Background and Objective:A Pap smear test is one of the most effective procedures utilized for cervi cal cancer (CC) screening purposes. Nevertheless, many women do not like to do such a test. The present study aimed to monitor the impact of an educational intervention developed based on the expanded Protection Motivation Theory (PMT) on CC screening behavior. Materials and Methods: A quasi-experiment was conducted on 200 women referred to Karaj health centers, Iran. The participants were randomized into experimental and control groups. The data was collected by a Standardized questionnaire including demographic characteristics, the constructs of the PMT, and a self-administered Emotional Social-Support questionnaire (ESS). They filled the questionnaire before, after, and at 6-months follow-up. Eight educational sessions based on the model were held for the experimental group via lecture, group discussions and role play. The data were analyzed by SPSS (Ver. 24) and repeated measures ANOVA and Chi-square test. Results: In the pre-intervention and post-intervention stages, the mean score of perceived susceptibility from 8.07±3.28 to 11.83±1.99, p erceived severity from 12.64±3.46 to 15.19± 2.85, response efficacy from 12.66±5.37 to 16.06±3.6, selfefficacy from 25.94±5.38 to 29.68±4.68, emotional social support from 27.01±7.16 to 31.46±5.76 and intention from 11.01±2.14 to 13.42±1.65 increased and this difference was statistically significant (p<0.05). The mean score of fear from 10.37±2.29 to 5.54±2.76 and response cost from 6.62±2.13 to 3.97 ±1.69 decreased and that was statistically significant (p<0.05). No significant difference was observed in the control group. Besides, the Pap smear test was increased after the educational intervention in the post-test and follow-up in the experimental group (p<0.05). Conclusion: Based on the findings, PMT and ESS can be used as a framework for designing educational programs to promote CC screening behavior.