Effective microbiological decontamination of dental healing abutments colonised with Rothia aeria by a diode laser as a helpful step towards successful implantoprosthetic therapy

Abstract

The aim of the study was to find variant of diode laser (λ = 810 nm) irradiation, which ensures elimination of unwanted microorganisms, including Rothia aeria, from dental healing abutments, and consequently accelerates process of wound healing in implantologically treated patients. The scope of the study included identification of the most contaminated areas on healing abutments, identification of microorganisms inhabiting various environments of oral cavities, assessment of effectiveness of various laser decontamination parameters against detected microorganisms (preliminary studies) and assessment of wound healing in patients after applying abutments with low roughness and optimal variant of laser irradiation (clinical studies). Imaging of surfaces of the healing abutments was performed using vertical scanning interferometry, scanning electron microscopy and optical microscopy. Microorganisms inhabiting the healing abutments, teeth and saliva from tested patients were identified using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. Three programmes of near-infrared diode laser at average powers of 1.00-3.84 W with two variants of exposure time were used for optimisation of laser parameters. Observation of wound healing was performed for 100 patients during 20 days after installation of abutments. On surfaces of the used healing abutments, a large number of microorganisms, with a predominance of R. aeria, were found. Irradiation with periimplantitis surgical programme for 30 s resulted in 99-100% reduction in the number of R. aeria and other microorganisms, depending on type of abutment (in vivo). The use of diode laser in the selected variant accelerates wound healing and provides complete elimination of pathogenic R. aeria and other microorganisms inhabiting surfaces of the healing abutments without marks.

The Use of the Diode Laser against the Microbiome on Composites Closing the Screw Access Hall (Sah) in the Reconstruction of Dental Implants: Ex Vivo Studies

“…One of the methods that can reduce the number of microorganisms on the composites’ surface is the use of a diode laser, the irradiation of which can supplement the implantological treatment procedure. The effectiveness of the diode laser against microorganisms inhabiting porcelain, zircon, and titanium was confirmed by Wawrzyk et al [ 20 , 21 , 22 ]. The method of decontamination with the diode laser used by Wawrzyk et.al.…”

Optimization of the Process of Eliminating Microorganisms Harmful to Human Health and Threatening Objects Isolated from Historical Materials from the Auschwitz-Birkenau State Museum in Poland (A-BSM) Collection with the Use of Ethanol in the Form of Mist

“…The A-BSM successfully performs disinfection with a diode laser, for which in the medical area, a reduction of bacteria and fungi from 60% to 100% was achieved on various materials [ 18 , 19 , 20 , 21 , 22 ]; on historical collagen material in the A-BSM, a reduction of 78–92% was achieved, and on cellulose, 90–100% was achieved [ 8 , 23 , 24 ].…”

Diode Laser as an Adjunctive Treatment for Peri-implant Mucositis: A Systematic Review and Meta-analysis

“…Moreover, diode laser irradiation on healing abutments has markedly eliminated the predominant pathogenic bacteria and accelerated wound healing without any harmful effects on the evaluated implant material. 53 , 54 …”