Effective Enzyme-Producing Bacteria Isolated from Diversified Thai Rice and Native Thai Bees (Cavity Nesting Honey Bees) and their Potential for Production of Protease Enzymes

Abstract

Background: Thailand is one of the most bio-diversified countries in Southeast Asia, especially regarding plants, animals and microbes that impact directly on human and animal life. Methods: Of the 201 bacterial isolates that were screened from Thai rice and bees in Ratchaburi and Chiang Mai provinces, Thailand, most were classified in the genus Bacillus. Result: From the morphological test and 16S ribosomal DNA sequence study, three strains, Bacillus flexus KRptI_S2, Microbacterium paraoxydans C13HN2 and Paenibacillus hunanensis KRrb_T2, could produce amylase and protease enzyme and non-hemolytic activity at higher temperature. The highest enzymatic activity of protease was produced by P. hunanensis KRrb_T2 (378.9967±1.335 U.mL -1 ) at 36 h. Conclusion: In summary, the bacteria from Thai rice and bees could be potential suitable sources of protease production for development by the food and beverage industries globally.

Microbiological Diversity and Associated Enzymatic Activities in Honey and Pollen from Stingless Bees from Northern Argentina

“…This type of fermentation is carried out by Bacillus spp. [45], which is consistent with the various hydrolytic activities reported in this work. Finally, the best values of amylolytic activity were reported in two isolates from the same niche, 91_Tf_h_17 and 96_Tf_h_17, both of which were identified as Escherichia spp.…”

Assessment of Lipase Enzyme Production Potential of Bacteria Isolated from Oryza Sativa (Rice) Rhizosphere in Sokoto, Nigeria

“…The rice has a term known as rhizosphere referring to an area on the soil compartments serving as habitat to microorganisms in the ecosystem. It lies within the roots, influenced by the plants and acts as an interface to the plant, soil, and microbial interactions as influenced by the prevailing environment (Umar et al, 2019;Ayoola, 2020, Chumphon et al, 2022. Rhizosphere definitely has a rhizobacteria that helps in improving plant growth through intervention such as nutrients, phosphate solubilization, pathogens resistance, nitrogen fixation etc (Patel andDesai, 2015, Tsegaye et al, 2019).…”

“…Enzymes are organic catalysts made by organism to speed up the rate of chemical reactions (Gopalakrishnan et al, 2013). These enzymes are engaged in a diverse array of processes in the biological system and are championed for several industrial activities (Ayoola, 2020).…”

“…They can also be useful in esterification, transesterification, amino lysis, and lactonization. Therefore, very beneficial at industrial level (Ayoola, 2020).…”

“…Oryza sativa (Rice) is a major staple foodstuff consumed by about half of the inhabitants on earth (Ayoola, 2020, Chumphon et al, 2022. In terms of size of area utilized for production, rice remained the largest crop ever in the world.…”