Effectiveness of Garlic Bulb (Allium Sativum L.) Ethanol Extract Cream on the Number of Fibroblast Cells in the Healing Process of Male White Rat (Rattus norvegicus L.) Wistar Strain Incision Wound

Teresa, Astrid

doi:10.14704/nq.2020.18.8.nq20199

Cited by 4 publications

(4 citation statements)

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“…We found that oral and topical GEs increased collagen I levels in rats. It has been shown that garlic accelerates wound healing while also increasing fibroblast proliferation and the maturation of collagen bands, and accelerating reepithelialization [26]. Dimethyl sulfone and many other garlic ingredients can increase collagen synthesis by providing a source of sulfur, which is vital for synthesis.…”

Section: Discussionmentioning

confidence: 99%

Garlic Exosomes Promote Hair Growth Through the Wnt/β-catenin Pathway and Growth Factors

Inan Yuksel,

Cicek,

Demir

et al. 2023

Cureus

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BackgroundExosomes are membrane-derived nanovesicles produced by cells and play an important role in intercellular communication. ObjectivesThis study aimed to investigate the effects of garlic exosome (GE) on hair growth. MethodsForty-two Sprague-Dawley/Wistar albino rats were randomly divided into six groups: non-shaved control, shaved control, topical control, GE 2 mg, GE 4 mg, and topical GE. At the end of the experiment, the number of hair follicles, follicle diameter, and subcutaneous tissue thicknesses were measured histopathologically. The Wnt-1, β-catenin, platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1), and collagen I levels were measured by the Western Blot method. ResultsThe anagen follicle counts of the GE 2 mg, 4 mg, and topical GE groups were 66.57±15.49, 105.71±25.06, and 55.29±6.72, and were significantly higher than the control groups (p<0.01, p<0.001 and p<0.05, respectively). The follicle diameter of the GE 4 mg group was higher than the others (p<0.05). The Wnt-1, PDGF, VEGF, TGF-β1, and collagen I levels of all GE groups, and the β-catenin levels of the GE 4 mg and topical GE groups were significantly higher than the control groups (p<0.05). ConclusionGE induces hair growth in rats via the Wnt-1, β-catenin, VEGF, PDGF, and TGF-β1 signaling pathways.

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Section: Discussionmentioning

confidence: 99%

Garlic Exosomes Promote Hair Growth Through the Wnt/β-catenin Pathway and Growth Factors

Inan Yuksel,

Cicek,

Demir

et al. 2023

Cureus

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“…Garlic has several ingredients that play a pivotal role in skin tissue regeneration, namely allicin, ajoene, and DATS [9]. Similarly, a lately published report suggested ethanolic garlic extract was able to attract fibroblasts toward the site of injury significantly, thereby promoting wound healing [33,51]. Since the garlic was extracted in this study using ethanol as a solvent, it is envisaged that either ethanol could extract the constituents in higher quantities or extract some novel compounds, translating into significantly accelerated skin tissue regeneration.…”

Section: In Vivo Wound Healingmentioning

confidence: 99%

“…The ethanolic extract of garlic has been found to attenuate drug-induced nephrotoxicity [30], exert antibacterial activity against antibioticresistant mycobacteria [31], and exert hepatoprotective effects [32]. Moreover, a recently published report suggested that the ethanolic extract of garlic could significantly attract fibroblasts toward the injury site and promote wound healing [33], which is envisaged to promote hastened collagen synthesis and deposition at the wound site, thereby accelerating skin regeneration.…”

Section: Introductionmentioning

confidence: 99%

Chitosan based ethanolic Allium Sativum extract hydrogel film: a novel skin tissue regeneration platform for 2nd degree burn wound healing

Wang,

Ismail,

Khan

et al. 2024

Biomed. Mater.

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This study investigated the potential of ethanolic garlic extract-loaded chitosan hydrogel film for burn wound healing in an animal model. The ethanolic garlic extract was prepared by macerating fresh ground garlic cloves in ethanol for 24 h, followed by filtration and concentration using a rotary evaporator. Hydrogels were then prepared by casting a chitosan solution with garlic extract added at varying concentrations for optimization and, following drying, subjected to various characterization tests, including moisture adsorption (MA), water vapor transmission rate (WVTR), and water vapor permeability rate (WVPR), erosion, swelling, tensile strength, vibrational, and thermal analysis, and surface morphology. The optimized hydrogel (G2) was then analyzed in vivo for its potential for healing 2nd degree burn wounds in rats, and histological examination of skin samples on day 14 of the healing period. Results showed optimized hydrogel (G2; chitosan: 2 g, garlic extract: 1 g) had MA of 56.8% ± 2.7%, WVTR and WVPR of 0.00074 ± 0.0002, and 0.000 498 946 ± 0.0001, eroded up to 11.3% ± 0.05%, 80.7% ± 0.04% of swelling index, and tensile strength of 16.6 ± 0.9 MPa, which could be attributed to the formation of additional linkages between formulation ingredients and garlic extract constituents at OH/NH and C=O, translating into an increase in transition melting temperature and enthalpy (ΔT = 238.83 °C ± 1.2 °C, ΔH = 4.95 ± 0.8 J g−1) of the chitosan moieties compared with blank. Animal testing revealed G2 formulation significantly reduced the wound size within 14 d of the experiment (37.3 ± 6.8–187.5 ± 21.5 mm2) and had significantly higher reepithelization (86.3 ± 6.8–26.8 ± 21.5 and 38.2% ± 15.3%) compared to untreated and blank groups by hastening uniform and compact deposition of collagen fibers at the wound site, cementing developed formulation a promising platform for skin regeneration.

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“…Fibroblasts change their structure into myofibroblasts that can contract the tissue. This happens to help form new tissue in closing the wound (35,36).…”

Section: Figurementioning

confidence: 99%

Enhanced wound healing effect of Areca catechu L. ointment via antibacterial activity and anti-inflammatory process at grade IIA burns in rats

Sandhiutami,

Fahleni,

Miftahurrohmah

et al. 2023

J Herbmed Pharmacol

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Introduction: Areca catechu L. seeds contain flavonoids, tannins, saponins, and alkaloids that have antibacterial properties, can prevent skin infections, and have been used empirically for wound healing. This study aimed to determine the antibacterial activity and effectiveness of A. catechu ointment in wound healing at grade IIA burns in rats. Methods: A. catechu seed extract was formulated into an ointment and then tested for its antibacterial activity using the agar diffusion method. Wound healing testing was conducted by dividing the rats into four groups: negative control, positive control, Formula I (F 1) ointment, and Formula II (F II) ointment. Grade IIA burns were made on the back skin of rats and treatment was performed for 14 days. The wound tissue was taken for histopathological observations. Results: In this study, F II ointment had better antibacterial activity than F I, as indicated by a wider diameter of inhibition against bacteria Pseudomonas aeruginosa, Acinetobacter baumannii, Escherichia coli, Streptococcus pyogenes, and Staphylococcus aureus. The scab formed on F II was faster on day 3, the wound diameter was reduced on day 7, and there was a decrease in inflammatory cell infiltration and coagulative necrosis and an increase in neovascularization and collagen formation on the 7th day (P < 0.05) compared to the negative control and F I. Conclusion: A. catechu seed extract ointment with a concentration of 5.0% (F II) had a better effect on wound healing regarding the antibacterial and anti-inflammatory activity than that with a concentration of 2.5% (F I).

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Effectiveness of Garlic Bulb (Allium Sativum L.) Ethanol Extract Cream on the Number of Fibroblast Cells in the Healing Process of Male White Rat (Rattus norvegicus L.) Wistar Strain Incision Wound

Cited by 4 publications

References 0 publications

Garlic Exosomes Promote Hair Growth Through the Wnt/β-catenin Pathway and Growth Factors

Garlic Exosomes Promote Hair Growth Through the Wnt/β-catenin Pathway and Growth Factors

Chitosan based ethanolic Allium Sativum extract hydrogel film: a novel skin tissue regeneration platform for 2nd degree burn wound healing

Enhanced wound healing effect of Areca catechu L. ointment via antibacterial activity and anti-inflammatory process at grade IIA burns in rats

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