Effective immobilization of tyrosinase via enzyme catalytic polymerization of l -DOPA for highly sensitive phenol and atrazine sensing

Guan, Ying; Liu, Lanjunzi; Chen, Chao; Kang, Xiuzhi; Xie, Qingji

doi:10.1016/j.talanta.2016.07.003

Cited by 39 publications

(18 citation statements)

References 53 publications

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“…An interesting biosensor for detection of atrazine was prepared by using a composite obtained through the polymerization of L-DOPA in presence of tyrosinase and thereafter immobilized on a gold electrode in the presence of Nafion. The amperometric detection of atrazine was based on its inhibition effect on tyrosinase catalytic activity and the biosensor used proved good stability, high sensitivity, and precision [ 84 ].…”

Section: Other Enzymesmentioning

confidence: 99%

Advances in Enzyme-Based Biosensors for Pesticide Detection

et al. 2018

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The intensive use of toxic and remanent pesticides in agriculture has prompted research into novel performant, yet cost-effective and fast analytical tools to control the pesticide residue levels in the environment and food. In this context, biosensors based on enzyme inhibition have been proposed as adequate analytical devices with the added advantage of using the toxicity of pesticides for detection purposes, being more “biologically relevant” than standard chromatographic methods. This review proposes an overview of recent advances in the development of biosensors exploiting the inhibition of cholinesterases, photosynthetic system II, alkaline phosphatase, cytochrome P450A1, peroxidase, tyrosinase, laccase, urease, and aldehyde dehydrogenase. While various strategies have been employed to detect pesticides from different classes (organophosphates, carbamates, dithiocarbamates, triazines, phenylureas, diazines, or phenols), the number of practical applications and the variety of environmental and food samples tested remains limited. Recent advances focus on enhancing the sensitivity and selectivity by using nanomaterials in the sensor assembly and novel mutant enzymes in array-type sensor formats in combination with chemometric methods for data analysis. The progress in the development of solar cells enriched the possibilities for efficient wiring of photosynthetic enzymes on different surfaces, opening new avenues for development of biosensors for photosynthesis-inhibiting herbicides.

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Section: Other Enzymesmentioning

confidence: 99%

Advances in Enzyme-Based Biosensors for Pesticide Detection

et al. 2018

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“…These enzymatically prepared electrodes retained more than double the sensitivity upon immobilization and a more than two-fold increase in stability as compared to the glutaraldehyde-prepared ones [ 91 ]. The polymerization of L-DOPA by tyrosinase has also been used to synthesize a melanin-like polymeric matrix for the entrapment of glucose oxidase and tyrosinase for amperometric biosensing, reaching a 10 nM detection limit for phenol [ 92 , 93 ]. Enzymatic protein immobilization strategies are highly specific and can be applied with proteins that present surface-exposed target residues.…”

Section: Protein Immobilization Approachesmentioning

confidence: 99%

From Protein Features to Sensing Surfaces

Faccio

2018

Sensors

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Proteins play a major role in biosensors in which they provide catalytic activity and specificity in molecular recognition. However, the immobilization process is far from straightforward as it often affects the protein functionality. Extensive interaction of the protein with the surface or significant surface crowding can lead to changes in the mobility and conformation of the protein structure. This review will provide insights as to how an analysis of the physico-chemical features of the protein surface before the immobilization process can help to identify the optimal immobilization approach. Such an analysis can help to preserve the functionality of the protein when on a biosensor surface.

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“…For example, tetraruthenated porphyrin (TRP) consists of a tetrapyridylporphyrin (TPyP) along with four Ru (II) complexes in the periphery of the macrocycle. These kinds of macrocycles are particularly attractive because they display unusual electrocatalytic [21,22] and photoelectrochemical [23,24] properties. In particular, these porphyrins have been used in the electroanalytical detection of S (IV) oxoanions [25][26][27] and the electrocatalytic reduction processes of O2 [28] and CO2 [29,30].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Determination of N,n-Dimethyltryptamine in Water Based on Tetraruthenated Porphyrins and Ionic Liquid Modified Electrodes

Plaza

Piña²,

Herrera

et al. 2020

J. Chil. Chem. Soc.

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N,N-dimethyltryptamine (DMT) is a short-acting psychotropic agent when administered parenterally and is not active orally due to its rapid degradation by monoamine oxidase enzyme type A. There is growing interest in the therapeutic potential of DMT due to recent clinical data that has shown that produces antidepressant effects in humans. It has also been found that psychedelics that possess the central structure of DMT may have value as medications and cannot simply be labeled as drugs with the potential for abuse. The incorporation of ionic liquid and tetraruthenated zinc porphyrin to glassy carbon electrode enhanced the voltammetric determination of DMT compared to the bare glassy carbon electrode. These modified electrodes showed good performance for DMT oxidation. These results translate into a low detection limit (1.75 µM), short response time, satisfactory linear concentration range, very good stability and reproducibility. Thus, his methodology can be a feasible and inexpensive way to detect DMT in water and presents potential to be applied in the detection of DMT in urine.

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Effective immobilization of tyrosinase via enzyme catalytic polymerization of l -DOPA for highly sensitive phenol and atrazine sensing

Cited by 39 publications

References 53 publications

Advances in Enzyme-Based Biosensors for Pesticide Detection

Advances in Enzyme-Based Biosensors for Pesticide Detection

From Protein Features to Sensing Surfaces

Electrochemical Determination of N,n-Dimethyltryptamine in Water Based on Tetraruthenated Porphyrins and Ionic Liquid Modified Electrodes

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