Effective control of a suspected cyromazine‐resistant strain of <i><scp>L</scp>ucilia cuprina</i> using commercial spray‐on formulations of cyromazine or dicyclanil

Baker, K.; Pf, Rolfe; George, A J; Vanhoff, KJ; Kluver, PF; Bailey, J Brofft

doi:10.1111/avj.12247

Cited by 7 publications

(4 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Currently, the prophylaxis against flystrike relies on synthetic insecticides, such as organophosphates and insect growth regulators (benzoylphenyl ureas, cyromazine and dicyclanil) [16; 17; 12] and, especially for Merino lambs in Australia's extensive wool industry, on painful surgical husbandry procedures such as the docking and the mulesing [18; 19]. However, the side effects of synthetic insecticides, such as the development of insect resistance [20], the harmful effects on sheep [21], farmers [22], and the environment [23], as well as the rising concerns about animal welfare [24] have made alternative strategies a high priority.…”

Section: Introductionmentioning

confidence: 99%

Toxicity and oviposition deterrence of essential oils of Clinopodium nubigenum and Lavandula angustifolia against the myiasis-inducing blowfly Lucilia sericata

et al. 2019

View full text Add to dashboard Cite

Cutaneous myiasis is a severe worldwide medical and veterinary issue. In this trial the essential oil (EO) of the Andean medicinal plant species Clinopodium nubigenum (Kunth) Kuntze was evaluated for its bioactivity against the myiasis-inducing blowfly Lucilia sericata (Meigen) (Diptera Calliphoridae) and compared with that of the well-known medicinal plant species Lavandula angustifolia Mill. The EOs were analysed and tested in laboratory for their oviposition deterrence and toxicity against L . sericata adults. The physiology of EO toxicity was evaluated by enzymatic inhibition tests. The antibacterial and antifungal properties of the EOs were tested as well. At 0.8 μL cm -2 , both EOs completely deterred L . sericata oviposition up to 3 hours. After 24 h, the oviposition deterrence was still 82.7% for L . angustifolia and the 89.5% for C . nubigenum . The two EOs were also toxic to eggs and adults of L . sericata . By contact/fumigation, the EOs, the LC 50 values against the eggs were 0.07 and 0.48 μL cm -2 while, by topical application on the adults, LD 50 values were 0.278 and 0.393 μL per individual for C . nubigenum and L . angustifolia EOs, respectively. Inhibition of acetylcholine esterase of L . sericata by EOs (IC 50 = 67.450 and 79.495 mg L -1 for C . nubigenum and L . angustifolia , respectively) suggested that the neural sites are targets of the EO toxicity. Finally, the observed antibacterial and antifungal properties of C . nubigenum and L . angustifolia EOs suggest that they could also help prevent secondary infections.

show abstract

Section: Introductionmentioning

confidence: 99%

Toxicity and oviposition deterrence of essential oils of Clinopodium nubigenum and Lavandula angustifolia against the myiasis-inducing blowfly Lucilia sericata

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The prevention of blowfly infestations has traditionally relied on synthetic insecticides such as organochlorines, organophosphates and insect growth regulators [ 17 , 18 ]. However, the repeated insurgence of blowfly resistance to chemicals [ 19 ] and, the issues around the harmful effects of synthetic compounds on humans [ 20 , 21 ], animals [ 22 ] and the environment [ 23 ], have made new eco-friendly, low-cost tools a high priority.…”

Section: Introductionmentioning

confidence: 99%

Artemisia spp. essential oils against the disease-carrying blowfly Calliphora vomitoria

et al. 2017

View full text Add to dashboard Cite

BackgroundSynanthropic flies play a considerable role in the transmission of pathogenic and non-pathogenic microorganisms. In this work, the essential oil (EO) of two aromatic plants, Artemisia annua and Artemisia dracunculus, were evaluated for their abilities to control the blowfly Calliphora vomitoria. Artemisia annua and A. dracunculus EOs were extracted, analysed and tested in laboratory bioassays. Besides, the physiology of EOs toxicity and the EOs antibacterial and antifungal properties were evaluated.ResultsBoth Artemisia EOs deterred C. vomitoria oviposition on fresh beef meat. At 0.05 μl cm-2 A. dracunculus EO completely inhibited C. vomitoria oviposition. Toxicity tests, by contact, showed LD50 of 0.49 and 0.79 μl EO per fly for A. dracunculus and A. annua, respectively. By fumigation, LC50 values were 49.55 and 88.09 μl l-1 air for A. dracunculus and A. annua, respectively. EOs AChE inhibition in C. vomitoria (IC50 = 202.6 and 472.4 mg l-1, respectively, for A. dracunculus and A. annua) indicated that insect neural sites are targeted by the EOs toxicity. Finally, the antibacterial and antifungal activities of the two Artemisia EOs may assist in the reduction of transmission of microbial infections/contaminations.ConclusionsResults suggest that Artemisia EOs could be of use in the control of C. vomitoria, a common vector of pathogenic microorganisms and agent of human and animal cutaneous myiasis. The prevention of pathogenic and parasitic infections is a priority for human and animal health. The Artemisia EOs could represent an eco-friendly, low-cost alternative to synthetic repellents and insecticides to fight synanthropic disease-carrying blowflies.

show abstract

“…Cyromazine was used widely in the sheep industry for over 30 years before Levot 7 reported that larvae recovered from a property in southern NSW showed resistance to this compound, and to dicyclanil to a lesser extent, in in vitro assays. Baker et al 8 subsequently showed that thorough application of cyromazine-and dicyclanil-based products at the label's recommended doses resulted in effective control of blowflies on this property, for periods consistent with the registered label claims. This indicated that the low level of in vitro resistance was not impacting the period of protection if the chemical was applied correctly.…”

Section: Insecticidesmentioning

confidence: 88%

Control of sheep flystrike: what's been tried in the past and where to from here

Kotze

James

2021

Aust Veterinary J

View full text Add to dashboard Cite

Flystrike remains a serious financial and animal welfare issue for the sheep industry in Australia despite many years of research into control methods. The present paper provides an extensive review of past research on flystrike, and highlights areas that hold promise for providing long-term control options. We describe areas where the application of modern scientific advances may provide increased impetus to some novel, as well as some previously explored, control methods. We provide recommendations for research activities: insecticide resistance management, novel delivery methods for therapeutics, improved breeding indices for flystrike-related traits, mechanism of nematode-induced scouring in mature animals. We also identify areas where advances can be made in flystrike control through the greater adoption of well-recognised existing management approaches: optimal insecticide-use patterns, increased use of flystrike-related Australian Sheep Breeding Values, and management practices to prevent scouring in young sheep. We indicate that breeding efforts should be primarily focussed on the adoption and improvement of currently available breeding tools and towards the future integration of genomic selection methods. We describe factors that will impact on the ongoing availability of insecticides for flystrike control and on the feasibility of vaccination. We also describe areas where the blowfly genome may be useful in providing impetus to some flystrike control strategies, such as area-wide approaches that seek to directly suppress or eradicate sheep blowfly populations. However, we also highlight the fact that commercial and feasibility considerations will act to temper the potential for the genome to act as the basis for providing some control options.

show abstract

Effective control of a suspected cyromazine‐resistant strain of Lucilia cuprina using commercial spray‐on formulations of cyromazine or dicyclanil

Abstract: Based on the results of these studies, there was no evidence of reduced susceptibility to cyromazine or dicyclanil and the periods of protection of sheep against L. cuprina were unaffected and consistent with the registered label claims.

Cited by 7 publications

References 12 publications

Toxicity and oviposition deterrence of essential oils of Clinopodium nubigenum and Lavandula angustifolia against the myiasis-inducing blowfly Lucilia sericata

Toxicity and oviposition deterrence of essential oils of Clinopodium nubigenum and Lavandula angustifolia against the myiasis-inducing blowfly Lucilia sericata

Artemisia spp. essential oils against the disease-carrying blowfly Calliphora vomitoria

Control of sheep flystrike: what's been tried in the past and where to from here

Contact Info

Product

Resources

About