertebratesmentioning

confidence: 97%

Section: Marine Vertebratesmentioning

confidence: 97%

Section: Marine Vertebratesmentioning

confidence: 99%

See 2 more Smart Citations

Sugars as supplemental cryoprotectants for marine organisms

Tsai

Chong

Meng

et al. 2017

Reviews in Aquaculture

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“…The genetic resource banking of sperm along with restocking can maintain both the genetic variation and recover viable populations of Persian sturgeon (Aramli et al. ).…”

Section: Introductionmentioning

confidence: 99%

Retracted: Influence of Glutamine Supplementation on Motility and Fertilization Success of Frozen–Thawed Persian Sturgeon (Acipenser persicus) Sperm

Aramli

Golshahi

Nazari³

et al. 2016

Reprod Domestic Animals

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Amino acids have an important biological role for the prevention of cell damage during cryopreservation. The aim of this study was to investigate the effects of glutamine on post-thaw sperm motility and fertilization success in the Persian sturgeon (Acipenser persicus). Sperm collected from six fish was cryopreserved in extenders containing different glutamine concentrations (2.5, 5 and 10 mm). Sperm samples diluted at the ratio of 1 : 1 using the extenders were subjected to cryopreservation. After dilution, the sperm suspensions were sucked into 250-μl straws; the straws were placed on the tray, frozen in nitrogen vapour and plunged into liquid nitrogen. Then, sperm were thawed in a water bath at 40°C for 5 s and used for analysis. Our results revealed that an increase in the concentration of glutamine caused a significant increase in the motility percentage, curvilinear velocity (VCL) and also fertilization success in the Persian sturgeon (p < 0.05). Comparing all concentrations of glutamine, the best concentration for sperm motility and fertilization rate was 10 mm. In addition, higher post-thaw motility percentage, VCL, and fertilization and hatching rates were obtained with the extender at the concentration of 10 mm (p < 0.05). The findings of this study showed that glutamine was of greater benefit to Persian sturgeon sperm motility during frozen-thawed process.

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