Effect on Microbial Growth of a New Skin Protectant Formulation

Stoffel, Joseph; Bernatchez, Stéphanie F.

doi:10.1089/wound.2016.0706

Cited by 4 publications

(3 citation statements)

References 21 publications

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“…One limitation of this in vitro method would be that it does not simulate the wound healing process; thus, it cannot be studied through this model. Additionally, the agar plate may not reflect the clinical situation since it is different from the skin [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

“…Several studies also determined that the majority of infected wounds are colonized with at least 10 5 CFU/g of bacteria [ 36 , 52 – 58 ]. It has always been a matter of concern to strictly compare in vitro results from evaluating studies on tissue adhesives since the bacterial strains used, the inoculated volume, and CFU/mL values were different [ 33 ]. Bioluminescence bacteria have been utilized in many in vivo studies and showed many potentials to be used in different fields of science [ 59 – 63 ].…”

Section: Discussionmentioning

confidence: 99%

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A Novel In Vitro Method to Assess the Microbial Barrier Function of Tissue Adhesives Using Bioluminescence Imaging Technique

Mirzaei

Hagemeister²,

Hüffel

et al. 2022

BioMed Research International

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Background. Tissue glues can minimize treatment invasiveness, mitigate the risk of infection, and reduce surgery time; ergo, they have been developed and used in surgical procedures as wound closure devices beside sutures, staples, and metallic grafts. Regardless of their structure or function, tissue glues should show an acceptable microbial barrier function before being used in humans. This study proposes a novel in vitro method using Escherichia coli Lux and bioluminescence imaging technique to assess the microbial barrier function of tissue glues. Different volumes and concentrations of E. coli Lux were applied to precured or cured polyurethane-based tissue glue placed on agar plates. Plates were cultured for 1 h, 24 h, 48 h, and 72 h with bioluminescence signal measurement subsequently. Herein, protocol established a volume of 5 μL of a 1 : 100 dilution of E. coli Lux containing around 2 × 10 7 CFU/mL as optimal for testing polyurethane-based tissue glue. Measurement of OD600nm, determination of CFU/mL, and correlation with the bioluminescence measurement in p/s unit resulted in a good correlation between CFU/mL and p/s and demonstrated good reproducibility of our method. In addition, this in vitro method could show that the tested polyurethane-based tissue glue can provide a reasonable barrier against the microbial penetration and act as a bacterial barrier for up to 48 h with no penetration and up to 72 h with a low level of penetration through the material. Overall, we have established a novel, sensitive, and reproducible in vitro method using the bioluminescence imaging technique for testing the microbial barrier function of new tissue glues.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A Novel In Vitro Method to Assess the Microbial Barrier Function of Tissue Adhesives Using Bioluminescence Imaging Technique

Mirzaei

Hagemeister²,

Hüffel

et al. 2022

BioMed Research International

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“…Cyanoacrylates have been shown to provide a barrier to bacterial penetration. 25 Therefore, we used absorbable suture to suture most of the subcutaneous tissue and closed the wound with cyanoacrylate glue.…”

Section: Discussionmentioning

confidence: 99%

Risk factors of infection of totally implantable venous access port: A retrospective study

et al. 2022

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Background: Infection is the most frequent complication associated with the use of totally implantable venous access port (TIVAP). This retrospective study was conducted to determine the risk factors affecting TIVAP-related infection. Methods: A total of 1406 patients implanted with TIVAP at our center were included in this retrospective study. Incidence of perioperative infection, patient characteristics and bacteriologic data were retrieved and analyzed. Univariable analyses and multiple logistic regression analyses were used to determine the risk factors. Results: Overall, 72 (5.1%) patients had perioperative infection, and TIVAP was finally removed from 12 (0.85%) patients. There was significantly more hematologic malignancy in the infection group, compared to the non-infection group. Patients with chemotherapy and infection within 30 days before operation also had more infections. There were more inpatients in the infection group than in the non-infection group. The rate of hematoma was higher in the infected patients. Multivariate logistic analysis revealed that hematoma (OR 5.695, p < 0.001), preoperative hospital stay (⩾14d) (OR 2.945, p < 0.001), history of chemotherapy (OR 2.628, p = 0.002), history of infection (within 30 days) (OR 4.325, p < 0.001) were independent risk factor for infection. Conclusions: This study demonstrated that hematoma, preoperative hospital stay (⩾14d), history of chemotherapy and history of infection (within 30 days) are independent risk factor for all patients.

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Nanostructured Cyanoacrylates: Biomedical Applications

Bayer

2018

Smart Nanoparticles for Biomedicine

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Effect on Microbial Growth of a New Skin Protectant Formulation

Cited by 4 publications

References 21 publications

A Novel In Vitro Method to Assess the Microbial Barrier Function of Tissue Adhesives Using Bioluminescence Imaging Technique

A Novel In Vitro Method to Assess the Microbial Barrier Function of Tissue Adhesives Using Bioluminescence Imaging Technique

Risk factors of infection of totally implantable venous access port: A retrospective study

Nanostructured Cyanoacrylates: Biomedical Applications

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