Effective micropropagation of kale (Brassica oleracea convar. Acephala var. sabellica): one of the most important representatives of cruciferous crops

Kamińska, M.; Śliwińska, Elwira

doi:10.1007/s11240-023-02497-4

Cited by 3 publications

(1 citation statement)

References 48 publications

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“…It was explained before that the use of MS media for shoot propagation and root induction have been widely performed in Brassica species (Gerszberg et al, 2015;Pavlovic et al, 2010;Rahman et al, 2021;Farooq et al, 2023;Kaminska & Sliwinska, 2023). The use of growth regulator is common in the tissue culture of Brassica species (for review, see Ravandar et al, 2017;Gerszberg, 2018).…”

Section: Micropropagation Of Cabbage Varieties For Conservation and P...mentioning

confidence: 99%

In vitro Propagation of Singgalang Cabbage (Brassica oleracea var. capitata L.) on Murashige and Skoog Modification Media for Preservation Purpose

Sari,

Idris

2024

JBT

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Singgalang Cabbage is one of the local cabbage cultivated on the foothills of Mount Singgalang, West Sumatra. Recently, the existence of this cabbage has decreased, so conservation and preservation efforts need to be carried out. This research was conducted to develop method for in vitro propagation and preservation of singgalang cabbage. The Murashige and Skoog (MS) were used as basal media with two experimental stages, i.e., shoot initiation with 6-Benzylaminopurine (BAP), and root induction and plantlet preservation with modification of MS media. The nodal and shoots were used as explants. The results showed that increment of BAP concentrations gave a significant effect on shoot initiation after 60 days of treatment. MS media-enriched with BAP 2 mg/L gave significant increment of shoots (4 shoots/nodus) and leaves (11.67) numbers when compared to other treatments. For root induction and plantlet preservation, it was found that the earlier of root formation was observed in modification of MS media at ½ and ¼ strength. Meanwhile, MS media at ⅛ strength was observed to be better media for plant height increment (4.75 cm) when compared to other treatments. It was found that the plantlets survived and grew well after 120 days under in vitro condition.

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Section: Micropropagation Of Cabbage Varieties For Conservation and P...mentioning

confidence: 99%

In vitro Propagation of Singgalang Cabbage (Brassica oleracea var. capitata L.) on Murashige and Skoog Modification Media for Preservation Purpose

Sari,

Idris

2024

JBT

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Optimizing callus formation in the endangered medicinal tree Hydnocarpus macrocarpa using phytohormone

Marimuthu,

Muthuchelian

2024

South African Journal of Botany

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Comprehensive elucidation of the differential physiological kale response to cytokinins under in vitro conditions

Kamińska,

Styczynska,

Szakiel

et al. 2024

BMC Plant Biol

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Background Kale, a versatile cruciferous crop, valued for its pro-health benefits, stress resistance, and potential applications in forage and cosmetics, holds promise for further enhancement of its bioactive compounds through in vitro cultivation methods. Micropropagation techniques use cytokinins (CKs) which are characterized by various proliferative efficiency. Despite the extensive knowledge regarding CKs, there remains a gap in understanding their role in the physiological mechanisms. That is why, here we investigated the effects of three CKs – kinetin (Kin), 6-benzylaminopurine (BAP), and 2-isopentenyladenine (2iP) – on kale physiology, antioxidant status, steroidal metabolism, and membrane integrity under in vitro cultivation. Results Our study revealed that while BAP and 2iP stimulated shoot proliferation, they concurrently diminished pigment levels and photosynthetic efficiency. Heightened metabolic activity in response to all CKs was reflected by increased respiratory rate. Despite the differential burst of ROS, the antioxidant properties of kale were associated with the upregulation of guaiacol peroxidase and the scavenging properties of ascorbate rather than glutathione. Notably, CKs fostered the synthesis of sterols, particularly sitosterol, pivotal for cell proliferation and structure of membranes which are strongly disrupted under the action of BAP and 2iP possibly via pathway related to phospholipase D and lipoxygenase which were upregulated. Intriguingly, both CKs treatment spurred the accumulation of sitostenone, known for its ROS scavenging and therapeutic potential. The differential effects of CKs on brassicasterol levels and brassinosteroid (BRs) receptor suggest potential interactions between CKs and BRs. Conclusion Based on the presented results we conclude that the effect evoked by BAP and 2iP in vitro can improve the industrial significance of kale because this treatment makes possible to control proliferation and/or biosynthesis routes of valuable beneficial compounds. Our work offers significant insights into the nuanced effects of CKs on kale physiology and metabolism, illuminating potential avenues for their application in plant biotechnology and medicinal research.

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Effective micropropagation of kale (Brassica oleracea convar. Acephala var. sabellica): one of the most important representatives of cruciferous crops

Cited by 3 publications

References 48 publications

In vitro Propagation of Singgalang Cabbage (Brassica oleracea var. capitata L.) on Murashige and Skoog Modification Media for Preservation Purpose

In vitro Propagation of Singgalang Cabbage (Brassica oleracea var. capitata L.) on Murashige and Skoog Modification Media for Preservation Purpose

Optimizing callus formation in the endangered medicinal tree Hydnocarpus macrocarpa using phytohormone

Comprehensive elucidation of the differential physiological kale response to cytokinins under in vitro conditions

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