Effective on-line extraction of drugs from plasma using a restricted-access media column in column-switching HPLC equipped with a dilution system: Application to the simultaneous determination of ER-118585 and its metabolites in canine plasma

Yamamoto, Eiichi; Kato, Takashi; Mano, Nariyasu; Asakawa, Naoki

doi:10.1016/j.jpba.2009.02.022

Cited by 14 publications

(6 citation statements)

References 32 publications

(37 reference statements)

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“…However, small molecules can penetrate through to the inner surface and are retained in the stationary phases. The direct analysis of drugs and metabolites in biological fluids, such as plasma, using RAM with HPLC can rapidly yield sensitive chemical identifications [32,33].…”

Section: Selection Of the Spe Columnmentioning

confidence: 99%

“…Therefore, we attempted to develop simultaneous measurement of drugs and liposome-encapsulated drugs by taking into account the differences in physicochemical properties and sizes between the drugs and the liposomes. This report describes an effective and simple on-line SPE of released and liposomal drugs in the plasma using a column-switching HPLC system for DXR-containing liposome formulations as the model drug and a methylcellulose-immobilized octadecylsilylated silica (MC-ODS) SPE column [28], which is a restricted access media (RAM) column [29][30][31] capable of direct plasma injection [28,32].…”

Section: Introductionmentioning

confidence: 99%

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Direct, simultaneous measurement of liposome-encapsulated and released drugs in plasma by on-line SPE–SPE–HPLC

Yamamoto

Hyôdô

Ohnishi

et al. 2011

Journal of Chromatography B

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Section: Selection Of the Spe Columnmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Direct, simultaneous measurement of liposome-encapsulated and released drugs in plasma by on-line SPE–SPE–HPLC

Yamamoto

Hyôdô

Ohnishi

et al. 2011

Journal of Chromatography B

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“…The pairing of these two characteristics allows SEC selection using the semipermeable methoxycellulose coating along with the pores of the porous silica gel particles to effectively exclude unwanted matrix interferences . This MC‐ODS trap has been heavily used in method development and is the focus of a number of application studies .…”

Section: Sps Phase Trap Columnsmentioning

confidence: 99%

“…Yamamoto et al have recently demonstrated the use of this MC‐ODS RAM column toward the metabolic profiling of a drug compound from canine plasma . The work features injection of canine plasma samples directly onto the trap column.…”

Section: Sps Phase Trap Columnsmentioning

confidence: 99%

Restricted access media as a streamlined approach toward on‐line sample preparation: Recent advancements and applications

Yang

Fan

Classon³

et al. 2013

J of Separation Science

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Restricted access media (RAM) as an alternative to traditional sample preparation strategies are reviewed. RAM comprise chromatographic packing materials that combine, typically, a restrictive outer surface to exclude the retention of large biomolecules, which are common interferences in biological fluids, with retentive inner pores or phases to capture analytes of interest. Through the years, a variety of RAM formats have been created, including internal surface phases, semipermeable phases, and molecularly imprinted polymer phases. Many phases are commercially available through a variety of manufacturers. The use of on-line sample preparation using RAM can increase throughput, recovery, and ease of use for sample preparation of complex biological matrices. The state-of-the-art with respect to production and use of these media for a variety of applications is covered.

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“…and a methylcellulose-immobilized octadecylsilylated silica SPE column, 24) which is a restricted access media column [25][26][27] capable of direct plasma injection. 24,28) This direct plasma injection approach using the proposed SPE-SPE-HPLc system made it possible to simultaneously measure the encapsulated and released drugs in plasma. 23) The methodology enabled us to determine the in vivo properties of liposomes and contribute to the efficient creation of liposomal drug product.…”

Section: Fig 2 Abc Phenomenon Of Peg-modified Liposomesmentioning

confidence: 99%

Development of Liposomal Anticancer Drugs

Hyôdô

Yamamoto

Suzuki

et al. 2013

Biological & Pharmaceutical Bulletin

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Liposomes are drug delivery systems that can alter the pharmacokinetic properties of compounds. The adverse effects of anticancer agents are a limiting factor for cancer chemotherapy, therefore, liposomal formulations have the potential to improve the therapeutic efficacy of anticancer agents by enhancing their accumulation in tumors and reducing non-selective distribution to normal tissues, which is known as the enhanced permeability and retention effect. To develop a liposomal anticancer agent as a drug product, its formulation must be designed to ensure its quality until it is administered to patients and to exert maximum potency in clinical use rather than in animal experiments. The chemical stability and physicochemical stability of the ingredients are key factors in the design of liposomal formulations. Drug release rates are critical factors in the therapeutic efficacy of liposomal drug products because the encapsulated drug has no pharmacological activity, and only released drug can exert antitumor/toxic activities. Liposomes should maintain the drug in a stable state in the circulation and then promptly release it after accumulation in the target tissue in order to achieve a sufficient drug concentration. To understand the profile of the formulation and to guarantee the quality of drug product, a reliable analytical method that can determine the released and encapsulated drugs in biological fluids is required. Simple online solid phase extractions of the released and encapsulated drugs using a column-switching HPLC system meet the requirements and this system enables accurate in vitro release testing and in vivo pharmacokinetic evaluation. This review introduces the process of liposomal drug product development from various viewpoints.

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Effective on-line extraction of drugs from plasma using a restricted-access media column in column-switching HPLC equipped with a dilution system: Application to the simultaneous determination of ER-118585 and its metabolites in canine plasma

Cited by 14 publications

References 32 publications

Direct, simultaneous measurement of liposome-encapsulated and released drugs in plasma by on-line SPE–SPE–HPLC

Direct, simultaneous measurement of liposome-encapsulated and released drugs in plasma by on-line SPE–SPE–HPLC

Restricted access media as a streamlined approach toward on‐line sample preparation: Recent advancements and applications

Development of Liposomal Anticancer Drugs

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