Effectiveness of Four Lytic Phages Against Biofilm-Producing and Multidrug-Resistant Escherichia Coli

Zeid, Azza Abou; Swelim, M. A.; Reda, Fayiz M.; Haveez, A. M. Abd El; Nasr-Eldin, Mohamed A.

doi:10.21608/bjas.2021.167708

Cited by 6 publications

(3 citation statements)

References 52 publications

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“…Single phages usually have a narrow host range as they are generally specific for a limited set of strains of the same bacterial species [ 316 ]. A phage mixture or cocktail is commonly used to target either mono or several bacterial strains due to its greater efficacy in biofilm destruction than a single phage application [ 317 , 318 , 319 ]. The use of phage cocktails arises from the fact that simultaneous treatment targeting a variety of bacterial receptors with diverse antibacterial pathways will more efficiently decrease the bacterial burden, expand host range coverage and lysis potential, and mitigate resistance or development of lysogenic strains [ 316 , 320 ].…”

Section: Qs Pathways Inhibitionmentioning

confidence: 99%

“…Similarly, the phage cocktail composed of four lytic ΦEcp1, ΦEcp2, ΦEcp3, and ΦEcp4 phages completely inhibited the growth of MDR E. coli and significantly prevented the development of biofilms. The phage mixture caused strong biomass reduction of biofilm and showed the highest biofilm inhibition, up to nearly 87% [ 318 ]. Several experiments had more extensive bactericidal results when phage therapy was combined with antibiotics as a single treatment [ 328 , 342 ].…”

Section: Qs Pathways Inhibitionmentioning

confidence: 99%

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Molecular Aspects of the Functioning of Pathogenic Bacteria Biofilm Based on Quorum Sensing (QS) Signal-Response System and Innovative Non-Antibiotic Strategies for Their Elimination

Juszczuk-Kubiak

2024

IJMS

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One of the key mechanisms enabling bacterial cells to create biofilms and regulate crucial life functions in a global and highly synchronized way is a bacterial communication system called quorum sensing (QS). QS is a bacterial cell-to-cell communication process that depends on the bacterial population density and is mediated by small signalling molecules called autoinducers (AIs). In bacteria, QS controls the biofilm formation through the global regulation of gene expression involved in the extracellular polymeric matrix (EPS) synthesis, virulence factor production, stress tolerance and metabolic adaptation. Forming biofilm is one of the crucial mechanisms of bacterial antimicrobial resistance (AMR). A common feature of human pathogens is the ability to form biofilm, which poses a serious medical issue due to their high susceptibility to traditional antibiotics. Because QS is associated with virulence and biofilm formation, there is a belief that inhibition of QS activity called quorum quenching (QQ) may provide alternative therapeutic methods for treating microbial infections. This review summarises recent progress in biofilm research, focusing on the mechanisms by which biofilms, especially those formed by pathogenic bacteria, become resistant to antibiotic treatment. Subsequently, a potential alternative approach to QS inhibition highlighting innovative non-antibiotic strategies to control AMR and biofilm formation of pathogenic bacteria has been discussed.

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Section: Qs Pathways Inhibitionmentioning

confidence: 99%

Section: Qs Pathways Inhibitionmentioning

confidence: 99%

Molecular Aspects of the Functioning of Pathogenic Bacteria Biofilm Based on Quorum Sensing (QS) Signal-Response System and Innovative Non-Antibiotic Strategies for Their Elimination

Juszczuk-Kubiak

2024

IJMS

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“…They also demonstrated that the use of a single phage to the mixed E. coli-S. aureus, instead, promoted the formation of the BF by the alternate strain that was not affected by the phage. A recent study showed that a phage cocktail based on four lytic phages inhibited the growth of MDR E. coli and caused a strong biomass reduction in the BF up to nearly 87% [240,241].…”

Section: Phage Therapymentioning

confidence: 99%

Anti-Biofilm Strategies: A Focused Review on Innovative Approaches

Iaconis,

De Plano,

Caccamo

et al. 2024

Microorganisms

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Biofilm (BF) can give rise to systemic infections, prolonged hospitalization times, and, in the worst case, death. This review aims to provide an overview of recent strategies for the prevention and destruction of pathogenic BFs. First, the main phases of the life cycle of BF and maturation will be described to identify potential targets for anti-BF approaches. Then, an approach acting on bacterial adhesion, quorum sensing (QS), and the extracellular polymeric substance (EPS) matrix will be introduced and discussed. Finally, bacteriophage-mediated strategies will be presented as innovative approaches against BF inhibition/destruction.

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A Review of Challenges and Solutions of Biofilm Formation of Escherichia coli: Conventional and Novel Methods of Prevention and Control

Sadeghzadeh,

Esfandiari,

Khaneghah

et al. 2024

Food Bioprocess Technol

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Effectiveness of Four Lytic Phages Against Biofilm-Producing and Multidrug-Resistant Escherichia Coli

Cited by 6 publications

References 52 publications

Molecular Aspects of the Functioning of Pathogenic Bacteria Biofilm Based on Quorum Sensing (QS) Signal-Response System and Innovative Non-Antibiotic Strategies for Their Elimination

Molecular Aspects of the Functioning of Pathogenic Bacteria Biofilm Based on Quorum Sensing (QS) Signal-Response System and Innovative Non-Antibiotic Strategies for Their Elimination

Anti-Biofilm Strategies: A Focused Review on Innovative Approaches

A Review of Challenges and Solutions of Biofilm Formation of Escherichia coli: Conventional and Novel Methods of Prevention and Control

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