Background Breastfeeding is considered the biological norm and essential to reduce infant morbidity and mortality. Mothers are responsible for breastfeeding but the support of others, including their partners is an influential determinant. The aim of this study was to compare and contrast antenatal breastfeeding knowledge, attitudes, and perceptions of support of expectant couples in Mekelle, Ethiopia. Methods As part of a randomised control trial of an mHealth intervention, 128 couples in their third trimester from three selected health centres in Mekelle city were recruited to participate. Couples who each had a personal mobile phone, read and spoke Tigrigna, and lived together were included. Baseline data on breastfeeding knowledge, attitudes, and perceived support (breastfeeding, savvy, help, appreciation, presence, and responsiveness) were collected using previously validated tools through interview by health workers. Result Compared to mothers, male partners were more likely to indicate their intention to provide breastfeeding appreciation (p=0.02), breastfeeding presence (p=0.002), and breastfeeding responsiveness (p=0.04). The prenatal perception mothers had of their partners’ intended breastfeeding support was lower than fathers’ perceptions to support their partners. Multiparous mothers had more positive perceptions regarding their partners’ breastfeeding savvy (p=0.03), and breastfeeding help (p=0.02) compared to primiparous mothers. Conclusion Fathers in Ethiopia are potentially strong supporters of breastfeeding practice. Future breastfeeding interventions should promote the involvement of fathers in breastfeeding and encourage mothers to increase their partners’ involvement in breastfeeding.