Effect on Polysaccharide Krestin from Coriolus versicolor Extract on Phagocytic Activity and Capacity of Mus musculus Exposed by Pseudomonas aeruginosa

Wahyuningsih, Sri Puji Astuti; Savira, Nadyatul Ilma Indah; Darmanto, Win

doi:10.15294/biosaintifika.v8i3.6957

Cited by 3 publications

(3 citation statements)

References 4 publications

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“…Novak and Vetvicka (2008) research showed that βglucan increased both proliferation of macrophage and cytokine in bone marrow, thus more phagocytes were recruited to the infected organs. Wahyuningsih et al (2016) stated that polysaccharide krestin from C. versicolor increased both phagocytic activity and capacity on Mus musculus exposed to Pseudomonas aeruginosa.…”

Section: Effect Of Crude Polysaccharides Of C Versicolor On Phagocytmentioning

confidence: 99%

CRUDE POLYSACCHARIDES EFFECT OF Coriolus versicolor ON Mycobacterium fortuitum-INDUCED IMMUNE DYSFUNTION IN MICE

Wahyuningsih

Winarni

Darmanto

et al. 2019

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Coriolus versicolor contains crude polysaccharides which have β-glucan active ingredients. It can activate granulocyte, monocyte, and macrophage. Administration of crude polysaccharide of C. versicolor against bacterial infection is hypothesized to increase immune response. This study assessed the crude polysaccharides activity of C. versicolor in improving immune response after induction of non-tuberculosis mycobacteria, Mycobacterium fortuitum. Animal model was female mice strain BALB/c aged 8-10 weeks. A crude polysaccharide of C. versicolor was administered before and/or after the bacteria infection for 10 days at a dose of 50 mg/kg body weight. Mice exposure to M. fortuitum was performed twice at a dose of 0.5 Mc. Farland. After crude polysaccharides treatment, both serum and peritoneal fluid were isolated. All data collected were analyzed statistically using ANOVA and Duncan test. Oral administration of crude polysaccharide was found to increase phagocyte number (P<0.05, from crude polysaccharides administration before-after infection), improve phagocytic activity (P<0.05, from crude polysaccharides administration before infection), raise both IFN- and antibody level (P<0.05, from crude polysaccharides administration after and before-after infection), and it caused TNF-α levels to tend to a normal concentration (P>0.05, TNF-α levels after crude polysaccharides administration were relatively the same as controls). Administration of crude polysaccharides of C. versicolor could enhance non-specific immune response, specific immune response, and pro-inflammatory cytokine in mice infected by M. fortuitum. These results suggested that crude polysaccharides of C. versicolor may be applied as an effective immunostimulatory agent.

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Section: Effect Of Crude Polysaccharides Of C Versicolor On Phagocytmentioning

confidence: 99%

CRUDE POLYSACCHARIDES EFFECT OF Coriolus versicolor ON Mycobacterium fortuitum-INDUCED IMMUNE DYSFUNTION IN MICE

Wahyuningsih

Winarni

Darmanto

et al. 2019

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“…From all infection cases in Indonesia, 7.1% was identified as nosocomial cases (World Health Organization, 2011) In recent years, using immunomodulator to enhance the immune response has proven to be an effective way to increase resistance to disease . Study conducted by Wahyuningsih et al (2016) showed a significant increase of phagocytic activity in treatment group treated by polysaccharides from Coriolus versicolor. Polysaccharides from plants have been known as an important immunostimulatory agent with broad spectrum, low toxicity and few side effects (Cho et al, 2014).…”

Section: Introductionmentioning

confidence: 97%

Okra Polysaccharides Improves Spleen Weight and B-Lymphocytes Proliferation in Mice Infected by Staphylococcus aureus

et al. 2017

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Okra (Abelmoschus esculentus) is reported to have various biological functions such as antioxidant, anticancer and anti-inflammation. However, only few studies have been reported immunomodulatory activities of okra to prevent disease caused by bacteria infection. In this study, the immunomodulatory activities of polysaccharides from okra pods were investigated further through the spleen weight and B-lymphocytes proliferation in mice infected by Staphylococcus aureus. Okra polysaccharides were obtained by water extraction and ethanol precipitation. Okra polysaccharides with doses of 25, 50, 75, and 100 mg/kg BW were orally administrated to mice with or without Staphylococcus aureus infection. Spleen weight was evaluated in both treatment group and control group. B-lymphocytes proliferation was evaluated by MTT assay using LPS induction. Results showed that okra polysaccharide at the doses of 50, 75, and 100 mg/kg increased spleen weight (p < 0.05) significantly. While at the dose of 75 and 100 mg/kg, it increased the B-lymphocytes proliferation (p < 0.05) significantly. There is positive correlation between the spleen weight and B-lymphocytes proliferation by 73.3%. These result reveal that okra polysaccharide could improve the immune response and be utilized as a novel candidate of neutraceutical.

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“…Wahyuningsih et al . [12] reported that PS of C. coriolus could enhance the response of immunoglobulin against Pseudomonas aeruginosa . Another study was also reported that polysaccharide from C. versicolor induce B-cell activation and enhance cytokine production [13].…”

Section: Introductionmentioning

confidence: 99%

Immunomodulatory potential of polysaccharides from Coriolus versicolor against intracellular bacteria Neisseria gonorrhoeae

Pramudya

Wahyuningsih

2019

Vet World

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Background and Aim: For many years, people use natural products from the plant and fungal to improve immune response against microorganism. This study aimed to investigate the immunomodulatory properties of polysaccharides (PS) from Coriolus versicolor in mice infected by intracellular bacteria Neisseria gonorrhoeae. Materials and Methods: Thirty-six female BALB/C mice were divided into six groups: Normal control, negative control, positive control, P1 (PS before infection), P2 (PS after infection), and P3 (PS before and after infection). PS were administrated for 10 days. N. gonorrhoeae was infected twice with 2 weeks gap from the first to second exposure with a dose of 106 cells. 1 week after the end of treatment, level of oxidants, innate immune responses, and adaptive immune responses were measured. Results: This study showed that PS administration could restore the number of leukocytes as normal but could not enhance the number of phagocytes and its activity. PS administration also showed immunosuppression activity by lowering nitric oxide levels in P2 and P3 groups (p<0.05). This result showed that PS prevent over-expression of pro-inflammatory cytokines by decreasing phagocytic activity. Contrast with innate immune response result; PS administration could significantly increase interferon-gamma level in P1, P2, and P3 groups (p<0.05). Level of antibodies was significantly increased in the P3 group (p<0.05). PS administration also showed an increased level of tumor necrosis factor-a, but the difference was not significant (p>0.05). Conclusion: PS enhance adaptive immunity due to the capability of N. gonorrhoeae that able to survive and replicate in phagocytes. Thus, PS from C. versicolor could be potentially be used as a natural immunomodulator against intracellular bacteria.

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Effect on Polysaccharide Krestin from Coriolus versicolor Extract on Phagocytic Activity and Capacity of Mus musculus Exposed by Pseudomonas aeruginosa

Cited by 3 publications

References 4 publications

CRUDE POLYSACCHARIDES EFFECT OF Coriolus versicolor ON Mycobacterium fortuitum-INDUCED IMMUNE DYSFUNTION IN MICE

CRUDE POLYSACCHARIDES EFFECT OF Coriolus versicolor ON Mycobacterium fortuitum-INDUCED IMMUNE DYSFUNTION IN MICE

Okra Polysaccharides Improves Spleen Weight and B-Lymphocytes Proliferation in Mice Infected by Staphylococcus aureus

Immunomodulatory potential of polysaccharides from Coriolus versicolor against intracellular bacteria Neisseria gonorrhoeae

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