Effectiveness of different chemical agents for disinfection of gutta‐percha cones

Abstract

This aim of this study was to evaluate and compare the efficacy of different chemical methods to disinfect gutta-percha cones (GP). Eighty-six size 80 GP cones were used. The cones were contaminated by immersion in saliva and Enterococcus faecalis. Four chemical agents were used: 1% sodium hypochlorite (G1), 2% chlorhexidine gluconate (G2), 10% povidone iodine (G3) and 0.9% saline solution (G4). GP cones were immersed in the solutions for periods of 1 and 10 min. After the disinfection procedure, the cones were incubated in blood heart infusion and the presence of bacterial growth was analysed by turbidity of the medium. In G4, bacterial growth was observed in all specimens; G3 showed growth after immersion for 1 min when contaminated with E. faecalis; G1 showed diverse results after the immersion for 1 min. Meanwhile, G1 and G3 after 10 min, and G2 at both times evaluated did not show bacterial growth. The immersion of GP cones in 2% chlorhexidine gluconate for 1 min was an effective method for GP disinfection, while 10% povidone iodine and 1% sodium hypochlorite needed 10 min of immersion to disinfect the GP.

Correlation between Enterococcus faecalis and Persistent Intraradicular Infection Compared with Primary Intraradicular Infection: A Systematic Review

“…It is reported that 19.4% of gutta-percha cones removed from their packaging contained bacteria on the surface (34); 5.25% sodium hypochlorite and 2% chlorhexidine are the most common disinfectants for gutta-percha cones. Povidone-iodine and peracetic acid have also been studied for rapid disinfection (34)(35)(36)(37).…”

Efectividad de diferentes agentes antimicrobianos en la desinfección de conos de gutapercha

“…Con respecto a la yodopovidona al 10% para Subha y col 14 , este no alcanza efectividad en 5 minutos de desinfección, y según Nabeshima y col 19 , su efectividad como desinfectante es a los 10 minutos de exposición. Resultados similares pudimos obtener, ya que la acción de la clorhexidina al 2% pudo desinfectar todos los conos de gutapercha, no asi la iodopovidona al 10%, estando esta con un tiempo de contacto de 10 minutos, solo pudo desinfectar un 50% de los conos inmersos.…”

“…Sanmarquina 2015; 18(1): [19][20][21][22] G2 (Hipoclorito de sodio al 2,5%), G3 (peróxido de hidrogeno al 3%), G4 (alcohol etílico al 70%) y G5 (yodopovidona al 10%).…”

“…Sanmarquina 2015; 18(1): [19][20][21][22] húmedo o seco, ya que esto provocaría una alteración en la estructura de la gutapercha, es por ello que estos conos deben ser desinfectados por soluciones químicas como: povidona yodada, glutaraldehído, hipoclorito de sodio, peróxido de hidrógeno, clorhexidina, paraformaldehído, alcohol etílico, amonio cuaternario y recientemente se utiliza la irradiación electrónica 5 .…”

Microbiological analysis of absorbent paper points employed by undergraduate students from a dental school in south of Brazil

“…Then, all the endodontic procedures are addressed to create and maintain the aseptic conditions for root canal filling 1 . The persistence of microorganisms is closely linked to the failures of endodontic treatments, and it often occurs due to failures on the root canal preparation, use of instruments and/or materials contaminated and infiltration of bacteria through the saliva 2,3 .…”