Effective separation of prolyl endopeptidase from Aspergillus Niger by aqueous two phase system and its characterization and application

Jiang, Bin; Wang, Meichan; Wang, Xiaojing; Wu, Shuang; Li, Dongmei; Liu, Chunhong; Feng, Zhibiao; Li, Jie

doi:10.1016/j.ijbiomac.2020.12.120

Cited by 30 publications

(17 citation statements)

References 48 publications

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“…The DPPH radical scavenging activity of emulsions was determined according to the method of Jiang et al [32]. Specifically, 0.1 mL of emulsion and 4.0 mL of DPPH solution (0.1 mM) that had been prepared in 95% ethanol were mixed thoroughly, and the solution was kept in the dark at 25 • C for 30 min.…”

Section: Dpph Radical Scavenging Activity Assaymentioning

confidence: 99%

Development of Antioxidant and Stable Conjugated Linoleic Acid Pickering Emulsion with Protein Nanofibers by Microwave-Assisted Self-Assembly

Jiao

Liu

et al. 2021

Foods

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Whey protein isolate nanofibrils (WPNFs) can be used as a novel stabilizer in the Pickering emulsion system to improve the water solubility, stability and bioavailability of lipophilic bioactive ingredients. In this study, conjugated linoleic acid (CLA) and WPNFs were used to prepare a stable Pickering emulsion. We used a transmission electron microscope, low-temperature scanning electron micrographs and other methods to evaluate the micromorphology, surface hydrophobicity and structural units of the obtained WPNFs. Compared with whey protein isolate/CLA Pickering emulsion, the WPNFs/CLA Pickering emulsion has greater ability to remove 2,2-Diphenyl-1-picrylhydrazyl and 2,2′-amino-di(2-ethyl-benzothiazoline sulphonic acid-6) ammonium salt free radicals. Furthermore, the WPNFs/CLA Pickering emulsion has a more stable effect in terms of droplet size and zeta potential over a wider range of ionic strength and temperature conditions. These findings indicate that Pickering emulsion stabilized by WPNFs is more suitable as a carrier of CLA, as it increases the solubility of CLA and has better active applications in biology and food.

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Section: Dpph Radical Scavenging Activity Assaymentioning

confidence: 99%

Development of Antioxidant and Stable Conjugated Linoleic Acid Pickering Emulsion with Protein Nanofibers by Microwave-Assisted Self-Assembly

Jiao

Liu

et al. 2021

Foods

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“…Studies have shown that AN-PEP is capable of degrading gluten in all starches with a gluten content of up to 20mg/kg. The AN-PEP is obtained by fermentation of Aspergillus niger (Jiang et al, 2021;Walter, 2015).…”

Section: Discussionmentioning

confidence: 99%

Recombinant production and characterization of Aspergillus niger prolyl endopeptidase enzyme for gluten-free food production

Şenol

Kaplan

İmamoğlu

et al. 2021

International Journal of Agriculture Environment and Food Sciences

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Gluten is a protein group found in wheat, barley, rye, and oats, known as cereals. When this vegetable protein is introduced into the body, celiac disease can occurs. The use of bacterial and fungal oligopeptidase to ensure the cleavage of gluten into non-toxic fragments are considered a promising alternative for celiac disease. In this study, the Aspergillus niger Prolyl EndoPeptidase (AN-PEP) enzyme was cloned into pET22b vector and recombinantly produced in BL21 (DE3) pLysE cells. PEP enzyme expressed as inclusion body and was recovered by refolding. And N-terminal His-tagged recombinant protein was purified by nickel affinity chromatography. 280 mg AN-PEP enzyme from 1L bacterial culture was purified at very high yield, and this protein was 90% purity. As a result; It has been determined that the recombinantly produced PEP enzyme can digest gluten. This study shows that recombinantly produced AN-PEP (rAN-PEP) has great potential to use in the production processes of gluten-free foods.

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“…At present, there are many methods used to separate bioactive substances, such as protein, lutein and lipids, including ultrafiltration or other membrane separation technologies, 5,6 aqueous two-phase extraction, [7][8][9][10] liquid biphasic flotation 11,12 , amongst others. However, the separation of ⊍-La from whey protein isolate (WPI) increases the production process; therefore, separating ⊍-La directly from cow milk whey can simplify the production process and reduce production costs.…”

Section: Introductionmentioning

confidence: 99%

Direct separation and purification of α‐lactalbumin from cow milk whey by aqueous two‐phase flotation of thermo‐sensitive polymer/phosphate

Jiang

Wang

et al. 2021

J Sci Food Agric

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BACKGROUND: ⊍-lactalbumin (⊍-La) is of great interest to the industry as a result of its excellent functional properties and nutritional value. Aqueous two-phase flotation (ATPF) of thermo-sensitive polymer poly (ethylene glycol-ran-propylene glycol) monobutyl ether (UCON) and KH 2 PO 4 was applied to directly separate and purify ⊍-La from milk whey, which was purposed to simplify the production process and reduced cost of production.RESULTS: The effect of ATPF composition and operating parameters on the flotation efficiency (E) and purity of ⊍-La were investigated. The optimal conditions included 2 min of premixing time, 30 mL min −1 flow velocity and 20 min of flotation time, whereas the composition conditions comprised 35.0 mL 0.18 g mL −1 phosphate solution (containing 10% (cow milk whey/salt solution, v/v) cow milk whey, 50 ppm defoamer and 2 g NaCl) and 5.0 mL of 40% (w/w) UCON solution. Under the optimal conditions, E of ⊍-La was 95.67 ± 1.04% and purity of ⊍-La was 98.78 ± 1.19%. UCON was recovered by a thermally-induced phase separation and reused in next ATPF process without reducing E of ⊍-La. Purified ⊍-La was characterized by several key technologies. The results indicated that ⊍-La in cow milk whey could be directly separated and purified by the ATPF and the purity was satisfactory. Moreover, it was suggested there was no obvious structure difference between the ⊍-La separated by ATPF and the ⊍-La standard.CONCLUSION: The present study enabled the recycling of UCON, providing an effective, economically viable and environmentally friendly approach for the separation and purification of protein.

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Effective separation of prolyl endopeptidase from Aspergillus Niger by aqueous two phase system and its characterization and application

Cited by 30 publications

References 48 publications

Development of Antioxidant and Stable Conjugated Linoleic Acid Pickering Emulsion with Protein Nanofibers by Microwave-Assisted Self-Assembly

Development of Antioxidant and Stable Conjugated Linoleic Acid Pickering Emulsion with Protein Nanofibers by Microwave-Assisted Self-Assembly

Recombinant production and characterization of Aspergillus niger prolyl endopeptidase enzyme for gluten-free food production

Direct separation and purification of α‐lactalbumin from cow milk whey by aqueous two‐phase flotation of thermo‐sensitive polymer/phosphate

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