Effective detergent/lipid ratios in the solubilization of phosphatidylcholine vesicles by Triton X‐100

Partearroyo, M.Aránzazu; Urbaneja, Marı́a A.; Goñi, Félix M.

doi:10.1016/0014-5793(92)80424-f

Cited by 73 publications

(55 citation statements)

References 20 publications

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“…Also in agreement with the above, the membrane/water partition equilibrium constant K (34 mM -1) is much higher than those of, e.g. Triton X-100 (1.9 mM -1 [12]) or Hecameg (0.08 mM -1 [13]). The Re sAT and Re sOL values for palmitoylcarnitine in egg phosphatidylcholine bilayers are respectively of 0.78 and 1.97 [11].…”

Section: Palmitoylcarnitine As a Surfaetantsupporting

confidence: 81%

“…As was mentioned above, these values are similar to the corresponding ones for biochemically used surfactants, e.g. Triton X-100 (0.71 and 3.0 [12]) or Hecameg (1.4 and 2.6 [13]). In summary, palmitoylcarnitine is, from the physico-chemical point of view, a surfactant with solubilising properties equivalent to many natural or synthetic detergents that are commonly in use in biological laboratories.…”

Section: Palmitoylcarnitine As a Surfaetantsupporting

confidence: 79%

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Palmitoylcarnitine, a surface‐active metabolite

1996

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Palmitoylcarnitine is a well-known intermediate in mitochondrial fatty acid oxidation. Less known are its properties as a snrfactant, with a capacity to solubilize biological membranes similar to that of many synthetic detergents used in the biochemical laboratory. Some of the physico-chemical properties of palmitoylcarnitine may help to explain the need for coenzyme A-carnitine-coenzyme A acyl exchange during mitochondrial fatty acid import. The amphiphilic character of palmitoylcarnitine may also explain its proposed involvement in the pathogenesis of myocardial ischemia.

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Section: Palmitoylcarnitine As a Surfaetantsupporting

confidence: 81%

Section: Palmitoylcarnitine As a Surfaetantsupporting

confidence: 79%

Palmitoylcarnitine, a surface‐active metabolite

1996

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“…According to their physicochemical properties -such as charge, critical micelle concentration (CMC), aggregation number and hydrophilic-lipophilic balance (HLB) -different interactions occur and membrane proteins are denatured or not (4,7). As for the lipids, quantitative studies of membrane solubilization show a strong correlation between these physicochemical properties of detergents (2,(8)(9)(10) and their lytic effect.…”

Section: Introductionmentioning

confidence: 99%

Solubilization of human erythrocyte membranes by ASB detergents

Domingues

Malheiros

Paula

2008

Braz J Med Biol Res

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Understanding the membrane solubilization process and finding effective solubilizing agents are crucial challenges in biochemical research. Here we report results on the interaction of the novel linear alkylamido propyl dimethyl amino propanosulfonate detergents, ASB-14 and ASB-16, with human erythrocyte membranes. An estimation of the critical micelle concentration of these zwitterionic detergents (ASB-14 = 100 µM and ASB-16 = 10 µM) was obtained using electron paramagnetic resonance. The amount of proteins and cholesterol solubilized from erythrocytes by these detergents was then determined. The hemolytic activities of the ASB detergents were assayed and the detergent/lipid molar ratios for the onset of hemolysis (R e sat ) and total lysis (R e sol ) were calculated, allowing the determination of the membrane binding constants (K b ). ASB-14 presented lower membrane affinity (K b = 7050 M -1 ) than ASB-16 (K b = 15610 M -1 ). The amount of proteins and cholesterol solubilized by both ASB detergents was higher while R e sat values (0.22 and 0.08 detergent/lipid for ASB-14 and ASB-16, respectively) were smaller than those observed with the classic detergents CHAPS and Triton X-100. These results reveal that, besides their well-known use as membrane protein solubilizers to enhance the resolution of two dimensional electrophoresis/ mass spectrometry, ASB-14 and ASB-16 are strong hemolytic agents. We propose that the physicochemical properties of ASB detergents determine their membrane disruption efficiency and can help to explain the improvement in the solubilization of membrane proteins, as reported in the literature.

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“…Smaller value of D T REL than D T SAT was observed for Triton X-100 in EYPC and soya phosphatidylcholine bilayers 14,46 and cationic surfactant SDS in EYPC bilayers. 43 CHOL protects EYPC liposomes from solubilization and caused a decrease of SDS partition coefficient.…”

Section: Resultsmentioning

confidence: 96%

Cholesterol protects phosphatidylcholine liposomes from N,N-dimethyldodekanamine N-oxide influence

Huláková

Gallová

Devı́nsky

2015

ACSi

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The interaction of N, N-dimethyl-1-dodecanamine N-oxide (C 12 NO) with egg yolk phosphatidylcholine (EYPC) liposomes containing cholesterol (CHOL) was studied. The perturbation of CHOL-EYPC bilayers in unilamellar liposomes (ULL) was observed by the leakage of fluorescence probe calcein. Weak leakage is observed at low surfactant concentration c C12NO (minimal perturbation of the bilayer) followed by an intensive leakage at a middle c C12NO (creation of pores). No change in fluorescence intensity was measured at high c C12NO (calcein totally released from liposomes). The higher CHOL amount in the bilayer, the more surfactant is needed to create pores in the bilayer. Solubilization of CHOL-EYPC ULL induced by C 12 NO was studied turbidimetrically. The solubilization curve consists of three parts: saturation of bilayer at low c C12NO (liposomes are preserved), followed by solubilization (liposome -mixed micelle transition) and post-solubilization. The C 12 NO concentration needed for the onset of the solubilization raise with the increase of n CHOL :n EYPC . The structure of liposomes is still preserved at total calcein release for all n CHOL :n EYPC .

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Effective detergent/lipid ratios in the solubilization of phosphatidylcholine vesicles by Triton X‐100

Cited by 73 publications

References 20 publications

Palmitoylcarnitine, a surface‐active metabolite

Palmitoylcarnitine, a surface‐active metabolite

Solubilization of human erythrocyte membranes by ASB detergents

Cholesterol protects phosphatidylcholine liposomes from N,N-dimethyldodekanamine N-oxide influence

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