Effective loading of incompatible drugs into nanosized vesicles: a strategy to allow concurrent administration of furosemide and midazolam in simulated clinical settings

Fathi, Heba A.; Yousry, Carol; Elsabahy, Mahmoud; El-Badry, Mahmoud; El‐Gazayerly, Omaima N.

doi:10.1016/j.ijpharm.2023.122852

Cited by 4 publications

(2 citation statements)

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“…Interestingly, surfactants with a high hydrophilic–lipophilic balance exhibit similar behavior when added to the vesicle membrane [ 36 ]. It is possible that the effect on vesicle size may correlate with the molecular weight of the encapsulated biocompound, with a more noticeable vesicle size increase when encapsulating high-molecular-weight biocompounds [ 26 ]. Other studies have demonstrated that the presence of certain additives, such as some costabilizers, leads to the formation of much larger niosomes.…”

Section: Discussionmentioning

confidence: 99%

“…For the antimicrobial tests, 25 mL samples containing both empty and protein-loaded niosomes were ultracentrifuged at 35,000 rpm for 50 min at 4 °C (Optica TM MAX ultracentruge, Beckman Coulter). The supernatant containing free non-encapsulated protein was removed, and the pellet was resuspended with 250 µL of PBS or Milli-Q water in order to remove surfactants, empty niosomes, and contaminants from the suspension [ 26 ].…”

Section: Methodsmentioning

confidence: 99%

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Phage Lytic Protein CHAPSH3b Encapsulated in Niosomes and Gelatine Films

Marchianò,

Duarte,

Agún

et al. 2024

Microorganisms

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Antimicrobial resistance (AMR) has emerged as a global health challenge, sparking worldwide interest in exploring the antimicrobial potential of natural compounds as an alternative to conventional antibiotics. In recent years, one area of focus has been the utilization of bacteriophages and their derivative proteins. Specifically, phage lytic proteins, or endolysins, are specialized enzymes that induce bacterial cell lysis and can be efficiently produced and purified following overexpression in bacteria. Nonetheless, a significant limitation of these proteins is their vulnerability to certain environmental conditions, which may impair their effectiveness. Encapsulating endolysins in vesicles could mitigate this issue by providing added protection to the proteins, enabling controlled release, and enhancing their stability, particularly at temperatures around 4 °C. In this work, the chimeric lytic protein CHAPSH3b was encapsulated within non-ionic surfactant-based vesicles (niosomes) created using the thin film hydrating method (TFH). These protein-loaded niosomes were then characterized, revealing sizes in the range of 30–80 nm, zeta potentials between 30 and 50 mV, and an encapsulation efficiency (EE) of 50–60%. Additionally, with the objective of exploring their potential application in the food industry, these endolysin-loaded niosomes were incorporated into gelatine films. This was carried out to evaluate their stability and antimicrobial efficacy against Staphylococcus aureus.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%