Effective recovery of poly‐β‐hydroxybutyrate (PHB) biopolymer from <scp><i>C</i></scp><i>upriavidus necator</i> using a novel and environmentally friendly solvent system

Tao, Fei; Cazeneuve, Stacy; Wen, Zhiyou; Wu, Lei; Wang, Tong

doi:10.1002/btpr.2247

Cited by 60 publications

(32 citation statements)

References 27 publications

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“…In the present study, the T m of polymer isolated using the alcoholic method was 179 °C and that obtained with the conventional method reached a T m of 176 °C. These values of T m are comparable with those reported by Fei et al and Fiorese et al for P(3HB) produced by Cupriavidus necator and recovered by extraction with propylene carbonate, acetone and ethanol at different temperatures (Table ).…”

Section: Resultssupporting

confidence: 91%

“…Several methods have been reported for the extraction of P(3HB) from the biomass of different organisms, and although these procedures are known to offer promising results, there are still obstacles for the efficient recovery of the biopolymer. Several studies in the literature have reported the use of nonhalogenated solvents . Most of these reports used polar or semi‐polar compounds, to solubilize nonpolar hydrophobic compounds, such as PHAs; therefore, in most of these studies it was necessary to use high temperatures (120–150 °C), as well as high pressures to improve the plastic extraction.…”

Section: Resultsmentioning

confidence: 99%

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Production and recovery of poly‐3‐hydroxybutyrate [P(3HB)] of ultra‐high molecular weight using fed‐batch cultures of Azotobacter vinelandii OPNA strain

García

Pérez

Castro

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND Poly‐3‐hydroxybutyrate P(3HB) is a bioplastic that is characterized for its biodegradability and biocompatibility and can be used in the biomedical field. The aim of this study was to evaluate the scaling‐up process at pilot level, for the production and recovery of P(3HB) synthesized by Azotobacter vinelandii OPNA strain, based on the use of fed‐batch fermentation coupled to a recovery method using nonhalogenated solvents (ethanol and acetone). RESULTS In fed‐batch cultivations at the 3‐L scale, using yeast extract and sucrose of industrial grade with a carbon:nitrogen ratio of 14 and manipulating the agitation rate from 500 to 700 rpm to give a maximal biomass concentration of 33.8 ± 1.5 g L−1, P(3HB) concentrations of 29 ± 4.5 g L−1 were reached. By simulating the gassed volumetric power input observed in the 3‐L bioreactors at the different agitation rates (500 and 700 rpm), a scaling‐up to 30‐L bioreactors was performed. At this scale, the P(3HB) concentration, and productivity were similar or even better than those values obtained at the smaller scale. The production process was coupled to a P(3HB) separation process, in which the polymer was precipitated with ethanol and washed with acetone, reaching a purity close to 95% and a yield of 85%. The P(3HB) obtained was of ultra‐high molecular mass, with values of 5693 ± 615 kDa. CONCLUSION In this study a successful scaling‐up process for the production of P(3HB) of ultra‐high molecular weight at pilot scale was developed. © 2019 Society of Chemical Industry

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Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Production and recovery of poly‐3‐hydroxybutyrate [P(3HB)] of ultra‐high molecular weight using fed‐batch cultures of Azotobacter vinelandii OPNA strain

García

Pérez

Castro

et al. 2019

J of Chemical Tech & Biotech

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“…Reflux and Soxhlet extractions, with and without biomass pretreatments, have been described in literature for many different solvents [21][22][23][24]. Non-halogenated solvents have been the focus of many researches for their reduced toxicity, although the chlorinated ones, such as chloroform and dichloromethane, are still considered reference solvents because of their high efficiency [22,25,26]. The non-halogenated solvent dimethyl carbonate performs much better than a range of solvents such as diethyl carbonate, propylene carbonate and ethyl acetate and it achieved satisfactory yields of PHA recovery when compared to dichloromethane [27,28].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Green Extraction and Purification of PHA Produced by Mixed Microbial Cultures from Sludge

Reis

Michels

Fajardo

et al. 2020

Water

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Sludge from municipal wastewater treatment systems can be used as a source of mixed microbial cultures for the production of polyhydroxyalkanoates (PHA). Stored intracellularly, the PHA is accumulated by some species of bacteria as energy stockpile and can be extracted from the cells by reflux extraction. Dimethyl carbonate was tested as a solvent for the PHA extraction at different extraction times and biomass to solvent ratios, and 1-butanol was tested for purifying the obtained PHA at different purification times and PHA to solvent ratios. Overall, only a very small difference was observed in the different extraction scenarios. An average extraction amount of 30.7 ± 1.6 g of PHA per 100 g of biomass was achieved. After purification with 1-butanol, a visual difference was observed in the PHA between the tested scenarios, although the actual purity of the resulting samples did not present a significant difference. The overall purity increased from 91.2 ± 0.1% to 98.0 ± 0.1%.

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“…Fei et al, were also reported a novel solvent system for extracting PHB. They recovered PHB from C. necator dry biomass using acetone, ethanol and propylene carbonate (1:1:1).…”

Section: Resultsmentioning

confidence: 99%

An efficient method for the application of PHA‐poor solvents to extract polyhydroxybutyrate from Cupriavidus necator

Aramvash

Gholami-Banadkuki

Seyedkarimi

2016

Biotechnology Progress

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There are many published studies presenting ethanol and acetone as PHAs-poor solvents, where these two solvents are shown to dissolve <2% (w/v) of PHAs at low temperatures. In this study, the suitability of ethanol and acetone for the recovery of PHB at different temperatures (from room temperature to near boiling point) in Cupriavidus necator was investigated. Experiments were performed using response surface methodology to examine the effects of different temperatures and heating incubation times on recovery percentage using the two solvents. The highest recovery percentage (92.3%) and product purity (up to 99%) were obtained with ethanol-assisted extraction at 76°C for 32 min of incubation time. Under these conditions the extracted PHB exhibited a molecular mass of 1.2 × 10 . The present strategy showed that at temperatures near its boiling point, ethanol, as a nonhalogenated solvent, represents a good alternative to halogenated solvents, like chloroform, when PHB recovery is concerned. DSC analysis showed good thermal properties for ethanol- and acetone-extracted biopolymers. GC and H NMR analysis confirmed the extracted biopolymer to be polyhydroxybutyrate of good purity. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1480-1486, 2016.

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Effective recovery of poly‐β‐hydroxybutyrate (PHB) biopolymer from Cupriavidus necator using a novel and environmentally friendly solvent system

Cited by 60 publications

References 27 publications

Production and recovery of poly‐3‐hydroxybutyrate [P(3HB)] of ultra‐high molecular weight using fed‐batch cultures of Azotobacter vinelandii OPNA strain

Production and recovery of poly‐3‐hydroxybutyrate [P(3HB)] of ultra‐high molecular weight using fed‐batch cultures of Azotobacter vinelandii OPNA strain

Optimization of Green Extraction and Purification of PHA Produced by Mixed Microbial Cultures from Sludge

An efficient method for the application of PHA‐poor solvents to extract polyhydroxybutyrate from Cupriavidus necator

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