Effectiveness of Disinfectants Used in Hemodialysis against both Candida orthopsilosis and C. parapsilosis Sensu Stricto Biofilms

Pires, Regina Helena; Silva, Julhiany de Fátima da; Martins, Carlos Henrique Gomes; Almeida, Ana Marisa Fusco; Soares, Christiane Pienna; Mendes-Giannini, María José Soares

doi:10.1128/aac.01308-12

Cited by 20 publications

(11 citation statements)

References 30 publications

(25 reference statements)

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“…Previously, this strain was recovered from the hydraulic system of a haemodialysis center and maintained at −80 • C [22][23][24]. The strain was identified by a PCR-based method with specific primers directed against the secondary alcohol dehydrogenase (SADH) gene [23] and screened for its ability to form biofilm [22,28]. Prior to experiments, the strain was propagated in yeast extract-peptone-dextrose (YPD) medium (1% [wt vol −1 ] yeast extract, 2% [wt vol −1 ] peptone, 2% [wt vol −1 ] dextrose (Difco)) in an orbital shaker (75 r.p.m.)…”

Section: Organismsmentioning

confidence: 99%

Effectiveness of photoelectrocatalysis treatment for the inactivation of Candida parapsilosis sensu stricto in planktonic cultures and biofilms

Pires

Brugnera

Zanoni

et al. 2016

Applied Catalysis A: General

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Photoelectrocatalysis (PEC) has become an attractive technology to disinfect microorganisms from aqueous environments because of its ability to degrade microorganisms without producing any harmful by-products. PEC methodology was applied to inactivate Candida parapsilosis sensu stricto in planktonic and biofilm cells, and its efficiency was compared to that of photolytic and photocatalytic treatment. In the case of planktonic (free-floating) cells, PEC promoted the inactivation of 10 6 CFU mL −1 after 3 min of treatment, and the addition of silver nanoparticles to the Ti/TiO 2 electrodes had virtually no effect on the disinfection period, although it caused a decrease by a factor of 1.5 in the mineralization efficiency. After 60, 10 and 60 min of treatment, PEC methodology reduced 10 6 CFU mL −1 on fungal biofilms and provided 71, 91 and 92% mineralization for biofilm cells performed on polyvinyl chloride (PVC), silicone, and polytetrafluoroethylene (PTFE), respectively. These results indicated that PEC is a promising process for disinfection of water contaminated with C. parapsilosis sensu stricto in both planktonic and biofilm cells.

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Section: Organismsmentioning

confidence: 99%

Effectiveness of photoelectrocatalysis treatment for the inactivation of Candida parapsilosis sensu stricto in planktonic cultures and biofilms

Pires

Brugnera

Zanoni

et al. 2016

Applied Catalysis A: General

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“…Acetic acid in 5% concentration or at a concentration to produce a pH < 3.5 has been shown to be highly effective in direct killing as well as biofilm matrix penetration of various bacteria as well as Candida spp. [4, 18, 19]. SA as well as aspirin (acetylsalicylic acid) solutions have shown up to 90% activity against Candida albicans biofilm disruption [20].…”

Section: Fungal Biofilm Inhibitorsmentioning

confidence: 99%

Innovative Physical Approaches for Onychomycosis: Peeling, Lasers and Beyond

Sonthalia¹,

Goldust

2019

Skin Appendage Disord

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“…biofilms, under the conditions of dialysis systems (which correspond to a poor medium) where a treatment of at least 15 min was needed to obtain ≥ 50% of yeasts reduction. 470 to 930 lg mL À1 could inhibit the biofilm formation and 1.87-3.75 g L À1 could kill biofilm cells (Pires et al, 2013).…”

Section: Hydrogen Peroxide Treatmentmentioning

confidence: 99%

Efficacy of dental unit disinfectants againstCandidaspp. andHartmannella vermiformis

et al. 2014

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Human oral commensal Candida yeasts, as well as environmental free-living amoebae (FLA) such as Hartmannella, are known to be direct or indirect human pathogens. These microorganisms may be isolated from dental unit waterlines (DUWL), because of contamination coming from the tap water and/or a patient's mouth. This study compared the efficacy of commonly used DUWL disinfectants (chlorine, H2 O2 , and Oxygenal 6©) against three species of Candida (C. albicans, C. glabrata, and C. parapsilosis) and one FLA species (H. vermiformis), growing either as single or as mixed biofilms in tap water. Results showed variable efficacies: H2 O2 had no significant activity, while chlorine was effective but only at the highest doses tested, probably not compatible with DUWL uses. Oxygenal 6© was the most efficacious in preventing the growth of yeasts in tap water. However, in the presence of FLA, Oxygenal 6© displayed a reduced antimicrobial activity against sessile C. albicans. In conclusion, none of the tested disinfectants could eradicate yeasts or FLA. Moreover, the antiyeast activity of Oxygenal 6© was reduced in the presence of FLA. Both sessile or planktonic and mixed or single-species conditions should be considered when evaluating the activity of disinfectants for DUWL maintenance. This study also highlighted that FLA should be included in the testing protocols.

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Effectiveness of Disinfectants Used in Hemodialysis against both Candida orthopsilosis and C. parapsilosis Sensu Stricto Biofilms

Cited by 20 publications

References 30 publications

Effectiveness of photoelectrocatalysis treatment for the inactivation of Candida parapsilosis sensu stricto in planktonic cultures and biofilms

Effectiveness of photoelectrocatalysis treatment for the inactivation of Candida parapsilosis sensu stricto in planktonic cultures and biofilms

Innovative Physical Approaches for Onychomycosis: Peeling, Lasers and Beyond

Efficacy of dental unit disinfectants againstCandidaspp. andHartmannella vermiformis

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