Effectiveness of endophytic-rhizobial seed inoculation of soybean (Glycine max (L.) Merr.) cultivated in irrigated soil

DUBYNSKA, Olena; IUTYNSKA, Halyna; GOLOBORODKO, Stanislav; TYTOVA, Liudmyla

doi:10.5513/jcea01/23.1.3397

Cited by 3 publications

(1 citation statement)

References 25 publications

(28 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Different studies confirm the efficiency of endosphere and rhizosphere bioinoculation on seeds germination (Iutynska et al, 2022), with positive effects on root development and increased dry weight (Chatterjee et al, 2017).…”

Section: In Vivo Germination Promotionmentioning

confidence: 88%

Ecological indicators and biological resources for hydrocarbon rhizoremediation in a protected area

Melzi,

Zecchin,

Gomarasca

et al. 2024

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Spillage from oil refineries, pipelines, and service stations consistently leads to soil, food and groundwater contamination. Bacterial-assisted phytoremediation is a non-invasive and sustainable solution to eliminate or decrease the concentration of xenobiotic contaminants in the environment. In the present study, a protected area interested by a fuel discharge was considered to assess a bioremediation intervention. From the spill point, a plume of contamination flowed South-West into the aquifer, eventually reaching a wetland area. Soils, groundwaters and plants belonging to the species Scirpus sylvaticus (L.) were sampled. In the majority of the soil samples, concentrations of total petroleum hydrocarbons, both C ≤ 12 and C > 12, exceeded legal limits set forth in Directive 2000/60/EC. The analysis of diatom populations, used as ecological indicators, evidenced morphology alterations and the presence of Ulnaria ulna and Ulnaria biceps species, previously detected in hydrocarbon-polluted waters. Tests for phytotoxicity and phytodegradation, carried out in soil mesocosms, planted with Zea mays and Helianthus annuus, demonstrated that both species significantly contributed to the removal of total petroleum hydrocarbons. Removal of C ≤ 12 and C > 12 petroleum hydrocarbons was in the range of 80%–82% for Z. mays and 71%–72% for H. annuus. Microbial communities inhabiting high organic carbon and vegetated soils were more active in hydrocarbon degradation than those inhabiting subsoils, as evidenced by soil slurry experiments. The abundance of functional genes encoding toluene-benzene monooxygenase (tbmD) and alkane hydroxylase (alkB), quantified in environmental samples, confirmed that the plant rhizosphere recruited a microbial community with higher biodegradation capacity. Bacterial strains isolated from the sampling site were able to grow on model hydrocarbons (hexane, hexadecane and o-, m-, p-xylene) as sole carbon and energy sources, indicating that a natural bio-attenuation process was on-going at the site. The bacterial strains isolated from rhizosphere soil, rhizoplane and endosphere showed plant growth promoting traits according to in vitro and in vivo tests on Z. mays and Oryza sativa, allowing to forecast a possible application of bacterial assisted rhizoremediation to recover the protected area.

show abstract

Section: In Vivo Germination Promotionmentioning

confidence: 88%

Ecological indicators and biological resources for hydrocarbon rhizoremediation in a protected area

Melzi,

Zecchin,

Gomarasca

et al. 2024

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

Agroclimatic Factors and Productivity of Different Maturity Groups Soybean Cultivars Under Endophytic-Rhizobial Inoculation in Conditions of Non-Irrigated Agriculture of the Southern Steppe of Ukraine

Goloborodko,

Iutynska,

Tytova

et al. 2024

Biological Systems: Theory and Innovation

View full text Add to dashboard Cite

The article presents the results of studies of weather and climatic conditions during the production processes in the period of formation of the yield of soybean varieties of different maturity under pre-sowing endophytic-rhizobial inoculation of seeds, as well as the functional activity of the rhizosphere microbiota and plant productivity in conditions of non-irrigated agriculture. The average temperature, relative humidity, and rainfall were determined for the interphase periods, which are the main indicators that affect the evapotranspiration, moisture deficit, and moisture coefficient. The experiments were conducted at the experimental field of the Odesa State Agricultural Research Station, located in the southern steppe zone of Ukraine. The soil of the experimental field is a heavy loamy southern chernozem. The moisture availability indicators show that the southern part of the Steppe zone of Odesa region during the growing season of crops (April-September) in 2023 belonged to the semi-arid zone, and in July and September - to the desert. Evapotranspiration and moisture deficit during the growing season of plants of both soybean varieties (ultra-early maturing Diona and mid-season Sviatohor) varied significantly and depended on the average monthly temperature, relative humidity and rainfall. Evapotranspiration reached 1045.1-1244.0 mm, resulting in a moisture deficit of 913.3-1100.4 mm. Due to insufficient rainfall in June, July and August, most crops experienced an increase in moisture deficit. The use of complex thermotolerant and drought-resistant microbial inoculants with stress-protective functions reduced the negative impact of agroclimatic factors on soybean plants and rhizosphere microbiota. The main directions of scientific and economic activity for obtaining consistently high crop yields in the southern part of the Steppe zone are the structuring of sown areas with an optimal share of irrigated land and the use of innovative biological preparations.

show abstract

Complex Inoculation of Soybeans With Nodule Bacteria Bradyrhizobium Japonicum as a Measure to Optimize Symbiotic Nitrogen Fixation

Vorobey,

Kukol,

Pukhtaievych

et al. 2023

View full text Add to dashboard Cite

Objective. Establish effective mixed inoculants for soybeans based on new active strains of Bradyrhizobium japonicum with different intensity and dynamics of nitrogen-fixing activity to improve nodulation processes and functional activity of legume-rhizobia symbiosis. Methods. Microbiological, physiological, statistical, gas chromatography. Legume-rhizobia systems based on Glycine max L. (Merill) soybean, variety Samorodok, were studied using recombinant strains of B. japonicum: B199, B201, Д47, Д48, Д52, Д60, Д63. Monoinoculation of soybean with B. japonicum 634б served as the control. Results. Mixed inoculation of B. japonicum ensured positive ontogenetic dynamics of root nodule formation in soybean. The indicator of the number of root nodules in experimental plants was 1.2–1.9 times higher versus the control plants from Day 18 to 45 of soybean vegetation. When soybeans were inoculated with B. japonicum 634б + B201 and 634б + Д 52, the mass of nodules at Day 45 of vegetation increased by 30.0–35.3 % versus the control and was the highest (1.10–1.15 g/plant) among the studied variants, as a result of the strengthening effect of complex bacterization. A positive synergistic effect of the combined use of strains was obtained — intensification of the nitrogen-fixing activity of soybean root nodules. In the phase of budding/beginning of flowering and full flowering under combined inoculation with active strains of B. japonicum 634б + Д47, 634б + Д52 and 634б + В201, nitrogen fixation of soybean nodules increased by 10.7–25.5 % (at Day 32), by 13.2–30.9 % (at Day 38), and was also 2.3–2.8 times higher in the phase of full flowering (Day 45 of soybean vegetation) versus the similar indicator of control plants. In the phase of full flowering of plants (Day 45 of vegetation), under mixed inoculation with strains of B. japonicum 634б + Д47, 634б + В201, 634б + Д52 and В201 + Д52, the aboveground mass of plants exceeded that of control plants by 8.3–17.2 % variant, which was probably provided by improved conditions of their nitrogen nutrition, due to enhanced assimilation of N2 by root nodules. The most developed root system was in soybeans inoculated with strains B. japonicum 634б and B. japonicum 634б + Д52, B. japonicum 634б + В201 and B. japonicum Д48 + Д52. Conclusion. Symbiotic features of active recombinant strains of B. japonicum and their variability in functional activity at different stages of soybean ontogenesis should be taken into account when creating complex nitrogen-fixing bacterial preparations for soybeans with the effect of microbiological synergy.

show abstract

Effectiveness of endophytic-rhizobial seed inoculation of soybean (Glycine max (L.) Merr.) cultivated in irrigated soil

Cited by 3 publications

References 25 publications

Ecological indicators and biological resources for hydrocarbon rhizoremediation in a protected area

Ecological indicators and biological resources for hydrocarbon rhizoremediation in a protected area

Agroclimatic Factors and Productivity of Different Maturity Groups Soybean Cultivars Under Endophytic-Rhizobial Inoculation in Conditions of Non-Irrigated Agriculture of the Southern Steppe of Ukraine

Complex Inoculation of Soybeans With Nodule Bacteria Bradyrhizobium Japonicum as a Measure to Optimize Symbiotic Nitrogen Fixation

Contact Info

Product

Resources

About