Effectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis

Marques, Fernanda A.; Soares, Rodrigo Pedro; Almeida, Gregório Guilherme; Souza, Cláudio Leite de; Melo, Maria Norma; Pinto, Sebastião Alves; Quixabeira, Valéria Bernadete Leite; Pereira, Ledice I. A.; Dorta, Miriam Leandro; Ribeiro‐Dias, Fátima; Sílveira, Fernando Tobias; Silva, Sydnei M.; Gontijo, Célia Maria Ferreira; Tafuri, Wagner Luiz

doi:10.1016/j.parint.2016.10.003

Cited by 11 publications

(12 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Microscopic analysis confirmed previous observations for dermotropic Leishmania species ( Alves et al, 2013 ; Marques et al, 2017 ). L88-infected animals displayed an intense and diffuse chronic inflammatory process.…”

Section: Resultssupporting

confidence: 87%

“…This is often observed for dermotropic species including Leishmania guyanensis , Leishmania braziliensis , Leishmania amazonensis , and Leishmania shawi . However, in L. amazonensis , the exudate is characterized by vacuolized macrophages intensely parasitized by amastigotes with rare lymphocytes named Virchow granulomas ( de Magalhães et al, 1986 ; Silveira et al, 2004 ; Marques et al, 2017 ). Similarly, CL lesions caused by L. enriettii are also characterized by a chronic inflammatory infiltrate of mononuclear cells.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Salivary Gland Extract Modulates the Infection of Two Leishmania enriettii Strains by Interfering With Macrophage Differentiation in the Model of Cavia porcellus

et al. 2018

Self Cite

View full text Add to dashboard Cite

The subgenus Mundinia includes several Leishmania species that have human and veterinary importance. One of those members, Leishmania Mundinia enriettii was isolated from the guinea pig Cavia porcellus in the 1940s. Several histopathological studies have already been performed in this species in the absence of salivary gland extract (SGE), which are determinant and the early and future events of the infection. Our main hypothesis is that SGE could differentially modulate the course of the lesion and macrophage differentiation caused by avirulent and virulent L. enriettii strains. Here, the C. porcellus nasal region was infected using needles with two strains of L. enriettii (L88 and Cobaia) in the presence/absence of SGE and followed for 12 weeks. Those strains vary in terms of virulence, and their histopathological development was characterized. Some L88-infected animals could develop ulcerated/nodular lesions, whereas Cobaia strain developed non-ulcerated nodular lesions. Animals experimentally inoculated developed a protuberance and/or lesion after the 4th and 5th weeks of infection. Macroscopically, the size of lesion in L88-infected animals was smaller in the presence of SGE. Remarkable differences were detected microscopically in the presence of SGE for both strains. After the 6th and 7th weeks, L88-infected animals were heavily parasitized with an intense inflammatory profile bearing amastigotes and pro-inflammatory cells compared to those infected by Cobaia strain. Morphometry analysis revealed that L1+ macrophages were abundant in the L88 infection, but not in the Cobaia infection. In the presence of SGE, an increased CD163+ macrophage infiltrate by both strains was detected. Interestingly, this effect was more pronounced in Cobaia-infected animals. This study showed the role of SGE during the course of L. enriettii (strains L88 and Cobaia) infection and its role in modulating macrophage attraction to the lesion site. SGE decreased L1+ macrophages and this may favor an escaping mechanism for L88 parasites. On the other hand, in the presence of SGE, an increase in CD163+ cells during Cobaia infection may be important for its control. Although both strains healed at the end of the infection, the role of SGE was determinant for the kinetics of the immunopathological events in this dermotropic species.

show abstract

Section: Resultssupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Salivary Gland Extract Modulates the Infection of Two Leishmania enriettii Strains by Interfering With Macrophage Differentiation in the Model of Cavia porcellus

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…Traditional diagnostic methods, such as direct visualization of amastigotes in smears and biopsies and parasite culture have shown a low sensitivity, which limits their effectiveness in cases with a low parasite burden. 5,6 In recent years, immunohistochemical techniques [7][8][9][10] and, particularly, molecular tests targeting sequences of Leishmania DNA have been developed, showing a high diagnostic sensitivity. 5,9,11,12 We report hereby a large series of cutaneous leishmaniasis, including cases from the outbreak in Madrid and sporadic cases from the preceding and the following years.…”

Section: Introductionmentioning

confidence: 99%

Cutaneous leishmaniasis: A pathological study of 360 cases with special emphasis on the contribution of immunohistochemistry and polymerase chain reaction to diagnosis

et al. 2020

View full text Add to dashboard Cite

Background: Traditional methods for the diagnosis of leishmaniasis yield poor sensitivity, which limits its effectiveness in lesions with a low parasite burden. Methods: Retrospective pathologic study of 360 cases of cutaneous leishmaniasis and analysis of the different diagnostic methods used. Results: In 93% of the lesions, histopathology showed a dense and diffuse inflammatory infiltrate, consisting of lymphocytes, histiocytes and plasma cells, which occupied the superficial and mid dermis and variably extended to deep dermis and superficial subcutis (standard pattern). The remaining cases exhibited atypical features, such as perivascular, interstitial or perifollicular inflammatory patterns, folliculitis or panniculitis. Granulomas were identified in 84% of biopsies, most of them as small, poorly formed, non-necrotizing histiocytic aggregates. Amastigotes were visualized by routine histopathologic exam in 36% of biopsies. Immunohistochemistry stained 17 of 26 lesions (65%) negative by conventional stains. PCR provided the correct diagnosis in 218 cases (58% of the series) negative for Leishmania by other techniques. Conclusions: Biopsies negative for Leishmania by traditional diagnostic methods that show the histopathologic standard pattern, those with atypical features from patients with clinical suspicion of cutaneous leishmaniasis in endemic areas, should be studied by immunohistochemistry and/or PCR for Leishmania in order to reach the definitive diagnosis.

show abstract

“…Leishmania amazonensis is the causative agent of localized cutaneous leishmaniasis (LCL) and anergic diffuse cutaneous leishmaniasis (ADCL). This species, found in the Amazon basin, is transmitted by Lutzomyia flaviscutellata (Diptera: Psychodidae), widely distributed in South America [ 1 , 2 ].…”

Section: Introductionmentioning

confidence: 99%

Lipophosphoglycan polymorphisms do not affect Leishmania amazonensis development in the permissive vectors Lutzomyia migonei and Lutzomyia longipalpis

et al. 2017

Self Cite

View full text Add to dashboard Cite

BackgroundLipophosphoglycan (LPG) is a dominant surface molecule of Leishmania promastigotes. Its species-specific polymorphisms are found mainly in the sugars that branch off the conserved Gal(β1,4)Man(α1)-PO4 backbone of repeat units. Leishmania amazonensis is one of the most important species causing human cutaneous leishmaniasis in the New World. Here, we describe LPG intraspecific polymorphisms in two Le. amazonensis reference strains and their role during the development in three sand fly species.ResultsStrains isolated from Lutzomyia flaviscutellata (PH8) and from a human patient (Josefa) displayed structural polymorphism in the LPG repeat units, possessing side chains with 1 and 2 β-glucose or 1 to 3 β-galactose, respectively. Both strains successfully infected permissive vectors Lutzomyia longipalpis and Lutzomyia migonei and could colonize their stomodeal valve and differentiate into metacyclic forms. Despite bearing terminal galactose residues on LPG, Josefa could not sustain infection in the restrictive vector Phlebotomus papatasi.ConclusionsLPG polymorphisms did not affect the ability of Le. amazonensis to develop late-stage infections in permissive vectors. However, the non-establishment of infection in Ph. papatasi by Josefa strain suggested other LPG-independent factors in this restrictive vector.

show abstract

Effectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis

Cited by 11 publications

References 20 publications

Salivary Gland Extract Modulates the Infection of Two Leishmania enriettii Strains by Interfering With Macrophage Differentiation in the Model of Cavia porcellus

Salivary Gland Extract Modulates the Infection of Two Leishmania enriettii Strains by Interfering With Macrophage Differentiation in the Model of Cavia porcellus

Cutaneous leishmaniasis: A pathological study of 360 cases with special emphasis on the contribution of immunohistochemistry and polymerase chain reaction to diagnosis

Lipophosphoglycan polymorphisms do not affect Leishmania amazonensis development in the permissive vectors Lutzomyia migonei and Lutzomyia longipalpis

Contact Info

Product

Resources

About