Effective carrier-free gene-silencing activity of sphingosine-modified siRNAs

Abstract

RNA interference (RNAi) is a natural cellular process that silences the expression of target genes in a sequence-specific way by mediating targeted mRNA degradation. One of the main challenges in...

Non-terminal conjugation of small interfering RNAs with spermine improves duplex binding and serum stability with position-specific incorporation

“…However, most of the previous studies reported the conjugation at terminal position while some including lipid conjugation at non-terminal positions, to obtain effective gene silencing in carrier-free mode. [29][30][31][32][33] Therefore, we envisioned spermine modication at the non-terminal position by conjugation with a linker at the 4 ′ -C position of the modied nucleoside. We hypothesized that such a conjugated system would not alter the overall physical structure of the duplex and would exhibit conserved gene silencing with improved stability.…”