Effective Biotransformation of Variety of Guaiacyl Lignin Monomers Into Vanillin by Bacillus pumilus

Zuo, Kangjia; Li, Huanan; Chen, Jianhui; Ran, Qiuping; Huang, Mengtian; Cui, Xinxin; He, Lili; Liu, Jiashu; Jiang, Zhengbing

doi:10.3389/fmicb.2022.901690

Cited by 8 publications

(4 citation statements)

References 45 publications

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“…Isoeugenol was a much better substrate than eugenol, as determined from the biotransformations of standards. The activity of isoeugenol was confirmed in four monooxygenases of this strain [112].…”

Section: Scheme 23 Transformation Of Isoeugenol To Vanillin (A) By Is...mentioning

confidence: 63%

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Bio-Based Valorization of Lignin-Derived Phenolic Compounds: A Review

et al. 2023

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Lignins are the most abundant biopolymers that consist of aromatic units. Lignins are obtained by fractionation of lignocellulose in the form of “technical lignins”. The depolymerization (conversion) of lignin and the treatment of depolymerized lignin are challenging processes due to the complexity and resistance of lignins. Progress toward mild work-up of lignins has been discussed in numerous reviews. The next step in the valorization of lignin is the conversion of lignin-based monomers, which are limited in number, into a wider range of bulk and fine chemicals. These reactions may need chemicals, catalysts, solvents, or energy from fossil resources. This is counterintuitive to green, sustainable chemistry. Therefore, in this review, we focus on biocatalyzed reactions of lignin monomers, e.g., vanillin, vanillic acid, syringaldehyde, guaiacols, (iso)eugenol, ferulic acid, p-coumaric acid, and alkylphenols. For each monomer, its production from lignin or lignocellulose is summarized, and, mainly, its biotransformations that provide useful chemicals are discussed. The technological maturity of these processes is characterized based on, e.g., scale, volumetric productivities, or isolated yields. The biocatalyzed reactions are compared with their chemically catalyzed counterparts if the latter are available.

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Section: Scheme 23 Transformation Of Isoeugenol To Vanillin (A) By Is...mentioning

confidence: 63%

“…Scheme 24. Upgrade of bio-oil from masson pine via transformation of isoeugenol and eugenol to vanillin by whole cells of Bacillus pumilus [112]. The bio-oil also contained vanillin (15.9 mg/L) prior to biotransformation.…”

Section: Scheme 23 Transformation Of Isoeugenol To Vanillin (A) By Is...mentioning

confidence: 99%

Bio-Based Valorization of Lignin-Derived Phenolic Compounds: A Review

et al. 2023

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“…Although lignin-to-monomers conversion yields mostly in the range of 20–50 wt % have been demonstrated, ,− the development of high-performance microbes that can simultaneously catabolize sugars and lignin-derived intermediates is still in its very early stages. Most prior biological lignin upgrading work has focused on converting lignin monomers into commodity chemicals and high-value products, including indigoidine, vanillin, muconic acid, polyhydroxyalkanoates, methane, and lipids, using different host organisms such as Pseudomonas putida and Rhodococcus opacus. To avoid additional costs and energy associated with potential upgrading processes, a focus on bioconversion pathways that directly convert lignin monomers to targeted biofuels or biochemicals is essential.…”

Section: Resultsmentioning

confidence: 99%

Economic and Environmental Trade-Offs of Simultaneous Sugar and Lignin Utilization for Biobased Fuels and Chemicals

Baral,

Banerjee,

Mukhopadhyay

et al. 2023

ACS Sustainable Chem. Eng.

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Efficient lignin conversion is vital to the production of affordable, low-carbon fuels and chemicals from lignocellulosic biomass. However, lignin conversion remains challenging, and the alternative (combustion) can emit harmful air pollutants. This study explores the economic and environmental trade-offs between lignin combustion and microbial utilization for producing bisabolene as a representative biobased fuel or chemical. Results for switchgrass and clean pine-based biorefineries show that using lignin to increase fuel yields rather than combusting it reduces the capital expenditures for the boiler and turbogenerator if the facilities process more than 1100 bone-dry metric tons (bdt) feedstock/day and 560 bdt/day, respectively. No comparable advantage was observed for lower-lignin sorghum feedstock. Deconstructing lignin to bioavailable intermediates and utilizing those small molecules alongside sugars to boost product yields is economically attractive if the overall lignin-to-product conversion yield exceeds 11–20% by mass. Although lignin-to-fuel/chemical conversion can increase life-cycle greenhouse gas (GHG) emissions, most of the lignin can be diverted to fuel/chemical production while maintaining a >60% life-cycle GHG footprint reduction relative to diesel fuel. The results underscore that lignin utilization can be economically advantageous relative to combustion for higher-lignin feedstocks, but efficient depolymerization and high yields during conversion are both crucial to achieving viability.

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“…Bacillus pumilus , an endospore-forming Gram-positive bacterium, residing in stratospheric air, soil, deep-sea sediments, and some extreme environments, is one of the best-known Bacillus species ( Logan et al, 2009 ; Stepanov et al, 2016 ; Zhou et al, 2022 ). B. pumilus plays a crucial role in industry for producing abundant extracellular enzymes and secondary metabolites, and shows high adaptability and stress resistance ( Bonifer et al, 2019 ; Zuo et al, 2022 ). This species is commonly utilized as probiotics in animal ( Sanders et al, 2003 ; Hong et al, 2005 ).…”

Section: Introductionmentioning

confidence: 99%

Comparative genomic analyses reveal genetic characteristics and pathogenic factors of Bacillus pumilus HM-7

Wang

Zhang²,

Zhang³

et al. 2022

Front. Microbiol.

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Bacillus pumilus plays an important role in industrial application and biocontrol activities, as well as causing humans and plants disease, leading to economic losses and biosafety concerns. However, until now, the pathogenesis and underlying mechanisms of B. pumilus strains remain unclear. In our previous study, one representative isolate of B. pumilus named HM-7 has been recovered and proved to be the causal agent of fruit rot on muskmelon (Cucumis melo). Herein, we present a complete and annotated genome sequence of HM-7 that contains 4,111 coding genes in a single 3,951,520 bp chromosome with 41.04% GC content. A total of 3,481 genes were functionally annotated with the GO, COG, and KEGG databases. Pan-core genome analysis of HM-7 and 20 representative B. pumilus strains, as well as six closely related Bacillus species, discovered 740 core genes and 15,205 genes in the pan-genome of 21 B. pumilus strains, in which 485 specific-genes were identified in HM-7 genome. The average nucleotide identity (ANI), and whole-genome-based phylogenetic analysis revealed that HM-7 was most closely related to the C4, GR8, MTCC-B6033, TUAT1 and SH-B11 strains, but evolutionarily distinct from other strains in B. pumilus. Collinearity analysis of the six similar B. pumilus strains showed high levels of synteny but also several divergent regions for each strains. In the HM-7 genome, we identified 484 genes in the carbohydrate-active enzymes (CAZyme) class, 650 genes encoding virulence factors, and 1,115 genes associated with pathogen-host interactions. Moreover, three HM-7-specific regions were determined, which contained 424 protein-coding genes. Further investigation of these genes showed that 19 pathogenesis-related genes were mainly associated with flagella formation and secretion of toxic products, which might be involved in the virulence of strain HM-7. Our results provided detailed genomic and taxonomic information for the HM-7 strain, and discovered its potential pathogenic mechanism, which lay a foundation for developing effective prevention and control strategies against this pathogen in the future.

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Effective Biotransformation of Variety of Guaiacyl Lignin Monomers Into Vanillin by Bacillus pumilus

Cited by 8 publications

References 45 publications

Bio-Based Valorization of Lignin-Derived Phenolic Compounds: A Review

Bio-Based Valorization of Lignin-Derived Phenolic Compounds: A Review

Economic and Environmental Trade-Offs of Simultaneous Sugar and Lignin Utilization for Biobased Fuels and Chemicals

Comparative genomic analyses reveal genetic characteristics and pathogenic factors of Bacillus pumilus HM-7

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