Effective Light-Upon-Extension Real-Time PCR Primer Systems for Rapid Detection of Human Viruses

Kalvatchev, Z.; Tsekov, Iliya; Slavov, Svetoslav Nanev; Draganov, P.

doi:10.1309/lmly7bg3d1ojnkho

Cited by 2 publications

(2 citation statements)

References 19 publications

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“…Similarly we expected a low number of sequences in the tested samples and employed assays with high sensitivity such as real‐time PCR, giving a better chance for detection of viral genomes. Another novel real‐time PCR technique that can be of use due to its high sensitivity and specificity is Light Upon Extension (LUX) real‐time PCR [Kalvatchev et al, 2010].…”

Section: Discussionmentioning

confidence: 99%

Prevalence of JC polyomavirus genomic sequences from the large t‐antigen and non‐coding control regions among bulgarian patients with primary brain tumors

Tsekov

Ferdinandov

Bussarsky

et al. 2011

Journal of Medical Virology

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A total of 111 fresh brain biopsies from patients with primary brain tumors were examined for JC polyomavirus sequences from the Large T antigen encoding region (LT) and the viral non-coding control region (NCCR). SYBR Green and TaqMan real-time polymerase chain reaction assays were used. In the glioblastoma group of 39 patients 48.7% were positive for LT sequences. Among the astrocytoma group (19 patients) and the oligodendroglioma group (12 patients) 31.6% and 33.3% were also positive. The prevalence of LT genomic sequences among the other groups was as follows: in 2 out of 3 oligoastrocytomas; in 3 out 5 gangliogliomas; in 2 out of 5 meduloblastomas; in 1 out 3 pineocytomas; and in none of the tested 5 ependimomas. All positive samples had a late threshold cycle that varied from 36 to 49, indicative of very low starting viral number. Only 21 of all the 111 samples were positive for NCCR. Low copy number in range of 10-1,000 was present. Notably, only 8 of all NCCR positive specimens were also LT positive. It might be suggested that the disproportion between the results for LT and NCCR is either due to clonally integrated LT fragments, with loss of genetic material, or changes in the NCCR. The latter would alter the productive course of the infection and may establish a premise for continuous interaction of viral regulatory proteins with cell molecules that are responsible for the control of the cell cycle. This may lead subsequently to malignant transformation.

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Section: Discussionmentioning

confidence: 99%

Prevalence of JC polyomavirus genomic sequences from the large t‐antigen and non‐coding control regions among bulgarian patients with primary brain tumors

Tsekov

Ferdinandov

Bussarsky

et al. 2011

Journal of Medical Virology

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“…Serum JCPyV was investigated in conjunction with assessing virus in the urine specimen. Urine JCPyV had been consecutively positive with viral load of >10 6 copies/mL but with negative evidence of viremia on several occasions . A diagnosis of JCPyVAN was made and the transplant team aimed to further reduce immunosuppressive drugs for better control of the virus.…”

Section: Case Reportmentioning

confidence: 99%

Absence of JC polyomavirus (JCPyV) viremia in early post‐transplant JCPyV nephropathy: A case report

Janphram

Worawichawong

Disthabanchong

et al. 2017

Transplant Infectious Dis

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JC polyomavirus (JCPyV)-associated nephropathy (JCPyVAN) occurs in <3% of PVAN cases after renal transplantation. We report the first confirmed case to our knowledge of JCPyVAN diagnosed by kidney biopsy in the early 6 months post transplant in Thailand. In this case report, recovery of renal allograft function was not observed after reduction of immunosuppressive agents and administration of intravenous immunoglobulin and cidofovir. Despite persistent JCPyV viruria, no significant further decline in allograft function was documented at 15 months post transplant. K E Y W O R D Sallograft dysfunction, JC virus nephropathy, kidney transplant

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Effective Light-Upon-Extension Real-Time PCR Primer Systems for Rapid Detection of Human Viruses

Cited by 2 publications

References 19 publications

Prevalence of JC polyomavirus genomic sequences from the large t‐antigen and non‐coding control regions among bulgarian patients with primary brain tumors

Prevalence of JC polyomavirus genomic sequences from the large t‐antigen and non‐coding control regions among bulgarian patients with primary brain tumors

Absence of JC polyomavirus (JCPyV) viremia in early post‐transplant JCPyV nephropathy: A case report

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