Effectiveness of cinnamon (Cinnamomum zeylanicum) bark oil in the prevention of carbon tetrachloride-induced damages on the male reproductive system

Abstract

In this study, it was aimed to investigate the likelihood of detrimental effects of carbon tetrachloride (CCl4 ) on male reproductive system through oxidative stress mechanism and also protective effects of cinnamon bark oil (CBO). For this purpose, 28 healthy male Wistar rats were divided into four groups, seven rats in each. Group 1 received only olive oil daily; group 2 was treated with 100 mg kg(-1) CBO daily; group 3 was treated with only 0.25 ml kg(-1) CCl4 weekly; and group 4 received weekly CCl4 + daily CBO. All administrations were made by intragastric catheter and maintained for 10 weeks. Body and reproductive organ weights, sperm characteristics, testicular oxidative stress markers and testicular apoptosis were examined. CCl4 administration caused significant decreases in body and reproductive organ weights, testicular catalase (CAT) activity, sperm motility and concentration, and significant increases in lipid peroxidation (LPO) level, abnormal sperm rate and apoptotic index along with some histopathological damages compared with the control group. However, significant improvements were observed in absolute weights of testis and epididymis, all sperm quality parameters, LPO level, apoptotic index and testicular histopathological structure following the administration of CCl4 together with CBO when compared to group given CCl4 only. The findings of this study clearly suggest that CBO has protective effect against damages in male reproductive organs and cells induced by CCl4 .

Ameliorating effect of pomegranate juice consumption on carbon tetrachloride-induced sperm damages, lipid peroxidation, and testicular apoptosis

“…Excessive generation of free radicals-induced DNA damage results in increased testicular apoptotic germ cell (Maheshwari et al, 2009). It has been reported that long-term CCl 4 administration causes testicular DNA damage (Khan, 2012;Yüce et al, 2013). The apoptotic cell index of CCl 4 group was found to be significantly higher than the control group in the present study.…”

“…Therefore, disturbances in the synthesis of androgens and proteins can cause negative changes in body weight and especially in the reproductive organ weights (Fernandes et al, 2007). It has been reported that CCl 4 administration results in reduced body and testis weights (Castilla-Cortazar et al, 2004;Manjrekar et al, 2008;Khan and Ahmed, 2009;Yüce et al, 2013) and decreased testosterone level (Khan, 2012;Khan and Ahmed, 2009). In the present study, CCl 4 caused significant decreases in body weight and also weights of testes, epididymides, and accessory sex glands.…”

“…Free radicals have high affinity to cell membrane lipids, especially PUFAs, leading to tissue damage due to the LPO Türk et al 7 (Aitken and Roman, 2008;Turner and Lysiak, 2008). It has been reported that CCl 4 causes increases in MDA or TBARs levels, which are by-products of LPO (Abraham et al, 1999;Castilla-Cortazar et al, 2004;Fadhel and Amran, 2002;Khan, 2012;Khan and Ahmed, 2009;Soliman and Fahmy, 2011;Yüce et al, 2013) and decreases in enzymatic and nonenzymatic antioxidants in testicular tissue (Khan, 2012;Khan and Ahmed, 2009;Soliman and Fahmy, 2011). CCl 4 administration caused significant increase in testicular MDA level and significant decrease in testicular CAT activity and also nonsignificant decrease in rGSH level and GSH-Px activity in this study.…”

Quercetin attenuates carbon tetrachloride-induced testicular damage in rats

“…In addition, as the testis, prostate (Jiang et al ., ), epididymis (Hudson et al ., ) and germ cells (Liu et al ., ) contain CYP isozymes, it is possible that CCl 4 causes oxidative damage in lipids of these tissues and cells (Abraham et al ., ). Acute or chronic CCl 4 administration has been reported to cause morphological, structural and functional damages in reproductive system through oxidative toxicity in male laboratory animals (Kalla & Bansal, ; Horn et al ., ; Manjrekar et al ., ; Khan & Ahmed, ; Abdou et al ., ; Khan, ; Yüce et al ., ).…”

Safety assessment of potential food ingredients in canine hepatocytes

“…CBO is an antioxidant and may have a protective effect through an oxidative stresss mechanism on the male reproductive system (Yüce et al, 2014). CINA is a major constituent of CBO (~70%) which is an active antifungal compound (Singh et al, 1995) that can inhibit NF-kB through signal tansduction pathways NIK/IKK, ERK, and p38 MAPK, thereby showing good antioxidant properties (Kim et al, 2007).…”