Effectiveness of five antidandruff cosmetic formulations against planktonic cells and biofilms of dermatophytes

Santos, Renan Martins dos; Dias-Souza, Marcus Vinícius

doi:10.1016/j.sjbs.2015.09.033

Cited by 22 publications

(13 citation statements)

References 20 publications

(25 reference statements)

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“…Some studies conducted by our group have already demonstrated the anti-biofilm activity of chalcones against fungal species such as C. gattii ( Palanco et al., 2017 ) and H. capsulatum ( Melo et al., 2017 ). Studies have been carried out to assess the anti-biofilm action of conventional drugs for treating dermatophytoses such as TRB, FLZ ( Costa-Orlandi et al., 2020 ), itraconazole, voriconazole, griseofulvin ( Brilhante et al., 2018 ), econazole ( Toukabri et al., 2018 ), and formulations of piroctone-based shampoos ( Santos; Dias-Souza, 2017 ). However, in most cases, 50 times the MIC is required to observe any biofilm inhibition ( Brilhante et al., 2018 ).…”

Section: Discussionmentioning

confidence: 99%

2-Hydroxychalcone as a Potent Compound and Photosensitizer Against Dermatophyte Biofilms

Bila

Costa-Orlandi

Vaso

et al. 2021

Front. Cell. Infect. Microbiol.

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Dermatophytes, fungi that cause dermatophytosis, can invade keratinized tissues in humans and animals. The biofilm-forming ability of these fungi was described recently, and it may be correlated with the long treatment period and common recurrences of this mycosis. In this study, we evaluated the anti-dermatophytic and anti-biofilm activity of 2-hydroxychalcone (2-chalcone) in the dark and photodynamic therapy (PDT)-mediated and to determine its mechanism of action. Trichophyton rubrum and Trichophyton mentagrophytes strains were used in the study. The antifungal susceptibility test of planktonic cells, early-stage biofilms, and mature biofilms were performed using colorimetric methods. Topographies were visualized by scanning electron microscopy (SEM). Human skin keratinocyte (HaCat) monolayers were also used in the cytotoxicity assays. The mechanisms of action of 2-chalcone in the dark and under photoexcitation were investigated using confocal microscopy and the quantification of ergosterol, reactive oxygen species (ROS), and death induction by apoptosis/necrosis. All strains, in the planktonic form, were inhibited after treatment with 2-chalcone (minimum inhibitory concentration (MIC) = 7.8-15.6 mg/L), terbinafine (TRB) (MIC = 0.008–0.03 mg/L), and fluconazole (FLZ) (1–512 mg/L). Early-stage biofilm and mature biofilms were inhibited by 2-chalcone at concentrations of 15.6 mg/L and 31.2 mg/L in all tested strains. However, mature biofilms were resistant to all the antifungal drugs tested. When planktonic cells and biofilms (early-stage and mature) were treated with 2-chalcone-mediated PDT, the inhibitory concentrations were reduced by four times (2–7.8 mg/L). SEM images of biofilms treated with 2-chalcone showed cell wall collapse, resulting from a probable extravasation of cytoplasmic content. The toxicity of 2-chalcone in HaCat cells showed higher IC50 values in the dark than under photoexcitation. Further, 2-chalcone targets ergosterol in the cell and promotes the generation of ROS, resulting in cell death by apoptosis and necrosis. Overall, 2-chalcone-mediated PDT is a promising and safe drug candidate against dermatophytes, particularly in anti-biofilm treatment.

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Section: Discussionmentioning

confidence: 99%

2-Hydroxychalcone as a Potent Compound and Photosensitizer Against Dermatophyte Biofilms

Bila

Costa-Orlandi

Vaso

et al. 2021

Front. Cell. Infect. Microbiol.

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“…Biofilm formation by T. rubrum NCPF 935 was performed based on previous method with a few modifications [16,17] The test tubes were allowed to rest at room temperature for 1 h. The gently washed using HEPES buffer after which, the biofilms were visualised at ~485 nm excitation/ ~530 nm emission (green) using a Leica TSC SP2…”

Section: Quantification Of T Rubrum Ncpf Biofilm By Safranin Stainingmentioning

confidence: 99%

Griseofulvin solvate solid dispersions with synergistic effect against fungal biofilms

Al-Obaidi

Kowalczyk

Kalgudi

et al. 2019

Colloids and Surfaces B: Biointerfaces

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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“…SA as well as aspirin (acetylsalicylic acid) solutions have shown up to 90% activity against Candida albicans biofilm disruption [20]. A shampoo formulation containing SA (0.5%) in combination with zinc pyrithione (2%) has also been reported to effectively disrupt biofilms formed by Malassezia furfur and C. albicans as well as T. rubrum and T. mentagrophytes [21]. Jasmonic acid is a phytohormone with potent activity against pathogenic fungi affecting plants as well as Candida spp.…”

Section: Fungal Biofilm Inhibitorsmentioning

confidence: 99%

Innovative Physical Approaches for Onychomycosis: Peeling, Lasers and Beyond

Sonthalia¹,

Goldust

2019

Skin Appendage Disord

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Effectiveness of five antidandruff cosmetic formulations against planktonic cells and biofilms of dermatophytes

Cited by 22 publications

References 20 publications

2-Hydroxychalcone as a Potent Compound and Photosensitizer Against Dermatophyte Biofilms

2-Hydroxychalcone as a Potent Compound and Photosensitizer Against Dermatophyte Biofilms

Griseofulvin solvate solid dispersions with synergistic effect against fungal biofilms

Innovative Physical Approaches for Onychomycosis: Peeling, Lasers and Beyond

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