Effect of γ-Irradiation on the Antibacterial Activities ofCuminum cyminum L.Essential OilsIn VitroandIn VivoSystems

Abstract

The present study was carried out to investigate the effect of γ-irradiation at two irradiation doses of 10 and 25 kGy, on the antibacterial activities of cumin essential oils (E.Os) in vitro and in vivo systems. For this purpose, the cumin seeds were irradiated with Co 60 source (0, 10 and 25 kGy) and subjected to Clevenger extraction to obtain E.Os. In vitro antibacterial activities of cumin E.Os was determined on Gram-negative and Gram-positive bacterial strains including E. coli, P. aeroginosa, B. cereus and S. aureus using agar well diffusion method, disk diffusion method and, MIC and MBC determinations. In vivo antibacterial activity was performed on polymicrobial infection induced by cecal ligation and puncture (CLP) in rat through blood colony forming units (CFUs) determination. The results showed that CLP operation caused a marked increase in bacterial load of blood which was reduced in rats treated with fresh and irradiated cumin oils. In vitro results also showed the sensitivity of all tested bacterial strains against the fresh and irradiated cumin E.Os. These data may suggest that cumin E.O has potential antibacterial activity both in vivo and in vitro systems. Also, γ-irradiation to cumin seeds at 10 and 25 kGy has no significant effects on the antibacterial properties of cumin E.Os.

Antibacterial and Antifungal Activities of Spices

“…Cumin has been used since ancient time as an ingredient in foods in Middle East, and cumin seeds have long been used as antiseptic and disinfectant in India [80]. Cuminaldehyde, cymene, and terpenoids are the major bioactive constituents of cumin EOs [81].…”

“…Both microwave and conventionally (oven) roasted cumin oils had similar antimicrobial effects on microorganisms tested and were more effective than those of raw oils. Reza et al [80] studied the effects of ã-irradiation (10 and 25 kGy) on the antibacterial activities of cumin against E . coli , P .…”

Essential Oils and Their Natural Active Compounds Presenting Antifungal Properties

“…Well known for its use as a spice, cumin ( Cuminum cyminum ) has also been long used as an antiseptic and disinfectant, particularly in India. [53] At present, it is also being studied for its antifungal activity. The study by Kedia et al on 1230 fungal isolates from food samples, including various strains of Aspergillus spp and Fusarium spp in particular, demonstrated an activity against these strains with a concentration of 0.6 μL/mL of cumin essential oil [54].…”

Antibacterial and antiseptic activities of various spices to the mala food ingredients

“…The three main compounds of cinnamon are cinnamaldehyde, cinnamyl acetate and cinnamyl alcohol (Khasnavis & Pahan, 2012) and due to their antibacterial activities, cinnamon is used in cosmetics or food products (Nabavi et al, 2015) as well as to treat diseases such as inflammation, gastrointestinal disorders and urinary infections (Brierley & Kelber, 2011;Al-Jiffri et al, 2011). Cumin (Cuminum cyminum) has been used as food ingredients in the Middle East since ancient time and cumin seeds have been used as antiseptic and disinfectant in India (Mohammad Reza et al, 2015;Feng et al, 2020). The major bioactive components of cumin essential oils are cuminaldehyde, cymene and terpenoids (Allahghadri et al, 2010).…”