Effective plasmid pX3 transduction in Lactobacillus delbrueckii by bacteriophage LL-H

Равин, В. К.; Sasaki, Takashi; Räisänen, Liisa; Riipinen, K.-A.; Alatossava, Tapani

doi:10.1016/j.plasmid.2005.12.003

Cited by 16 publications

(9 citation statements)

References 35 publications

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“…It is more likely that there is a requirement for a specific region of pLP712 essential for bacteriophage packaging. This has been reported for high-frequency pX3 plasmid transduction by Lactobacillus delbrueckii bacteriophage LL-H, where there is no evident homology or recombination between the phage and the plasmid (Ravin et al, 2006).…”

Section: Analysis Of Plp712 Deletion Plasmid Derivativesmentioning

confidence: 65%

Molecular characterization and structural instability of the industrially important composite metabolic plasmid pLP712

et al. 2012

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The widely used plasmid-free Lactococcus lactis strain MG1363 was derived from the industrial dairy starter strain NCDO712. This strain carries a 55.39 kb plasmid encoding genes for lactose catabolism and a serine proteinase involved in casein degradation. We report the DNA sequencing and annotation of pLP712, which revealed additional metabolic genes, including peptidase F, D-lactate dehydrogenase and a-keto acid dehydrogenase (E3 complex). Comparison of pLP712 with other large lactococcal lactose and/or proteinase plasmids from L. lactis subsp. cremoris SK11 (pSK11L, pSK11P) and the plant strain L. lactis NCDO1867 (pGdh442) revealed their close relationship. The plasmid appears to have evolved through a series of genetic events as a composite of pGdh442, pSK11L and pSK11P. We describe in detail a scenario by which the metabolic genes relevant to the growth of its host in a milk environment have been unified on one replicon, reflecting the evolution of L. lactis as it changed its biological niche from plants to dairy environments. The extensive structural instability of pLP712 allows easy isolation of derivative plasmids lacking genes for casein degradation and/or lactose catabolism. Plasmid pLP712 is transferable by transduction and conjugation, and both of these processes result in significant molecular rearrangements. We report the detailed molecular analysis of insertion sequence element-mediated genetic rearrangements within pLP712 and several different mechanisms, including homologous recombination and adjacent deletion. Analysis of the integration of the lactose operon into the chromosome highlights the fluidity of the MG1363 integration hotspot and the potential for frequent movement of genes between plasmids and chromosomes in Lactococcus.

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Section: Analysis Of Plp712 Deletion Plasmid Derivativesmentioning

confidence: 65%

Molecular characterization and structural instability of the industrially important composite metabolic plasmid pLP712

et al. 2012

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“…Few transduction systems have been discussed in Lactobacillus species (11)(12)(13). The first high-frequency plasmid transduction system in lactobacilli was developed utilizing L. gasseri bacteriophage adh (14).…”

mentioning

confidence: 99%

Spontaneously Induced Prophages in Lactobacillus gasseri Contribute to Horizontal Gene Transfer

Baugher

Durmaz

Klaenhammer

2014

Appl Environ Microbiol

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Lactobacillus gasseri is an endogenous species of the human gastrointestinal tract and vagina. With recent advances in microbial taxonomy, phylogenetics, and genomics, L. gasseri is recognized as an important commensal and is increasingly being used in probiotic formulations. L. gasseri strain ADH is lysogenic and harbors two inducible prophages. In this study, prophage adh was found to spontaneously induce in broth cultures to populations of ϳ10 7 PFU/ml by stationary phase. The adh prophagecured ADH derivative NCK102 was found to harbor a new, second inducible phage, vB_Lga_jlb1 (jlb1). Phage jlb1 was sequenced and found to be highly similar to the closely related phage LgaI, which resides as two tandem prophages in the neotype strain L. gasseri ATCC 33323. The common occurrence of multiple prophages in L. gasseri genomes, their propensity for spontaneous induction, and the high degree of homology among phages within multiple species of Lactobacillus suggest that temperate bacteriophages likely contribute to horizontal gene transfer (HGT) in commensal lactobacilli. In this study, the host ranges of phages adh and jlb1 were determined against 16 L. gasseri strains. The transduction range and the rate of spontaneous transduction were investigated in coculture experiments to ascertain the degree to which prophages can promote HGT among a variety of commensal and probiotic lactobacilli. Both adh and jlb1 particles were confirmed to mediate plasmid transfer. As many as ϳ10 3 spontaneous transductants/ml were obtained. HGT by transducing phages of commensal lactobacilli may have a significant impact on the evolution of bacteria within the human microbiota. L actic acid bacteria (LAB) are used extensively in foods because they are generally recognized as safe (GRAS) organisms that produce natural preservatives, such as lactic acid and other fermentation by-products (1). Species of LAB are commensal bacteria of the human gastrointestinal, oral, and vaginal tracts and are also utilized as starter cultures in dairy, vegetable, wine, and meat fermentations and as probiotics (2). Probiotics are "live microorganisms, which when administered in adequate amounts confer a health benefit on the host" (3). Potential mechanisms of action include competitive exclusion, such as that shown against diarrheagenic Escherichia coli (4), maintenance of intestinal epithelial barrier function (5), metabolic and antimicrobial effects, enhancement of a balanced microbiota, functional modulation of signal transduction (6), and immunomodulation of innate/adaptive host immunity (7).Genomic sequences of several lactic acid bacteria have revealed multiple temperate phages and phage remnants residing within the genomes (8). The sequence of Lactobacillus gasseri ATCC 33323 (9), an autochthonous bacteria of the gastrointestinal tract (GIT) and a dominant Lactobacillus species of the mouth, GIT, and vaginal microbiota, revealed two adjacent tandem copies of prophage LgaI as well as other nonfunctional phage remnants (10). Potentially, phage transduc...

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“…High-frequency plasmid transduction observed in L. lactis was explained by the shortening of the original plasmid to a size that exactly fitted the phage head [34]. Infection of a new host by bacteriophages has led to successful transfer of bacterial DNA between strains of poorly genetically accessible organisms such as Lactobacillus delbrueckii [35] or even between different LAB species [36]. However, host-specificity is dictated by the combination of phage-encoded receptor binding proteins (RBPs) that associate with the phage base plate and the cell wall polysaccharide and/or proteinaceous receptors on the host surface [37 ].…”

Section: Bacteriophage Transductionmentioning

confidence: 99%

Renaissance of traditional DNA transfer strategies for improvement of industrial lactic acid bacteria

Bron

Marcelli²,

Mulder³

et al. 2019

Current Opinion in Biotechnology

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Effective plasmid pX3 transduction in Lactobacillus delbrueckii by bacteriophage LL-H

Cited by 16 publications

References 35 publications

Molecular characterization and structural instability of the industrially important composite metabolic plasmid pLP712

Molecular characterization and structural instability of the industrially important composite metabolic plasmid pLP712

Spontaneously Induced Prophages in Lactobacillus gasseri Contribute to Horizontal Gene Transfer

Renaissance of traditional DNA transfer strategies for improvement of industrial lactic acid bacteria

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