Effective biodegradation on chicken feather by the recombinant KerJY-23 Bacillus subtilis WB600: A synergistic process coupled by disulfide reductase and keratinase

Enzyme-Induced “Sea–Island” Structure Reinforced Bio-Based Adhesive: Toward High Bonding Strength, Toughness, and Bacterial Resistance

“…29 Keratinase (Ker) is named for its ability to degrade S−S bonds in high keratin content biomass (such as animal hair), and it has a good hydrolysis effect on 7S/11S soy globulins under simple operations and mild conditions. 30 Consequently, the exposed −SH groups via enzymolysis facilitate interactions to "bridge" with thiolated organic and inorganic components, contributing to the creation of "sea−island" structure.…”

“…Unfolding the S–S bonds can expose more functional groups outward, enhancing the flexibility and reactivity of protein molecules, leading to the construction of strong “sea–island” structure. , Traditional methods for cleaving S–S bonds, such as sodium bisulfite, dithiothreitol, mercaptoethanol, and heat treatment, have some disadvantages, including high cost, complex processes, poor effectiveness in unfolding 7S/11S globulins of SP, and potential harm to humans . Keratinase (Ker) is named for its ability to degrade S–S bonds in high keratin content biomass (such as animal hair), and it has a good hydrolysis effect on 7S/11S soy globulins under simple operations and mild conditions . Consequently, the exposed −SH groups via enzymolysis facilitate interactions to “bridge” with thiolated organic and inorganic components, contributing to the creation of “sea–island” structure.…”