Effectiveness of different chemical agents in rapid decontamination of gutta-percha cones

Cardoso, Celso Luíz; Redmerski, Roberta; Bittencourt, Nilza de Lucas Rodrigues; Kotaka, Cinthia Regiane

doi:10.1590/s1517-83822000000100016

Cited by 30 publications

(41 citation statements)

References 8 publications

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“…The results of our study are consistent with those reported in other qualitative microbiological studies (6,33,35). Suchde et al (35) showed the effectiveness of a 1.5% chlorhexidine gluconate detergent solution (Savlon®) in decontaminating gpc in 30 seconds.…”

Section: Discussionsupporting

confidence: 92%

“…Stabholz et al (33), demonstrated that 2% chlorhexidine in an aqueous solution effectively disinfects gpc contaminated with bacteria from the oral flora (Streptococcus mutans and Streptococcus sanguis), intestinal flora (Escherichia coli and Enterococcus faecalis), skin flora (Staphylococcus aureus), and environment (Bacillus subtilis), within 10 min. A qualitative bacteriological study carried out in our laboratory showed that a 2% chlorhexidine digluconate detergent solution eliminated in 1 (6). On the other hand, Siqueira et al (31) reported that a 2% chlorhexidine digluconate solution showed no sporicidal activity after 10 min of contact with gpc contaminated with spores of Bacillus subtilis (ATCC 19659).…”

Section: Discussionmentioning

confidence: 87%

“…Several tests were used to observe the antimicrobial activity of chemical decontaminants of gpc, but there is no consensus for the best method (4)(5)(6)10,12,19,23,29,31,33,35).…”

Section: Introductionmentioning

confidence: 99%

“…However, its effectiveness in decontaminating gpc has been found to differ in some studies (6,14,31,33,35). In order to better investigate this matter, we carried out a quantitative microbiological study to investigate the antimicrobial activity of 2% chlorhexidine digluconate detergent solution, using as an experimental model gpc contaminated with bacterial cells (Staphylococcus aureus, Escherichia coli, Enterococcus faecalis), yeast (Candida albicans), or bacterial spores (Bacillus subtilis).…”

Section: Introductionmentioning

confidence: 99%

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Disinfection of gutta-percha cones with chlorhexidine

Redmerski¹,

Bulla²,

Moreno³

et al. 2007

Braz. J. Microbiol.

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This study investigated the effectiveness of detergent and aqueous solutions of 2% chlorhexidine digluconate in decontaminating gutta-percha cones (gpc) contaminated with bacteria, yeast, or bacterial spores. Guttapercha cones were contaminated with 10 7 -10 8 colony-forming units per milliliter (cfu/ml) of the following test organisms: Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, or Candida albicans. Spores of Bacillus subtilis were also tested. Contaminated gpc were treated with the chlorhexidine solutions for 1, 5, 10, or 15 min. Each cone was then transferred to a tube containing saline and the micoorganisms were recovered after homogenization for cfu determination. Both detergent and aqueous chlorhexidine solutions were effective in eliminating S. aureus, E. faecalis, and C. albicans cells adhered on the surface of gpc within 1 min of exposure. E. coli was eliminated in 5 min with detergent solution. The Bacillus subtilis spores were eliminated by chlorhexidine solutions within 5 min. The results of this study demonstrated that both aqueous and detergent solutions of 2% chlorhexidine digluconate were effective in decontaminating gpc within 5 minutes of exposure.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 87%

“…Several tests were used to observe the antimicrobial activity of chemical decontaminants of gpc, but there is no consensus for the best method (4)(5)(6)10,12,19,23,29,31,33,35).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Disinfection of gutta-percha cones with chlorhexidine

Redmerski¹,

Bulla²,

Moreno³

et al. 2007

Braz. J. Microbiol.

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show abstract

“…1 Owing to the thermoplastic characteristic of gutta-percha cones, they may not be sterilized by the conventional process in which moist or dry heat is used, because this may cause alteration to the gutta-percha structure. 2,3,4,6,8,10 For this reason, previous studies suggested that several different chemical agents could be used for the rapid decontamination of gutta-percha cones in dentistry.…”

Section: Introductionmentioning

confidence: 99%

Effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones

et al. 2011

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The aim of this study was to evaluate the effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones contaminated in vitro with Escherichia coli, Staphylococcus aureus, Streptococcus mutans, Candida albicans and Bacillus subtilus (in spore form). Two hundred and twenty-five gutta-percha cones were contaminated with standardized suspensions of each microorganism and incubated at 37°C for 24 h. The cones were divided into 10 experimental groups (n = 15), according to the microorganism tested and disinfection testing times. The disinfection procedure consisted of immersing each cone in a plastic tube containing the substance. The specimens remained in contact with the substance for 1 or 2.5 minutes. Afterwards, each cone was transferred to a 10% sodium thiosulphate solution (Na 2 S 2 O 3 ) to neutralize the disinfectant. Microbial biofilms adhering to the cones were dispersed by agitation. Aliquots of 0.1 ml of the suspensions obtained were plated on Sabouraud dextrose agar, or brain and heart infusion agar, and incubated at 37°C for 24 h. The results were expressed in colony forming units (CFU/ml) and the data were submitted to the Wilcoxon Signed Rank Test (level of significance at 0.05). A significant reduction was observed, after 1 minute of exposure, in the test solution for C. albicans (p = 0.0190), S. aureus (p = 0.0001), S. mutans (p = 0.0001), B. subtilis (p = 0.0001), and E. coli (p = 0.0001). After 2.5 minutes of exposure, 100% of the microbial inocula were eliminated. It was concluded that the 2% peracetic acid solution was effective against the biofilms of the tested microorganisms on gutta-percha cones at 1 minute of exposure.

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Poly-3-hydroxybutyrate (P3HB) production by bacteria from xylose, glucose and sugarcane bagasse hydrolysate

Silva

Taciro

Ramos

et al. 2004

J IND MICROBIOL BIOTECHNOL

195

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Fifty-five bacterial strains isolated from soil were screened for efficient poly-3-hydroxybutyrate (P3HB) biosynthesis from xylose. Three strains were also evaluated for the utilization of bagasse hydrolysate after different detoxification steps. The results showed that activated charcoal treatment is pivotal to the production of a hydrolysate easy to assimilate. Burkholderia cepacia IPT 048 and B. sacchari IPT 101 were selected for bioreactor studies, in which higher polymer contents and yields from the carbon source were observed with bagasse hydrolysate, compared with the use of analytical grade carbon sources. Polymer contents and yields, respectively, reached 62% and 0.39 g g(-1) with strain IPT 101 and 53% and 0.29 g g(-1) with strain IPT 048. A higher polymer content and yield from the carbon source was observed under P limitation, compared with N limitation, for strain IPT 101. IPT 048 showed similar performances in the presence of either growth-limiting nutrient. In high-cell-density cultures using xylose plus glucose under P limitation, both strains reached about 60 g l(-1) dry biomass, containing 60% P3HB. Polymer productivity and yield from this carbon source reached 0.47 g l(-1) h(-1) and 0.22 g g(-1), respectively.

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Effectiveness of different chemical agents in rapid decontamination of gutta-percha cones

Cited by 30 publications

References 8 publications

Disinfection of gutta-percha cones with chlorhexidine

Disinfection of gutta-percha cones with chlorhexidine

Effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones

Poly-3-hydroxybutyrate (P3HB) production by bacteria from xylose, glucose and sugarcane bagasse hydrolysate

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