Effective vectors for transformation, expression of heterologous genes, and assaying transposon excision in transgenic plants

Jones, Jonathan D. G.; Shlumukov, L. R.; Carland, Francine M.; English, James J.; Scofield, Steven R.; Bishop, Gerard J.; Harrison, Kate

doi:10.1007/bf02525170

Cited by 287 publications

(227 citation statements)

References 41 publications

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“…High molecular weight BAC DNA was purified by cesium chloride-ethidium bromide gradient centrifugation and was partially digested with Sau3AI, dephosphorylated, and size-fractionated on 0.8% low melting agarose gels. Fragments of approximately 15-20 kb were eluted from gels and inserted into the BamHI site of cosmid vector pSLJ755I5 (15). Ligated DNA was packaged in phage heads by using commercial extracts (Stratagene Gigapack III Gold) and used to transfect DH10B Escherichia coli cells (GIBCO).…”

Section: Methodsmentioning

confidence: 99%

Cloning of the Arabidopsis RTM1 gene, which controls restriction of long-distance movement of tobacco etch virus

Chisholm

Mahajan

Whitham

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

191

102

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The locus RTM1 is necessary for restriction of long-distance movement of tobacco etch virus in Arabidopsis thaliana without causing a hypersensitive response or inducing systemic acquired resistance. The RTM1 gene was isolated by map-based cloning. The deduced gene product is similar to the ␣-chain of the Artocarpus integrifolia lectin, jacalin, and to several proteins that contain multiple repeats of a jacalin-like sequence. These proteins comprise a family with members containing modular organizations of one or more jacalin repeat units and are implicated in defense against viruses, fungi, and insects.resistance

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Section: Methodsmentioning

confidence: 99%

Cloning of the Arabidopsis RTM1 gene, which controls restriction of long-distance movement of tobacco etch virus

Chisholm

Mahajan

Whitham

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

191

102

View full text Add to dashboard Cite

show abstract

“…After digesting the DNA partially with Mbol, the ends of the fragments were incompletely filled in with dGTP and dATP. DNA fragments were then fractionated by centrifugation in a linear 1.25 to 5.0 M NaCl gradient, and DNA in the 20-kb range was ligated into the Xhol site (incompletely filled in with dTTP and dCTP) of the cosmid pDCL04541 (Jones et al, 1992). The ligation mix was packaged using Gigapack II (Stratagene, La Jolla, CA) and used to infect the Escherichia coli DH5a.…”

Section: Gene Cloningmentioning

confidence: 99%

The Arabidopsis ABSCISIC ACID-INSENSITIVE2 (ABI2) and ABI1 genes encode homologous protein phosphatases 2C involved in abscisic acid signal transduction.

1997

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Abscisic acid (ABA) mediates seed maturation and adaptive responses t o environmental stress. In Arabidopsis, the ABA-INSENSITIVEl (ABll) protein phosphatase 2C is required for proper ABA responsiveness both in seeds and in vegetative tissues. To determine whether the lack of recessive alleles at the corresponding locus could be explained by the existence of redundant genes, we initiated a search for ABl7 homologs. One such homolog turned out t o be the A612 locus, whose abi2-7 mutation was previously known t o decrease ABA sensitivity. Whereas abi7-7 is (semi)dominant, abi2-7 has been described as recessive and maternally controlled at the germination stage. Unexpectedly, the sequence of the abi2-7 mutation showed that it converts Gly-168 t o Asp, which is precisely the same amino acid substitution found in abi7-7 and at the coincidental position within the A B l l phosphatase domain (Gly-180 t o Asp). In vitro assays and functional complementation studies in yeast confirmed that the AB12 protein is an active protein phosphatase 2C and that the abi2-7 mutation reduced phosphatase activity as well as affinity t o Mg2+. Although a number of differences between the two mutants in adaptive responses t o stress have been reported, quantitative comparisons of other major phenotypes showed that the effects of both abi7-7 and abi2-7 on these processes are nearly indistinguishable. Thus, the homologous ABll and AB12 phosphatases appear t o assume partially redundant functions in ABA signaling, which may provide a mechanism t o maintain informational homeostasis.

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“…The TAMU bacterial artificial chromosome (BAC) T31A11 was digested partially with HindIII and subcloned into the binary cosmid vector pSLJ755I5 (Jones et al, 1992). Partially digested products were size selected by electrophoresis in an 0.8% agarose gel, and fragments longer than 15 kb were recovered by using Gene Clean III (Bio 101, Vista, CA).…”

Section: Cosmid Subclones and Arabidopsis Transformationmentioning

confidence: 99%

Arabidopsis RTM2 Gene Is Necessary for Specific Restriction of Tobacco Etch Virus and Encodes an Unusual Small Heat Shock–like Protein

et al. 2000

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Arabidopsis plants have a system to specifically restrict the long-distance movement of tobacco etch potyvirus (TEV) without involving either hypersensitive cell death or systemic acquired resistance. At least two dominant genes, RTM1 and RTM2 , are necessary for this restriction. Through a series of coinfection experiments with heterologous viruses, the RTM1/RTM2-mediated restriction was shown to be highly specific for TEV. The RTM2 gene was isolated by a mapbased cloning strategy. Isolation of RTM2 was confirmed by transgenic complementation and sequence analysis of wild-type and mutant alleles. The RTM2 gene product is a multidomain protein containing an N-terminal region with high similarity to plant small heat shock proteins (HSPs). Phylogenetic analysis revealed that the RTM2 small HSP-like domain is evolutionarily distinct from each of the five known classes of plant small HSPs. Unlike most other plant genes encoding small HSPs, expression of the RTM2 gene was not induced by high temperature and did not contribute to thermotolerance of seedlings. The RTM2 gene product was also shown to contain a large C-terminal region with multiple repeating sequences. INTRODUCTIONSystemic infection of plants by viruses generally requires genome replication in individual cells, cell-to-cell movement through plasmodesmata, and long-distance movement through the phloem Baker et al., 1997). A block at any of these steps, either by active defense responses or by incompatible combinations of viral and host factors, can lead to resistance. Several types of active resistance responses to viruses are known (Baker et al., 1997;Carrington and Whitham, 1998). Dominant or semidominant resistance ( R ) genes that trigger hypersensitive cell death or extreme resistance at initial infection sites, occurring most often in a strain-specific or "gene-for-gene" manner, are well known. The tobacco N gene and the potato Rx gene, which condition resistance against tobacco mosaic virus and potato virus X strains, respectively, belong to the nucleotide binding site, leucine-rich repeat (NBS-LRR) class of R genes (Whitham et al., 1994;Bendahmane et al., 1999). Whereas N confers a hypersensitive response concomitant with localized cell-to-cell movement in inoculated leaves, Rx confers an extreme resistance in initially infected cells (Bendahmane et al., 1995).A second type of active resistance involves gene-silencing-like mechanisms. Virus-induced silencing is adaptive, meaning that a naive host can recognize viral nucleic acids and customize a sequence-specific response (Baulcombe, 1999;Grant, 1999). Thus, virus-induced silencing can limit virus accumulation, promote recovery (in some cases) from a systemic infection, and confer resistance to secondary infections with the same or homologous viruses (Ratcliff et al., 1997;Al-Kaff et al., 1998;Ratcliff et al., 1999). In contrast to resistance triggered by NBS-LRR-type R genes, resistance through silencing appears not to depend on a gene-for-gene recognition event. Passive resistance has been sugge...

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Effective vectors for transformation, expression of heterologous genes, and assaying transposon excision in transgenic plants

Cited by 287 publications

References 41 publications

Cloning of the Arabidopsis RTM1 gene, which controls restriction of long-distance movement of tobacco etch virus

Cloning of the Arabidopsis RTM1 gene, which controls restriction of long-distance movement of tobacco etch virus

The Arabidopsis ABSCISIC ACID-INSENSITIVE2 (ABI2) and ABI1 genes encode homologous protein phosphatases 2C involved in abscisic acid signal transduction.

Arabidopsis RTM2 Gene Is Necessary for Specific Restriction of Tobacco Etch Virus and Encodes an Unusual Small Heat Shock–like Protein

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