Effectiveness of bacteriophages incorporated in gelatine films against Staphylococcus aureus

Weng, Shihan; Camacho, Ana María; Sáez‐Orviz, Sara; Marcet, Ismael; Garcı́a, Pilar; Rendueles, Manuel; Dı́az, Mario

doi:10.1016/j.foodcont.2020.107666

Cited by 34 publications

(36 citation statements)

References 35 publications

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“…Cellulose acetate films containing bacteriophages have also shown antimicrobial activity against Salmonella Typhimurium [10]. On the other side, gelatin edible films incorporating bacteriophages against Staphylococcus aureus were tested in cheese; however, the best performance was obtained through direct immersion into film forming solution containing bacteriophages [11].…”

Section: Introductionmentioning

confidence: 99%

“…In this work, three biodegradable films of sodium caseinate (SC), sodium alginate with gelatin (SAG), and polyvinyl alcohol (PVOH) were considered for preparing active films as carriers of Listex P100. It must be contemplated that the properties of these films can be modified by introducing bioactive compounds in their formulation and they can be easily applied as coating over other polymeric substrates [11]. To date, there are hardly any studies on the development of active films containing Listex P100 phage owing to the decrease in the growth of L. monocytogenes.…”

Section: Introductionmentioning

confidence: 99%

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Development of Biodegradable Films Loaded with Phages with Antilisterial Properties

et al. 2021

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The inhibitory and bactericidal capacity of Listex P100 bacteriophage has been studied against different concentrations of Listeria monocytogenes in stationary and exponential phases. Three different matrices were employed to developed films incorporating Listex P100: (1) sodium caseinate, (2) sodium alginate mixed with gelatin, and (3) polyvinyl alcohol (PVOH). All the films were successfully developed by casting at room temperature. These active biodegradable films were optical, structural, and thermally characterized, and their antimicrobial capacities against L. monocytogenes were studied. The incorporation of phages did not affect the morphology, colour, opacity, and thermal stability of polymers. The antimicrobial analysis revealed the bacteriophage presented a high antimicrobial capacity against L. monocytogenes in the stationary phase (4.40 and 6.19 log reduction values or bactericide effect depending on the initial inoculum of the pathogen). Developed films showed antimicrobial capacity close to 1 log after 24 h of incubation at 30 °C. The effectiveness of PVOH films was greater under refrigeration conditions, reaching 2 log reduction after eight days of incubation. The use of these films as a coating in a food or as part of a packaging could improve food safety against the growth of pathogenic microorganisms such as Listeria monocytogenes.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of Biodegradable Films Loaded with Phages with Antilisterial Properties

et al. 2021

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“…However, the lack of antibacterial activity of gelatin film is a limitation for its food application as a protective film to improve food safety during storage (Milani & Tirgarian, 2020;Stevenson et al, 2020). Up to now, the potential biocontrol application of packaging films with diverse lytic phages has been demonstrated, such as Salmonella phage φ135, E. coli phage vB_EcoS-EC4 and Pseudomonas fluorescens Phage ϕIBB-PF7A in sodium alginate film (Alves et al, 2019(Alves et al, , 2020, E. coli O157:H7 phage in chitosan film (Cui et al, 2017), Coliphage T4 in whey protein film (Vonasek et al, 2014) and S. aureus phage phiIPLA-RODI in gelatin film (Weng et al, 2021). Their high specificity without affecting the beneficial bacteria responsible for organoleptic properties of food products indicates these lytic phages hold significant promise for improving food safety in food packaging (Cooper, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…coli O157:H7 phage in chitosan film (Cui et al ., 2017), Coliphage T4 in whey protein film (Vonasek et al ., 2014) and S . aureus phage phiIPLA‐RODI in gelatin film (Weng et al ., 2021). Their high specificity without affecting the beneficial bacteria responsible for organoleptic properties of food products indicates these lytic phages hold significant promise for improving food safety in food packaging (Cooper, 2016).…”

Section: Introductionmentioning

confidence: 99%

Effectiveness of bacteriophage JN01 incorporated in gelatin film with protocatechuic acid on biocontrol of Escherichia coli O157:H7 in beef

Zhong

Zhang

et al. 2022

Int J of Food Sci Tech

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Summary A new gelatin‐protocatechuic acid (PCA) film with Escherichia coli O157:H7 phage JN01 was developed and characterised. After incorporated with JN01, swelling value, water vapour permeability, water solubility and elongation at break of gelatin‐PCA film were not significantly different. The addition of JN01 increased b value and transparency of film, while it decreased L value, a value and tensile strength of film. Moreover, the gelatin‐PCA‐JN01 film presented antioxidant activity of 60.07%. Furthermore, JN01 could be steadily released from gelatin‐JN01 and gelatin‐PCA‐JN01 films in aqueous solution, and their release rates were 7.56% and 0.12% after 11 h, respectively. The microstructure analysis showed that JN01 particles were clustered and uniformly distributed in film, and the aggregation would be attenuated in the presence of PCA. Meanwhile, E. coli O157:H7 counts were 1.13 log10CFU mL−1 and 0.45 log10CFU mL−1 lower in gelatin‐JN01 and gelatin‐PCA‐JN01 films compared with pure gelatin film in vitro at 4 °C for 24 h, respectively. After stored at 4 °C for 7 days, Escherichia coli O157:H7 counts were 1.00 log10CFU g−1 and 0.80 log10CFU g−1 lower in beef packed with these two gelatin films, compared with beef without gelatin film, respectively. In conclusion, the developed gelatin‐PCA‐JN01 film has potential application in food preservation.

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“…Among the materials studied, gelatin stands out for being abundant and low cost, in addition to presenting promising lm-forming properties [6] . In addition to having a high degree of biocompatibility and biodegradability, it is widely used in the food industry as a gelling agent and as a stabilizing ingredient for foams and emulsions [7] . It is the product of the denaturation and partial hydrolysis of collagen, composed mainly of carbon (50.5%), oxygen (25.2%), nitrogen (17.0%), and hydrogen (6.8%) [8;9] .…”

Section: Introductionmentioning

confidence: 99%

Transglutaminase effect on the gelatin-films properties

et al. 2021

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BACKGROUND: The development of biodegradable materials, especially those from renewable sources, is important to reduce the impact of plastic waste on the environment. On the other hand, protein-based lms have high solubility in water, in addition to limited mechanical properties, which makes their application in high humidity environments a challenge. The enzyme transglutaminase (TGase) can reduce the interaction of gelatin lms with water, improving these properties, while improving the mechanical properties.Therefore, this study aimed to determine the best condition for using the TGase enzyme, through variations in the gelatin and enzyme mass, in addition to the time that the enzyme acts on the gelatin, evaluating the properties tensile strength, elongation, and solubility in water. In a second set of experiments gelatin amount was kept xed at 4%, and other proportions of an enzyme (1% and 5% w/w gelatin) were studied, evaluating beyond these properties the degradation in simulated soil through thermogravimetry analysis and Fourrier Transform Infrared Spectrometer (FTIR).RESULTS: It was concluded that the higher concentration of TGase (5%) promoted a greater reduction in the solubility of the lms, making the lms more resistant to biodegradation, facilitating their application due to the increase in their useful life. CONCLUSION: At the end of the degradation test, it was noticed that the lms were degraded, presenting the potential for substitution of polymers of fossil origin, which could be an alternative to the problem of polymeric residues in the environment. HighlightsThe addition of the enzyme decreased the solubility of the lms;Films with the enzyme have increased resistance to biodegradation when compared to lms without the enzyme.Gelatin lms with and without enzyme have been completely degraded when expose to soil.

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Effectiveness of bacteriophages incorporated in gelatine films against Staphylococcus aureus

Cited by 34 publications

References 35 publications

Development of Biodegradable Films Loaded with Phages with Antilisterial Properties

Development of Biodegradable Films Loaded with Phages with Antilisterial Properties

Effectiveness of bacteriophage JN01 incorporated in gelatin film with protocatechuic acid on biocontrol of Escherichia coli O157:H7 in beef

Transglutaminase effect on the gelatin-films properties

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