Effective and Easy Techniques of Collagen Deposition onto Polylactide Films: DC-Discharge Plasma Treatment vs. Chemical Entrapment

Abstract

Enhancement of cell adhesion and growth on surface of the biodegradable materials is one of the important tasks in development of materials for regenerative medicine. This work focuses on comparison of various methods of collagen coating deposition onto polylactide films, aiming to increase their biocompatibility with human mesenchymal stromal cells. The collagen deposition was realized using either preliminary plasma treatment of the polylactide films or pre-swelling in solvent mixture. These techniques were compared in terms of the effect on the surface’s chemical structure, morphology, hydrophilicity and ability to support adhesion and growth of human mesenchymal stromal cells.

Nonlinear plasma surface modification of polylactide to promote interaction with fibroblasts

“…When processed for 5 min in the DBD plasma, the oxygen component O1s splits into two groups, in all likelihood, this is due to the charging of areas that are charged differently. 20 A comparison of the IR and XPS spectroscopy data of the studied PLA films indicates that the increase in the intensity of the bands of oscillations of polar C O bonds observed in the IR spectra, as well as an increase in the oxygen content in the XPS spectra with a relatively short plasma exposure time, is apparently explained, on the one hand, by additional polarization of these bonds with the appearance of a charge on the ether group (Figure 7A), on the other hand, the appearance of new oxygen-containing functional groups due to oxidative modification of the film surface (Figure 7B) under the action of primary radicals (H, OH, etc.) arising in the air atmosphere under the action of plasma.…”

“…Table 2 shows that for all oxygen‐containing bonds there is a nonlinearity from the modification time in the DBD plasma. When processed for 5 min in the DBD plasma, the oxygen component O1s splits into two groups, in all likelihood, this is due to the charging of areas that are charged differently 20 . At 5 min of plasma treatment, the XPS spectrum has 4 binding energies bonds: 532.4 eV—63% (OC), 535.4 eV—35%, 536.8 eV—23%, 531.2 eV—10% (OCN).…”