Effective and simple recovery of 1,3-propanediol from a fermented medium by liquid–liquid extraction system with ethanol and K 3 PO 4

Salting-out as a Fast and Simple Method for First-Step Separation of Biobased 1,3-Propanediol

“…However, it is important to note that both compounds were partitioned into an alcohol-rich phase. Wischral et al 26 found a partition coefficient value of 33 and a 97% recovery yield of 1,3-PDO from Clostridium beijerinckii DSM 791 A1 in salting-out systems with 28 wt % ethanol and 34 wt % K 3 PO 4 . Li et al 13 obtained K and Y% values of 38 and 98%, respectively, using salting-out extraction with methanol (35 wt %) and K 2 HPO 4 (40 wt %) from fermentation broth using Klebsiella pneumoniae CGMCC 2028.…”

“…Different inorganic salts (potassium phosphate tribasic, K 3 PO 4 ; potassium carbonate, K 2 CO 3 ; potassium phosphate dibasic, K 2 HPO 4 ; sodium phosphate monobasic, NaH 2 PO 4 ; and ammonium sulfate, (NH 4 ) 2 SO 4 ) and alcohols (ethanol and 2-propanol) were selected for the screening in the separation of 1,3-PDO. The equilibrium curve data used in this work were previously reported by Wischral et al, 26 Reis et al, 29 Santos et al, 30 and Aydogȃn et al 40 Briefly, a 30 g L −1 1,3-PDO solution was prepared to replicate the conditions found in the fermentation medium. Each system was prepared by choosing a mixture point within the two-phase region of the equilibrium curve by mixing together 55 wt % 1,3-PDO solution, 22.5 wt % alcohol, and 22.5 wt % salt, allowing the difference between the compositions of the two phases to be kept for a better comparison of the systems studied.…”

A CO2-responsive method for separating hydrophilic organic molecules from aqueous solutions: solvent-assisted switchable water

“…Unlike traditional salting-out systems reported in the literature, [24][25][26][27][28]34 the system containing TMBDA and PDO solution is capable of phase separation without the aid of an organic solvent (left edge of Fig. 2).…”

“…The low concen-tration combined with the high boiling point and high hydrophilicity make the recovery of PDO from water a great challenge resulting in high process costs due to the large amount of energy required to remove the water through traditional methods, such as, for example, by distillation. Many separation methods have already been proposed in an attempt to make the process more economically attractive, such as chromatography, [8][9][10][11][12] electrodialysis, 13,14 reactive extraction, [15][16][17][18][19][20] solvent extraction (liquid-liquid extraction), 21,22 salting-out (repulsive extraction), 23 salting-out extraction (SOE), [24][25][26][27][28] membrane filtration, 29,30 and distillation. 31 However, in addition to the energy expenditure, each of these processes have serious disadvantages that make unappealing for large scale implementation.…”

A novel downstream process for highly pure 1,3‐propanediol from an efficient fed‐batch fermentation of raw glycerol by Clostridium pasteurianum

“…Another strategy extensively evaluated for the separation of 1,3-PDO is two-phase salting-out extraction, which uses inorganic salts as salting-out reagents and organic solvents as extractants [18][19][20][21][22]. The solubility of 1,3-PDO in fermentation broth decreases significantly with the aid of a salting-out reagent, forcing 1,3-PDO to diffuse into the extractant.…”